| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82549-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3626-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-47375-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | 3626-ML-010/CF | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-164SS | |||
| Azide and BSA Free | NBP2-80593 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF591-SP | |||
| Carrier Free | FAB591G-100UG | |||
| Carrier Free | FAB591R-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71650 | |||
![Western Blot: RP2 Antibody [NBP1-56852] - Titration: 0.2-1 ug/ml, Positive Control: Human Muscle.](http://images.novusbio.com/fullsize/RP2-Antibody-Western-Blot-NBP1-56852-img0001.jpg "Western Blot: RP2 Antibody [NBP1-56852] - Titration: 0.2-1 ug/ml, Positive Control: Human Muscle.")
![Immunohistochemistry-Paraffin: RP2 Antibody [NBP1-56852] - Human Brain, cerebellum tissue at an antibody concentration of 5ug/ml.](http://images.novusbio.com/fullsize/RP2-Antibody-Immunohistochemistry-Paraffin-NBP1-56852-img0004.jpg)
![Western Blot: RP2 Antibody [NBP1-82549] - Analysis in human cell line BEWO.](http://images.novusbio.com/fullsize/RP2-Antibody-Western-Blot-NBP1-82549-img0004.jpg "Western Blot: RP2 Antibody [NBP1-82549] - Analysis in human cell line BEWO.")
![Immunohistochemistry-Paraffin: RP2 Antibody [NBP1-82549] - Staining of human appendix shows moderate cytoplasmic positivity in lymphoid tissue and glandular cells.](http://images.novusbio.com/fullsize/RP2-Antibody-Immunohistochemistry-Paraffin-NBP1-82549-img0003.jpg)
![Immunohistochemistry-Paraffin: MiRP2 Antibody [NBP1-49885] - 3.75ug/ml staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/MiRP2-Antibody-Immunohistochemistry-Paraffin-NBP1-49885-img0001.jpg "Immunohistochemistry-Paraffin: MiRP2 Antibody [NBP1-49885] - 3.75ug/ml staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Immunocytochemistry/Immunofluorescence: MGAM2 Antibody [NBP2-47375] - Staining of human cell line RH-30 shows localization to cytosol.](http://images.novusbio.com/fullsize/MGAM2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47375-img0002.jpg "Immunocytochemistry/Immunofluorescence: MGAM2 Antibody [NBP2-47375] - Staining of human cell line RH-30 shows localization to cytosol.")
![Immunohistochemistry-Paraffin: MGAM2 Antibody [NBP2-47375] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/MGAM2-Antibody-Immunohistochemistry-Paraffin-NBP2-47375-img0004.jpg)
![Immunohistochemistry-Paraffin: MGAM2 Antibody [NBP2-47375] - Staining of human duodenum shows high expression.](http://images.novusbio.com/fullsize/MGAM2-Antibody-Immunohistochemistry-Paraffin-NBP2-47375-img0003.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: MGAM2 Antibody [NBP2-47375] - Staining in human duodenum and liver tissues using anti-MGAM2 antibody. Corresponding MGAM2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MGAM2-Antibody-Immunohistochemistry-Paraffin-NBP2-47375-img0005.jpg)
![Western Blot: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Detection of Human Phospho RPA32 (S4/S8) by western blot. Samples: Whole cell lysate (50 ug) from HeLa cells treated with 100 uM EPE (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) antibody NBP1-23017 used at 0.1 ug/ml. Lower panel: Rabbit anti-RPA32 antibody NB100-332. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/RPA2-[p-Ser4--p-Ser8]-Antibody-Western-Blot-NBP1-23017-img0047.jpg "Western Blot: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Detection of Human Phospho RPA32 (S4/S8) by western blot. Samples: Whole cell lysate (50 ug) from HeLa cells treated with 100 uM EPE (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) antibody NBP1-23017 used at 0.1 ug/ml. Lower panel: Rabbit anti-RPA32 antibody NB100-332. Detection: Chemiluminescence with an exposure time of 75 seconds.")
