F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins." alt="HEK293 human embryonic kidney cell line transfected with either (A) human B7-H7/HHLA2 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human B7-H7/HHLA2 Monoclonal Antibody (Catalog # MAB80841) followed by anti-Mouse IgG APC-conjugated secondary antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB80842-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-49187-25ul | |||
HAF017). Specific bands were detected for B7-H7/HHLA2 at approximately 90-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HEK293T human embryonic kidny cell line transfected with human B7-H7. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human B7-H7/HHLA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8084) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for B7-H7/HHLA2 at approximately 90-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF8084-SP | |||
8316-TR) binds to HEK293 human embryonic kidney cell line transfected with human B7-H7/HHLA2 (black dotted line). Binding is completely blocked (orange histogram) by 2.5 µg/mL of Mouse Anti-Human B7-H7/HHLA2 Monoclonal Antibody (Catalog # MAB80841R). Mouse IgG1 Isotype Control (Catalog # MAB002) at 2.5 µg/mL was used as a control (blue line). Cells were stained with Streptavidin-APC (Catalog # F0050)." alt="In a functional flow cytometry test, biotinylated recombinant human TMIGD2/CD28H (10 ng/mL, Catalog # 8316-TR) binds to HEK293 human embryonic kidney cell line transfected with human B7-H7/HHLA2 (black dotted line). Binding is completely blocked (orange histogram) by 2.5 µg/mL of Mouse Anti-Human B7-H7/HHLA2 Monoclonal Antibody (Catalog # MAB80841R). Mouse IgG1 Isotype Control (Catalog # MAB002) at 2.5 µg/mL was used as a control (blue line). Cells were stained with Streptavidin-APC (Catalog # F0050)." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB80841R-SP | |||
| Carrier Free | FAB80841RV-100UG | |||
| Carrier Free | FAB80841RU-100UG | |||
| Carrier Free | FAB80841RT-100UG | |||
| Carrier Free | FAB80841RS-100UG | |||
| Carrier Free | FAB80841RR-100UG | |||
| Carrier Free | FAB80841RN-100UG | |||
| Carrier Free | FAB80841RG-100UG | |||
NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="B7-H7/HHLA2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-HumanB7-H7/HHLA2 Monoclonal Antibody (Catalog # MAB8084) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB8084-SP | |||
NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for 
8316-TR) binds to HEK293 human embryonic kidney cell line transfected with human B7-H7/ HHLA2 (black dotted line). Binding is completely blocked (orange histogram) by 2.5 µg/mL of Mouse Anti-Human B7-H7/ HHLA2 Monoclonal Antibody (Catalog # MAB80841). Mouse IgG1 Isotype Control (Catalog # 
8316-TR), coated at 10 ng/mL binds to Recombinant Human B7-H7/HHLA2 Fc Chimera (Catalog # ![Flow Cytometry: B7-H7/HHLA2 Antibody (907834) - Azide Free [MAB80842] - An intracellular stain was performed on HeLa cells with B7-H7/HHLA2 [907834] Antibody MAB80842AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/B7-H7-HHLA2-Antibody-907834-Azide-Free-Flow-Cytometry-MAB80842-img0002.jpg "Flow Cytometry: B7-H7/HHLA2 Antibody (907834) - Azide Free [MAB80842] - An intracellular stain was performed on HeLa cells with B7-H7/HHLA2 [907834] Antibody MAB80842AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.")
![Flow Cytometry: B7-H7/HHLA2 Antibody (907834) - Azide Free [MAB80842] - An intracellular stain was performed on Caco-2 cells with B7-H7/HHLA2 Antibody [907834] MAB80842AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/B7-H7-HHLA2-Antibody-907834-Azide-Free-Flow-Cytometry-MAB80842-img0001.jpg)
![Immunohistochemistry-Paraffin: B7-H7/HHLA2 Antibody [NBP2-49187] - Analysis in human colon and liver tissues using NBP2-49187 antibody. Corresponding B7-H7/HHLA2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/B7-H7-HHLA2-Antibody-Immunohistochemistry-Paraffin-NBP2-49187-img0016.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: B7-H7/HHLA2 Antibody [NBP2-49187] - Analysis in human colon and liver tissues using NBP2-49187 antibody. Corresponding B7-H7/HHLA2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: B7-H7/HHLA2 Antibody [NBP2-49187] - Analysis in human cell line HDLM-2.](http://images.novusbio.com/fullsize/B7-H7-HHLA2-Antibody-Western-Blot-NBP2-49187-img0015.jpg)
![Immunohistochemistry-Paraffin: B7-H7/HHLA2 Antibody [NBP2-49187] - Staining of human colon shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/B7-H7-HHLA2-Antibody-Immunohistochemistry-Paraffin-NBP2-49187-img0011.jpg)
![Immunohistochemistry-Paraffin: B7-H7/HHLA2 Antibody [NBP2-49187] - Staining of human kidney shows weak cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/B7-H7-HHLA2-Antibody-Immunohistochemistry-Paraffin-NBP2-49187-img0012.jpg)
![Immunohistochemistry-Paraffin: B7-H7/HHLA2 Antibody [NBP2-49187] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/B7-H7-HHLA2-Antibody-Immunohistochemistry-Paraffin-NBP2-49187-img0013.jpg)
![Immunohistochemistry-Paraffin: B7-H7/HHLA2 Antibody [NBP2-49187] - Staining of human small intestine shows moderate to strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/B7-H7-HHLA2-Antibody-Immunohistochemistry-Paraffin-NBP2-49187-img0014.jpg)
NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for 
F0101B). Quadrant markers were set based on control antibody staining (Catalog # 
