| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33402-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-17658-25ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1101AF405 | |||
| Alexa Fluor 488 | NB21-1101AF488 | |||
| Alexa Fluor 647 | NB21-1101AF647 | |||
| Alexa Fluor 700 | NB21-1101AF700 | |||
| Biotin | NB21-1101B | |||
| DyLight 405 | NB21-1101V | |||
| DyLight 488 | NB21-1101G | |||
| DyLight 550 | NB21-1101R | |||
| DyLight 650 | NB21-1101C | |||
| FITC | NB21-1101F | |||
| HRP | NB21-1101H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1101SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26021 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38032-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-37992-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-37994-20ul | |||
![Immunohistochemistry-Paraffin: Histone H1.1 Antibody [NBP2-33402] - Staining of human liver shows strong nuclear positivity in hepatocytes.](http://images.novusbio.com/fullsize/Histone-H1.1-Antibody-Immunohistochemistry-NBP2-33402-img0001.jpg "Immunohistochemistry-Paraffin: Histone H1.1 Antibody [NBP2-33402] - Staining of human liver shows strong nuclear positivity in hepatocytes.")
![Western Blot: Histone H1.1 Antibody [NBP3-12893] - HeLa whole cell and nuclear extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Histone H1.1 antibody (NBP3-12893) diluted at 1:1000.](http://images.novusbio.com/fullsize/Histone-H1.1-Antibody-Western-Blot-NBP3-12893-img0001.jpg "Western Blot: Histone H1.1 Antibody [NBP3-12893] - HeLa whole cell and nuclear extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Histone H1.1 antibody (NBP3-12893) diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Histone H1 Antibody [NBP3-17658] - Staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/Histone-H1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-17658-img0003.jpg "Immunocytochemistry/Immunofluorescence: Histone H1 Antibody [NBP3-17658] - Staining of human cell line U-2 OS shows localization to nucleoplasm.")
![Immunohistochemistry-Paraffin: Histone H1 Antibody [NBP3-17658] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Histone-H1-Antibody-Immunohistochemistry-Paraffin-NBP3-17658-img0001.jpg)
![Dot Blot: Histone H3 [p Thr11] Antibody [NB21-1101] - Analysis of H3pT11 with the peptides stated above in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr11]-Antibody-Dot-Blot-NB21-1101-img0003.jpg "Dot Blot: Histone H3 [p Thr11] Antibody [NB21-1101] - Analysis of H3pT11 with the peptides stated above in picomoles of peptide.")
![Western Blot: Histone H3 [p Thr11] Antibody [NB21-1101] - Analysis of H3pT11 in HeLa histone preps. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr11]-Antibody-Western-Blot-NB21-1101-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [p Thr11] Antibody [NB21-1101] - Staining of H3pT11 in HeLa cells using FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr11]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1101-img0006.jpg)
![Western Blot: Histone H3 [p Thr11] Antibody [NBP2-59274] - Western blot was performed on whole cell extracts from untreated HeLa cells (25 ug, lane 1), on histone extracts from HeLa cells treated with colcemid (15 ug, lane 2), and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3T11p . The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr11]-Antibody-Western-Blot-NBP2-59274-img0001.jpg "Western Blot: Histone H3 [p Thr11] Antibody [NBP2-59274] - Western blot was performed on whole cell extracts from untreated HeLa cells (25 ug, lane 1), on histone extracts from HeLa cells treated with colcemid (15 ug, lane 2), and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3T11p . The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [p Thr11] Antibody [NBP2-59274] - ChIP assays were performed using human HeLa cells, treated with colcemid, the antibody against H3T11p and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 0.5, 1, 2 and 5 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers for the EIF4A2 and GAPDH promoters, used as positive controls, and for the coding region of the inactive MYT1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr11]-Antibody-Chromatin-Immunoprecipitation-NBP2-59274-img0004.jpg)
![Dot Blot: Histone H3 [p Thr11] Antibody [NBP2-59274] - To test the cross reactivity of the antibody against H3T11p, a Dot Blot analysis was performed with peptides containing other histone phosphorylations and the unmodified H3T11. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr11]-Antibody-Dot-Blot-NBP2-59274-img0002.jpg)
![ELISA: Histone H3 [p Thr11] Antibody [NBP2-59274] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H3T11p in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:25,700.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr11]-Antibody-ELISA-NBP2-59274-img0003.jpg)
![Immunofluorescence: Histone H3 [p Thr11] Antibody [NBP2-59274] - HeLa cells were treated with colcemid and stained with the antibody against H3T11p and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3T11p antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr11]-Antibody-Immunofluorescence-NBP2-59274-img0005.jpg)
![Western Blot: Histone H3 [p Thr11] Antibody (RM164) [NBP2-61521] - Western Blot of acid extracts of HeLa cells treated or non-treated with Nocodazole using NBP2-61521 at 0.5 ug/ml. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-p-Thr11-Antibody-RM164-Western-Blot-NBP2-61521-img0002.jpg "Western Blot: Histone H3 [p Thr11] Antibody (RM164) [NBP2-61521] - Western Blot of acid extracts of HeLa cells treated or non-treated with Nocodazole using NBP2-61521 at 0.5 ug/ml. Observed molecular weight is ~16 kDa.")