![Immunocytochemistry/Immunofluorescence: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Formaldehyde-fixed asynchronous HeLa cells. Untreated cells (left) and EPE treated cells (right). Antibody: Affinity purified rabbit anti- Phospho RPA32 (S4/S8) used at a dilution of 1:1,000 (1ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100. Counterstain: Alexa Fluor (R) 488 phalloidin (green).](http://images.novusbio.com/fullsize/RPA2-[p-Ser4--p-Ser8]-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-23017-img0046.jpg)
![Immunohistochemistry-Paraffin: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Section of mouse gut, MOCK treatment (left) and CIP treatment (right).Antibody: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/RPA2-[p-Ser4--p-Ser8]-Antibody-Immunohistochemistry-Paraffin-NBP1-23017-img0050.jpg)
![Western Blot: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Samples: Whole cell lysate (50 ug for WB in A; 1 mg for IP, 20% of IP loaded in B) from HeLa Cells that had been treated with EPE (+) or mock treated (-). In B, immunoprecipitated RPA32 was either treated with phosphatase (+) or mock treated (-). Antibodies: Phosphorylated RPA32 was detected using rabbit anti-phospho RPA32 Ser4/Ser8 antibody NBP1-23017 lot 2 at 1 ug/ml. Total RPA32 was detected using rabbit anti-RPA32 antibody NB100-332 at 1 ug/ml. RPA32 was immunoprecipitated using NB100-332 at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds for phospho RPA32 Ser4/Ser8 (A and B) and 1 second for total RPA32 (A and B).](http://images.novusbio.com/fullsize/RPA2-[p-Ser4--p-Ser8]-Antibody-Western-Blot-NBP1-23017-img0003.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Detection of Human Phospho RPA32 (S4/S8) by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa cells treated with 100 uM EPE for 4 hours (+) or mock treated (-) cells. Antibodies: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) antibody NBP1-23017 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower panel shows Westen Blot for total RPA32 using affinity purified rabbit anti-RPA32 antibody A300-244A at 0.1 ug/ml with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/RPA2-[p-Ser4--p-Ser8]-Antibody-Western-Blot-NBP1-23017-img0042.jpg)
![Immunohistochemistry-Paraffin: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Section of human breast carcinoma, MOCK treatment (left) and CIP treatment(right). Antibody: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) (used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/RPA2-[p-Ser4--p-Ser8]-Antibody-Immunohistochemistry-Paraffin-NBP1-23017-img0049.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Detection of human Phospho RPA32 (S4/S8) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells treated with EPE (+) and/or Lambda PP (+) or mock treated (-). Antibodies: RPA32 (S4/S8) was immunoprecipitated using affinity purified rabbit anti-RPA32 antibody NB100-332. For blotting immunoprecipitated Phospho RPA32 (S4/S8), rabbit anti-Phospho RPA32 (S4/S8) antibody NBP1-23017 was used at 0.1 ug/ml. Lower Panel: Rabbit anti-RPA antibody NB100-332. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/RPA2-[p-Ser4--p-Ser8]-Antibody-Immunoprecipitation-NBP1-23017-img0048.jpg)
![Western Blot: Bestrophin 1 Antibody (E6-6) - BSA Free [NB300-164] - Western blot analysis of the normal and mutant human Best1 protein in transiently transfected MDCK cells. Best1 proteins are detectable as a 68 kDa band in all transfected cells, but not in non-transfected controls (MDCK lane). Actin bands are shown to indicate equal loading of cell lysates. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/14/7/15121), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Bestrophin-1-Antibody-E6-6-BSA-Free-Western-Blot-NB300-164-img0007.jpg "Western Blot: Bestrophin 1 Antibody (E6-6) - BSA Free [NB300-164] - Western blot analysis of the normal and mutant human Best1 protein in transiently transfected MDCK cells. Best1 proteins are detectable as a 68 kDa band in all transfected cells, but not in non-transfected controls (MDCK lane). Actin bands are shown to indicate equal loading of cell lysates. