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![Dot Blot: 3-methylcytosine (3-mC) Antibody (PSH03-21) [NBP3-31984] - Dot blot analysis of 3-methylcytosine (3-mC) on different proteins with Rabbit anti-3mC antibody (NBP3-31984) at 1/2,000 dilution. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution for 1 hour at room temperature. Lane 1: Uridine-BSA (negative) Lane 2: Cytidine-BSA (negative) Lane 3: 5-Methylcytosine-BSA (negative) Lane 4: 3-Methylcytosine-BSA (positive) Lane 5: 5-Hydroxymethylcytidine-BSA (negative) Proteins loading: 50ng, 25ng, 12.5ng, 6.25ng; Blocking and dilution buffer: 5% NFDM/TBST; Exposure time: 1 minute.](http://images.novusbio.com/fullsize/nbp3-31984_rabbit-3-methylcytosine-3-mc-mab-psh03-21-28820241459252.jpg "Dot Blot: 3-methylcytosine (3-mC) Antibody (PSH03-21) [NBP3-31984] - Dot blot analysis of 3-methylcytosine (3-mC) on different proteins with Rabbit anti-3mC antibody (NBP3-31984) at 1/2,000 dilution. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution for 1 hour at room temperature. Lane 1: Uridine-BSA (negative) Lane 2: Cytidine-BSA (negative) Lane 3: 5-Methylcytosine-BSA (negative) Lane 4: 3-Methylcytosine-BSA (positive) Lane 5: 5-Hydroxymethylcytidine-BSA (negative) Proteins loading: 50ng, 25ng, 12.5ng, 6.25ng; Blocking and dilution buffer: 5% NFDM/TBST; Exposure time: 1 minute.")
![Immunocytochemistry/ Immunofluorescence: 3-methylcytosine (3-mC) Antibody (PSH03-21) [NBP3-31984] - Immunocytochemistry analysis of U-2 OS cells treated with 4mM Methyl Methanesulfonate for 1 hour labeling 3-methylcytosine (3-mC) with Rabbit anti-3-methylcytosine (3-mC) antibody (NBP3-31984) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-3-methylcytosine (3-mC) antibody (NBP3-31984) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-31984_rabbit-3-methylcytosine-3-mc-mab-psh03-21-29202410334042.jpg)
![Dot Blot: 3-methylcytosine (3-mC) Antibody (PSH03-22) [NBP3-31985] - Dot blot analysis of 3-methylcytosine (3-mC) on different proteins with Rabbit anti-3mC antibody (NBP3-31985) at 1/2,000 dilution. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution for 1 hour at room temperature. Lane 1: Uridine-BSA (negative) Lane 2: Cytidine-BSA (negative) Lane 3: 5-Methylcytosine-BSA (negative) Lane 4: 3-Methylcytosine-BSA (positive) Lane 5: 5-Hydroxymethylcytidine-BSA (negative) Proteins loading: 50ng, 25ng, 12.5ng, 6.25ng; Blocking and dilution buffer: 5% NFDM/TBST; Exposure time: 1 minute.](http://images.novusbio.com/fullsize/nbp3-31985_rabbit-3-methylcytosine-3-mc-mab-psh03-22-28820241459253.jpg "Dot Blot: 3-methylcytosine (3-mC) Antibody (PSH03-22) [NBP3-31985] - Dot blot analysis of 3-methylcytosine (3-mC) on different proteins with Rabbit anti-3mC antibody (NBP3-31985) at 1/2,000 dilution. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution for 1 hour at room temperature. Lane 1: Uridine-BSA (negative) Lane 2: Cytidine-BSA (negative) Lane 3: 5-Methylcytosine-BSA (negative) Lane 4: 3-Methylcytosine-BSA (positive) Lane 5: 5-Hydroxymethylcytidine-BSA (negative) Proteins loading: 50ng, 25ng, 12.5ng, 6.25ng; Blocking and dilution buffer: 5% NFDM/TBST; Exposure time: 1 minute.")
![Immunocytochemistry/ Immunofluorescence: 3-methylcytosine (3-mC) Antibody (PSH03-22) [NBP3-31985] - Immunocytochemistry analysis of U-2 OS cells treated with 4mM Methyl Methanesulfonate for 1 hour labeling 3-methylcytosine (3-mC) with Rabbit anti-3-methylcytosine (3-mC) antibody (NBP3-31985) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-3-methylcytosine (3-mC) antibody (NBP3-31985) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-31985_rabbit-3-methylcytosine-3-mc-mab-psh03-22-29202410334091.jpg)
![Dot Blot: 3-methylcytosine (3-mC) Antibody [NBP2-62790] - Analysis was performed using synthetic oligonucleotides containing different modified bases to demonstrate the specificity of the antibody against 3-mC. This figure shows a high specificity of the antibody for the oligonucleotide with the 3-methylcytosine modification.](http://images.novusbio.com/fullsize/3-methylcytosine-3-mC-Antibody-Dot-Blot-NBP2-62790-img0001.jpg "Dot Blot: 3-methylcytosine (3-mC) Antibody [NBP2-62790] - Analysis was performed using synthetic oligonucleotides containing different modified bases to demonstrate the specificity of the antibody against 3-mC. This figure shows a high specificity of the antibody for the oligonucleotide with the 3-methylcytosine modification.")