![Western Blot: H4 Clustered Histone 1 [Sym-dimethyl Arg3] Antibody [NBP3-38032] - Western blot analysis of various lysates, using H4 Clustered Histone 1 Rabbit pAb at 1:400 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-38032_rabbit-h4-clustered-histone-1-sym-dimethyl-arg-3-pab-912202415143634.jpg "Western Blot: H4 Clustered Histone 1 [Sym-dimethyl Arg3] Antibody [NBP3-38032] - Western blot analysis of various lysates, using H4 Clustered Histone 1 Rabbit pAb at 1:400 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.")
![Western Blot: H4 Clustered Histone 1 [Sym-dimethyl Arg3] Antibody [NBP3-38032] - Western blot analysis of various lysates, using H4 Clustered Histone 1 Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-38032_rabbit-h4-clustered-histone-1-sym-dimethyl-arg-3-pab-912202415133497.jpg)
![Immunocytochemistry/ Immunofluorescence: H4 Clustered Histone 1 [Sym-dimethyl Arg3] Antibody [NBP3-38032] - Immunofluorescence analysis of 293T cells using Symmetric DiMethyl-Histone H4-R3 Rabbit pAb (A3159). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38032_rabbit-h4-clustered-histone-1-sym-dimethyl-arg-3-pab-912202415171184.jpg)
![Dot Blot: H4 Clustered Histone 1 [Sym-dimethyl Arg3] Antibody [NBP3-38032] - Dot-blot analysis of all sorts of methylation peptides using H4 Clustered Histone 1 antibody.](http://images.novusbio.com/fullsize/nbp3-38032_rabbit-h4-clustered-histone-1-sym-dimethyl-arg-3-pab-912202414213794.jpg)
![Immunohistochemistry: H4 Clustered Histone 1 [Sym-dimethyl Arg3] Antibody [NBP3-38032] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using H4 Clustered Histone 1 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38032_rabbit-h4-clustered-histone-1-sym-dimethyl-arg-3-pab-31120251704739.jpg)
![Immunohistochemistry: H4 Clustered Histone 1 [Sym-dimethyl Arg3] Antibody [NBP3-38032] - Immunohistochemistry analysis of paraffin-embedded Mouse intestin tissue using H4 Clustered Histone 1 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38032_rabbit-h4-clustered-histone-1-sym-dimethyl-arg-3-pab-31120251744466.jpg)
![Immunohistochemistry: H4 Clustered Histone 1 [Sym-dimethyl Arg3] Antibody [NBP3-38032] - Immunohistochemistry analysis of paraffin-embedded Human liver tissue using H4 Clustered Histone 1 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38032_rabbit-h4-clustered-histone-1-sym-dimethyl-arg-3-pab-31120251659368.jpg)
![Immunohistochemistry: H4 Clustered Histone 1 [Sym-dimethyl Arg3] Antibody [NBP3-38032] - Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using H4 Clustered Histone 1 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38032_rabbit-h4-clustered-histone-1-sym-dimethyl-arg-3-pab-3112025173115.jpg)
![Immunohistochemistry: H4 Clustered Histone 1 [Sym-dimethyl Arg3] Antibody [NBP3-38032] - Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using H4 Clustered Histone 1 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38032_rabbit-h4-clustered-histone-1-sym-dimethyl-arg-3-pab-3112025173112.jpg)
![Immunohistochemistry: H4 Clustered Histone 1 [Sym-dimethyl Arg3] Antibody [NBP3-38032] - Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using H4 Clustered Histone 1 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38032_rabbit-h4-clustered-histone-1-sym-dimethyl-arg-3-pab-311202516593621.jpg)
![Immunohistochemistry: H4 Clustered Histone 1 [Sym-dimethyl Arg3] Antibody [NBP3-38032] - Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using H4 Clustered Histone 1 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38032_rabbit-h4-clustered-histone-1-sym-dimethyl-arg-3-pab-31120251704719.jpg)
![Immunohistochemistry: H4 Clustered Histone 1 [Sym-dimethyl Arg3] Antibody [NBP3-38032] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using H4 Clustered Histone 1 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38032_rabbit-h4-clustered-histone-1-sym-dimethyl-arg-3-pab-3112025173135.jpg)
![Immunohistochemistry: H4 Clustered Histone 1 [Sym-dimethyl Arg3] Antibody [NBP3-38032] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using H4 Clustered Histone 1 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38032_rabbit-h4-clustered-histone-1-sym-dimethyl-arg-3-pab-31120251744497.jpg)
![Immunohistochemistry: H4 Clustered Histone 1 [Sym-dimethyl Arg3] Antibody [NBP3-38032] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using H4 Clustered Histone 1 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38032_rabbit-h4-clustered-histone-1-sym-dimethyl-arg-3-pab-311202517702.jpg)
![Immunohistochemistry: H4 Clustered Histone 1 [Sym-dimethyl Arg3] Antibody [NBP3-38032] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using H4 Clustered Histone 1 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38032_rabbit-h4-clustered-histone-1-sym-dimethyl-arg-3-pab-31120251744441.jpg)
![Dot Blot: H4 Clustered Histone 1 [Monomethyl Lys20] Antibody [NBP3-37992] - Dot-blot analysis of all sorts of methylation peptides using H4 Clustered Histone 1 antibody.](http://images.novusbio.com/fullsize/nbp3-37992_rabbit-h4-clustered-histone-1-monomethyl-lys-20-pab-912202415175262.jpg "Dot Blot: H4 Clustered Histone 1 [Monomethyl Lys20] Antibody [NBP3-37992] - Dot-blot analysis of all sorts of methylation peptides using H4 Clustered Histone 1 antibody.")