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/14/7/15121), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Bestrophin 1 Antibody (E6-6) - BSA Free [NB300-164] - X-Z confocal single image scan of transiently transfected cells with different BEST1 cDNA constructs showing mislocalization of mutants Y85H, Q96R, L100R and Y227N. Cells were stained for Best1 (green), beta-catenin (red) and nuclei (blue). Scale bar = 10 um. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/14/7/15121), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Bestrophin-1-Antibody-E6-6-BSA-Free-Immunocytochemistry-Immunofluorescence-NB300-164-img0008.jpg)
![Immunohistochemistry-Paraffin: Bestrophin 1 Antibody (E6-6) - BSA Free [NB300-164] - Bestrophin 1 was detected in immersion fixed paraffin sections of human small intestine using t Mouse Anti-Human Bestrophin 1 Monoclonal Antibody (Catalog # NB300-164) at 5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface and extracellular.](http://images.novusbio.com/fullsize/Bestrophin-1-Antibody-E6-6-BSA-Free-Immunohistochemistry-Paraffin-NB300-164-img0005.jpg)
![Western Blot: Bestrophin 1 Antibody (E6-6) - BSA Free [NB300-164] - Detection of Bestrophin (68 kDa) from human RPE cell lysate.](http://images.novusbio.com/fullsize/Bestrophin-1-Antibody-E6-6-BSA-Free-Western-Blot-NB300-164-img0003.jpg)
![Immunohistochemistry-Paraffin: Bestrophin 1 Antibody (E6-6) - BSA Free [NB300-164] - Bestrophin 1 was detected in immersion fixed paraffin-embedded sections of human brain using Mouse Anti-Human Bestrophin 1 (E6-6) Monoclonal Antibody (Catalog # NB300-164) at 1:300 for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm in neurons.](http://images.novusbio.com/fullsize/Bestrophin-1-Antibody-E6-6-BSA-Free-Immunohistochemistry-Paraffin-NB300-164-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Bestrophin 1 Antibody (E6-6) - BSA Free [NB300-164] - Immunoblotting showing the expression of RPE-specific proteins BEST1 (NB300-164), RPE65 (NB100-355), CRALBP, and the loading control beta-Actin in iPSC-RPE cells. Two gels/blots in the same panel were prepared from the same cell lysate of each PSC-RPE to detect BEST1 + beta-Actin, and RPE65 + CRALBP, respectively. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34061021/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Bestrophin-1-Antibody-E6-6-BSA-Free-Knockout-Validated-NB300-164-img0009.jpg)
![Dual RNAscope ISH-IHC: Bestrophin 1 Antibody (E6-6) - BSA Free [NB300-164] - Formalin-fixed paraffin-embedded tissue sections of human duodenum were probed for Bestrophin 1 mRNA (ACD RNAScope Probe, catalog # 433181; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using mouse anti-human (Novus Biologicals catalog # NB300-164) at 0.3ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). IHC signal is confined to cytoplasm.](http://images.novusbio.com/fullsize/Bestrophin-1-Antibody-E6-6-BSA-Free-Dual-RNAscope-ISH-IHC-NB300-164-img0006.jpg)
![Western Blot: Bestrophin 1 Antibody (E6-6) - BSA Free [NB300-164] - (a) Schematic drawing showing localization of the different mutations (black diamonds) tested in our study (modified from [12]); (b) Western blot analysis of the normal & mutant human Best1 protein in transiently transfected MDCK cells. Best1 proteins are detectable as a 68 kDa band in all transfected cells, but not in non-transfected controls (MDCK lane). Actin bands are shown to indicate equal loading of cell lysates. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23880862), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-164_mouse-monoclonal-bestrophin-1-antibody-e6-6-31020241534336.jpg)
