VSIG3/IGSF11 Antibodies

HAF008). A specific band was detected for VSIG3 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF008). A specific band was detected for VSIG3 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

F0101B). Quadrant markers were set based on isotype control antibody staining (MAB0041, data not shown). Staining was performed using our Staining Membrane-Associated Proteins protocol." class="big_lightbox"> F0101B). Quadrant markers were set based on isotype control antibody staining (MAB0041, data not shown). Staining was performed using our Staining Membrane-Associated Proteins protocol." alt="HEK293 human embryonic kidney cell line transfected with either (A) human VSIG3 or (B) irrelevant protein, and eGFP, was stained with Mouse anti-Human VSIG3 monoclonal antibody (Catalog # MAB92293) followed by Allophycocyanin-conjugated anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on isotype control antibody staining (MAB0041, data not shown). Staining was performed using our Staining Membrane-Associated Proteins protocol." class="big_thumb" />

9229-VS) to immobilized Recombinant Human VISTA/B7-H5/PD-1H Fc Chimera (7126-B7) coated at 1 µg/mL (100 µL/well). At 10 µg/mL, this antibody will block >90% of the binding." class="big_lightbox"> 9229-VS) to immobilized Recombinant Human VISTA/B7-H5/PD-1H Fc Chimera (7126-B7) coated at 1 µg/mL (100 µL/well). At 10 µg/mL, this antibody will block >90% of the binding." class="big_thumb" />

F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins." alt="HEK293 human embryonic kidney cell line transfected with either (A) human VSIG3 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human VSIG3 Monoclonal Antibody (Catalog # MAB9229) followed by APC-conjugated Goat-anti Mouse IgG secondary antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

DY990). Sheep Anti-Human/Mouse VSIG3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4915) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox"> DY990). Sheep Anti-Human/Mouse VSIG3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4915) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />

MAB9229) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human/Mouse VSIG3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4915) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox"> MAB9229) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human/Mouse VSIG3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4915) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Human VSIG3 protein was serially diluted 2-fold and captured by Mouse Anti-Human VSIG3 Monoclonal Antibody (Catalog # MAB9229) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human/Mouse VSIG3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4915) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />

F0101B). Quadrant markers were set based on Mouse IgG1 Isotype Control (Catalog # MAB002, data not shown). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0101B). Quadrant markers were set based on Mouse IgG1 Isotype Control (Catalog # MAB002, data not shown). View our protocol for Staining Membrane-associated Proteins." alt="HEK293 human embryonic kidney cell line transfected with (A) Human VSIG3 or (B) irrelevant protein, and EGFP, was stained with Mouse Anti-Human VSIG3 Monoclonal Antibody (Catalog # MAB92292) followed by anti-Mouse IgG APC-conjugated secondary antibody (Catalog # F0101B). Quadrant markers were set based on Mouse IgG1 Isotype Control (Catalog # MAB002, data not shown). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in glandular cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in glandular cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="VSIG3 was detected in immersion fixed paraffin-embedded sections of human colon using Rabbit Anti-Human VSIG3 Monoclonal Antibody (Catalog # MAB92294) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in glandular cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

9229-VS) to immobilized Recombinant Human VISTA/B7-H5/PD-1H Fc Chimera (Catalog # 7126-B7) coated at 1 µg/mL (100 µL/well). At 10 µg/mL, this antibody will block >90% of the binding." class="big_lightbox"> 9229-VS) to immobilized Recombinant Human VISTA/B7-H5/PD-1H Fc Chimera (Catalog # 7126-B7) coated at 1 µg/mL (100 µL/well). At 10 µg/mL, this antibody will block >90% of the binding." alt="In a functional ELISA, 0.2-2 µg/mL of this antibody will block 50% of the binding of 1 µg/mL of Recombinant Human VSIG3 Fc Chimera (Catalog # 9229-VS) to immobilized Recombinant Human VISTA/B7-H5/PD-1H Fc Chimera (Catalog # 7126-B7) coated at 1 µg/mL (100 µL/well). At 10 µg/mL, this antibody will block >90% of the binding." class="big_thumb" />