![Western Blot: H4 Clustered Histone 1 [Monomethyl Lys20] Antibody [NBP3-37992] - Western blot analysis of various lysates using H4 Clustered Histone 1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-37992_rabbit-h4-clustered-histone-1-monomethyl-lys-20-pab-912202414154391.jpg)
![Immunocytochemistry/ Immunofluorescence: H4 Clustered Histone 1 [Monomethyl Lys20] Antibody [NBP3-37992] - Immunofluorescence analysis of 293T cells using H4 Clustered Histone 1 Rabbit pAb. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-37992_rabbit-h4-clustered-histone-1-monomethyl-lys-20-pab-912202414201393.jpg)
![Immunohistochemistry: H4 Clustered Histone 1 [Monomethyl Lys20] Antibody [NBP3-37992] - Immunohistochemistry analysis of paraffin-embedded Mouse testis using H4 Clustered Histone 1 Rabbit pAb at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-37992_rabbit-h4-clustered-histone-1-monomethyl-lys-20-pab-912202415111815.jpg)
![Immunohistochemistry: H4 Clustered Histone 1 [Monomethyl Lys20] Antibody [NBP3-37992] - Immunohistochemistry analysis of paraffin-embedded Rat testis using H4 Clustered Histone 1 Rabbit pAb at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-37992_rabbit-h4-clustered-histone-1-monomethyl-lys-20-pab-912202415111835.jpg)
![Immunohistochemistry: H4 Clustered Histone 1 [Monomethyl Lys20] Antibody [NBP3-37992] - Immunohistochemistry analysis of paraffin-embedded Human colon using H4 Clustered Histone 1 Rabbit pAb at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-37992_rabbit-h4-clustered-histone-1-monomethyl-lys-20-pab-912202414201331.jpg)
![Western Blot: H4 Clustered Histone 1 [Monomethyl Lys20] Antibody [NBP3-37992] - Western blot analysis of various lysates using H4 Clustered Histone 1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-37992_rabbit-h4-clustered-histone-1-monomethyl-lys-20-pab-912202415111885.jpg)
![Immunohistochemistry: H4 Clustered Histone 1 [Trimethyl Lys20] Antibody [NBP3-37994] - Immunohistochemistry analysis of paraffin-embedded Mouse testis using H4 Clustered Histone 1 Rabbit pAb at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-37994_rabbit-h4-clustered-histone-1-trimethyl-lys-20-pab-91220241411284.jpg "Immunohistochemistry: H4 Clustered Histone 1 [Trimethyl Lys20] Antibody [NBP3-37994] - Immunohistochemistry analysis of paraffin-embedded Mouse testis using H4 Clustered Histone 1 Rabbit pAb at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Western Blot: H4 Clustered Histone 1 [Trimethyl Lys20] Antibody [NBP3-37994] - Western blot analysis of various lysates, using H4 Clustered Histone 1 Rabbit pAb at 1:400 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-37994_rabbit-h4-clustered-histone-1-trimethyl-lys-20-pab-912202414213738.jpg)
![Dot Blot: H4 Clustered Histone 1 [Trimethyl Lys20] Antibody [NBP3-37994] - Dot-blot analysis of all sorts of methylation peptides using H4 Clustered Histone 1 antibody.](http://images.novusbio.com/fullsize/nbp3-37994_rabbit-h4-clustered-histone-1-trimethyl-lys-20-pab-912202415143656.jpg)
![Immunocytochemistry/ Immunofluorescence: H4 Clustered Histone 1 [Trimethyl Lys20] Antibody [NBP3-37994] - Immunofluorescence analysis of 293T cells using H4 Clustered Histone 1 Rabbit pAb. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-37994_rabbit-h4-clustered-histone-1-trimethyl-lys-20-pab-912202415175285.jpg)
![Immunohistochemistry: H4 Clustered Histone 1 [Trimethyl Lys20] Antibody [NBP3-37994] - Immunohistochemistry analysis of paraffin-embedded Rat testis using H4 Clustered Histone 1 Rabbit pAb at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-37994_rabbit-h4-clustered-histone-1-trimethyl-lys-20-pab-9122024159529.jpg)