![Immunocytochemistry/ Immunofluorescence: Bestrophin 1 Antibody (E6-6) - BSA Free [NB300-164] - Subcellular localization of WT & mutant BEST1 in iPSC-RPEs. Confocal images showing the co-staining of BEST1, Collagen IV & Hoechst in iPSC-RPEs derived from a WT donor or patients. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31836750), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-164_mouse-monoclonal-bestrophin-1-antibody-e6-6-310202415363746.jpg)
![Western Blot: Bestrophin 1 Antibody (E6-6) - BSA Free [NB300-164] - (a) HPRT & BEST1 mRNAs are expressed in MDCK & RPE-J cells. M–100 bp ladder, N–negative control; (b) Quantification of BEST1 expression levels between MDCK & RPE-J cells using quantitative Real-Time PCR. Fold change variation in BEST1 expression levels is reported as 2^-delta delta Ct value, the reference mRNA being HPRT (mean ± SEM., n = 2); (c) Western blot analysis—Best1 protein is not synthesized by RPE-J or MDCK cells. After transfection, MDCK produce human Best1 at 68 kDa. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23880862), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-164_mouse-monoclonal-bestrophin-1-antibody-e6-6-310202415284544.jpg)
![Immunocytochemistry/ Immunofluorescence: Bestrophin 1 Antibody (E6-6) - BSA Free [NB300-164] - Characterization of WT iPSC & iPSC-RPE.(A) Phase picture of established WT iPSC line before differentiation. Scale bar, 400 μm. (B) Immunocytofluorescence images of pluripotency markers in established iPSC. Scale bar, 200 μm. (C) Confocal images showing plasma membrane localization of BEST1. Scale bar, 10 μm. (D) Comparison of current amplitudes in iPSC-RPEs from two BEST1 WT donors. Bar chart showing the steady-state current amplitudes at 0 [Ca2+]i, 1.2 μM [Ca2+]i, & 1.2 μM [Ca2+]i + 100 μM NFA in RPEs from two distinct BEST1 WT human donors; n = 5–6. ∗$p<0.05 compared to current amplitudes at 1.2 μM [Ca2+]i from donor #1 & #2, respectively, using two-tailed unpaired Student t test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29063836), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-164_mouse-monoclonal-bestrophin-1-antibody-e6-6-31020241535684.jpg)
![Western Blot: Bestrophin 1 Antibody (E6-6) - BSA Free [NB300-164] - CRISPR/Cas9-mediated gene silencing in combination with augmentation.(a) Augmented BEST1-GFP & endogenous BEST1 were detected by immunoblotting in hPSC-RPE cells. (b) Schematic of the baculovirus-based silencing (BVSi) vector. (c) Immunoblotting showing the knockdown of endogenous BEST1 expression with BVSi vectors & augmentation of wobble BEST1-mCherry in WT hPSC-RPE cells. (d) Immunoblotting showing the knockdown of endogenous BEST1 expression with BVSi 3–8 & augmentation of wobble BEST1-mCherry in hPSC-RPE cells carrying BEST1 gain-of-function mutations. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34061021), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-164_mouse-monoclonal-bestrophin-1-antibody-e6-6-31020241537190.jpg)
![Western Blot: Bestrophin 1 Antibody (E6-6) - BSA Free [NB300-164] - CRISPR/Cas9-mediated gene silencing in combination with augmentation.(a) Augmented BEST1-GFP & endogenous BEST1 were detected by immunoblotting in hPSC-RPE cells. (b) Schematic of the baculovirus-based silencing (BVSi) vector. (c) Immunoblotting showing the knockdown of endogenous BEST1 expression with BVSi vectors & augmentation of wobble BEST1-mCherry in WT hPSC-RPE cells. (d) Immunoblotting showing the knockdown of endogenous BEST1 expression with BVSi 3–8 & augmentation of wobble BEST1-mCherry in hPSC-RPE cells carrying BEST1 gain-of-function mutations. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34061021), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-164_mouse-monoclonal-bestrophin-1-antibody-e6-6-31020241539730.jpg)
