B7-2/CD86 Antibodies

VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="B7‑2/CD86 was detected in immersion fixed paraffin-embedded sections of mouse spleen using Rat Anti-Mouse B7‑2/CD86 Monoclonal Antibody (Catalog # mab11469) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes in appendix. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes in appendix. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="B7‑2/CD86 was detected in immersion fixed paraffin-embedded sections of human appendix using Mouse Anti-Human B7‑2/CD86 Monoclonal Antibody (Catalog # MAB108521) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes in appendix. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

1340-B2) co-stimulates IL-2 secretion in the Jurkat human acute T cell leukemia cell line in the presence of PHA in a dose-dependent manner (orange line), as measured by the Human IL-2 Quantikine ELISA Kit (Catalog # D2050). IL-2 secretion elicited by Recombinant Rat B7-2/CD86 Fc Chimera (1 µg/mL) and PHA (10 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat B7-2/CD86 Monoclonal Antibody (Catalog # MAB13401). The ND₅₀ is typically 0.3-1.0 µg/mL." class="big_lightbox"> 1340-B2) co-stimulates IL-2 secretion in the Jurkat human acute T cell leukemia cell line in the presence of PHA in a dose-dependent manner (orange line), as measured by the Human IL-2 Quantikine ELISA Kit (Catalog # D2050). IL-2 secretion elicited by Recombinant Rat B7-2/CD86 Fc Chimera (1 µg/mL) and PHA (10 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat B7-2/CD86 Monoclonal Antibody (Catalog # MAB13401). The ND₅₀ is typically 0.3-1.0 µg/mL." alt="Recombinant Rat B7-2/CD86 Fc Chimera (Catalog # 1340-B2) co-stimulates IL-2 secretion in the Jurkat human acute T cell leukemia cell line in the presence of PHA in a dose-dependent manner (orange line), as measured by the Human IL-2 Quantikine ELISA Kit (Catalog # D2050). IL-2 secretion elicited by Recombinant Rat B7-2/CD86 Fc Chimera (1 µg/mL) and PHA (10 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat B7-2/CD86 Monoclonal Antibody (Catalog # MAB13401). The ND₅₀ is typically 0.3-1.0 µg/mL." class="big_thumb" />

9090-B2) coated at 0.5 μg/mL (100 µL/well). At 1.0 μg/mL, this antibody will block >90% of the binding." class="big_lightbox"> 9090-B2) coated at 0.5 μg/mL (100 µL/well). At 1.0 μg/mL, this antibody will block >90% of the binding." alt="In a functional ELISA binding assay, 15.0-150 ng/mL of this antibody will block 50% of the binding of 200 ng/mL of recombinant human CTLA-4/Fc (7868-CT) to immobilized recombinant human B7-2/His (9090-B2) coated at 0.5 μg/mL (100 µL/well). At 1.0 μg/mL, this antibody will block >90% of the binding." class="big_thumb" />