![Western Blot: Bestrophin 1 Antibody (E6-6) - BSA Free [NB300-164] - Expression of RPE-specific marker proteins in hPSC-RPE & iPSC-RPE cells.(a–b) Immunoblotting showing the expression of RPE-specific proteins BEST1, RPE65, CRALBP, & the loading control beta -Actin in hPSC-RPE (a) & iPSC-RPE (b) cells. Two gels/blots in the same panel were prepared from the same cell lysate of each PSC-RPE to detect BEST1 + beta -Actin, & RPE65 + CRALBP, respectively. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34061021), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-164_mouse-monoclonal-bestrophin-1-antibody-e6-6-31020241535628.jpg)
![Immunocytochemistry/ Immunofluorescence: Bestrophin 1 Antibody (E6-6) - BSA Free [NB300-164] - Impact of BEST1 pathogenic variants in bestrophin-1 RPE localization. Cryosections obtained from the BD donors & an 88-year-old control were labeled with antibodies specific to bestrophin-1 (green), while cell nuclei have been labeled with TO-PRO-3 (blue). Bruch’s membrane is indicated by the hashed white line. Arrow = mislocalized apical RPE distribution of bestrophin-1; arrowheads = basolateral RPE distribution of bestrophin-1; double arrowheads = intracellular bestrophin-1. Scale bar = 40 μm (all images). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33154968), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-164_mouse-monoclonal-bestrophin-1-antibody-e6-6-310202415395959.jpg)
![Immunocytochemistry/ Immunofluorescence: Bestrophin 1 Antibody (E6-6) - BSA Free [NB300-164] - Subcellular localization of WT & mutant BEST1 in iPSC-RPEs. Confocal images showing the co-staining of BEST1, Collagen IV & Hoechst in iPSC-RPEs derived from a WT donor or patients. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31836750), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-164_mouse-monoclonal-bestrophin-1-antibody-e6-6-310202415175245.jpg)
![Immunocytochemistry/ Immunofluorescence: Bestrophin 1 Antibody (E6-6) - BSA Free [NB300-164] - (a) X-Z confocal single image scan of transiently transfected cells with different BEST1 cDNA constructs showing mislocalization of mutants Y85H, Q96R, L100R & Y227N. Cells were stained for Best1 (green), beta -catenin (red) & nuclei (blue). Scale bar = 10 μm; (b) Z-series confocal stack signals corresponding to each labeling were quantified. Curves indicate the pixel intensity of each section along the Z-axis for each cell (Best1, green; beta -catenin, red; nuclei, blue). The black vertical line indicates the Z-focal plane chosen as threshold for apical & basolateral domains separation. Basolateral & apical sides are as indicated. Horizontal axis represents μm distance & vertical axis shows pixel intensities; (c) Bar graph illustrating quantification of Best1 mutants distribution in the basolateral & apical domains of the cells compared with normal protein (mean ± SEM., n = 10, *p < 0.01, ***p < 0.0001). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23880862), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-164_mouse-monoclonal-bestrophin-1-antibody-e6-6-31020241621835.jpg)
![Western Blot: RPA2 [p Ser33] Antibody [NB100-544] - Whole cell lysate (50 ug) from HeLa cells treated with 100 uM etoposide for 16 hours (+) or mock treated (-) cells. Antibody: Affinity purified rabbit anti-Phospho RPA32 (S33) antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower panel: Rabbit anti-RPS3 antibody.](http://images.novusbio.com/fullsize/RPA2-[p-Ser33]-Antibody-Western-Blot-NB100-544-img0005.jpg "Western Blot: RPA2 [p Ser33] Antibody [NB100-544] - Whole cell lysate (50 ug) from HeLa cells treated with 100 uM etoposide for 16 hours (+) or mock treated (-) cells. Antibody: Affinity purified rabbit anti-Phospho RPA32 (S33) antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower panel: Rabbit anti-RPS3 antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: RPA2 [p Ser33] Antibody [NB100-544] - Active DNA end processing in late micronuclei compartments. Representative images (Upper panel) and quantification of RPA signal in MN compartments (Lower left) or RPA foci in nuclei (lower right) of post-IR OVCAR-8 cells after indicated treatments. Pro-resection genes (MRE11, BRCA1 and WDR70) were knockdown by specific siRNA. Percentage of positive RPA2-pS33 MN population was calculated after 36 h of IR treatment Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/s12885-018-4347-0), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RPA2-[p-Ser33]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-544-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: RPA2 [p Ser33] Antibody [NB100-544] - L.36 PDAC cells were treated two hours using 5 mM hydroxyurea to induce RPA32 Ser33 phosphorylation. The antibody produces clear nuclear staining of treated cells. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/RPA2-[p-Ser33]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-544-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: RPA2 [p Ser33] Antibody [NB100-544] - Active DNA end processing in late micronuclei compartments. Detection and quantification of RPA2 phosphorylation (pSerine 33) and ssDNA by specific antibodies in MN compartments of OVCAR-8 cells 36 h after 2-Gy irradiation. Note the nuclear signals have reduced while those in MN were intense. n = 3 biological repeats. Error bars = s.d. **P < 0.01; ***P < 0.005 (t-test). Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/s12885-018-4347-0), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RPA2-[p-Ser33]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-544-img0003.jpg)
![Western Blot: EB2 Antibody (1F3) [NBP2-00714] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EB2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EB2.](http://images.novusbio.com/fullsize/EB2-Antibody-1F3-Western-Blot-NBP2-00714-img0004.jpg "Western Blot: EB2 Antibody (1F3) [NBP2-00714] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EB2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EB2.")
![Immunocytochemistry/Immunofluorescence: EB2 Antibody (1F3) [NBP2-00714] Staining of COS7 cells transiently transfected by pCMV6-ENTRY EB2.](http://images.novusbio.com/fullsize/EB2-Antibody-1F3-Immunocytochemistry-Immunofluorescence-NBP2-00714-img0009.jpg)
![Immunohistochemistry-Paraffin: EB2 Antibody (1F3) [NBP2-00714] - Staining of paraffin-embedded Human pancreas tissue using anti-EB2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/EB2-Antibody-1F3-Immunohistochemistry-Paraffin-NBP2-00714-img0008.jpg)
![Flow Cytometry: EB2 Antibody (1F3) [NBP2-00714] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-EB2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/EB2-Antibody-1F3-Flow-Cytometry-NBP2-00714-img0003.jpg)
![Immunohistochemistry-Paraffin: EB2 Antibody (1F3) [NBP2-00714] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-EB2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/EB2-Antibody-1F3-Immunohistochemistry-Paraffin-NBP2-00714-img0005.jpg)
![Immunohistochemistry-Paraffin: EB2 Antibody (1F3) [NBP2-00714] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-EB2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/EB2-Antibody-1F3-Immunohistochemistry-Paraffin-NBP2-00714-img0006.jpg)
![Immunohistochemistry-Paraffin: EB2 Antibody (1F3) [NBP2-00714] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-EB2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/EB2-Antibody-1F3-Immunohistochemistry-Paraffin-NBP2-00714-img0007.jpg)
![Flow Cytometry: EB2 Antibody (1F3) [NBP2-00714] - Analysis of Hela cells, using anti-EB2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/EB2-Antibody-1F3-Flow-Cytometry-NBP2-00714-img0001.jpg)
![Flow Cytometry: EB2 Antibody (1F3) [NBP2-00714] - Analysis of Jurkat cells, using anti-EB2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/EB2-Antibody-1F3-Flow-Cytometry-NBP2-00714-img0002.jpg)