| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-30498-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74924 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-82050 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-82050 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74924 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74924 | |||
![Western Blot: ZFYVE21 Antibody [H00079038-B01P] - Membrane attack complex-induced and endosome-dependent stabilization of ZFYVE21. Human umbilical vein endothelial cells (HUVECs) were treated with panel reactive antibody (PRA) sera (25% v/v in gelatin veronal buffer) (a). HUVECs were treated with MG132 (25uM). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-019-10041-2), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZFYVE21-Antibody-Western-Blot-H00079038-B01P-img0005.jpg "Western Blot: ZFYVE21 Antibody [H00079038-B01P] - Membrane attack complex-induced and endosome-dependent stabilization of ZFYVE21. Human umbilical vein endothelial cells (HUVECs) were treated with panel reactive antibody (PRA) sera (25% v/v in gelatin veronal buffer) (a). HUVECs were treated with MG132 (25uM). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-019-10041-2), licensed under a CC-BY license.")
![Western Blot: ZFYVE21 Antibody [H00079038-B01P] - Analysis of ZFYVE21 expression in transfected 293T cell line by ZFYVE21 polyclonal antibody. Lane 1: ZFYVE21 transfected lysate(25.74 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/ZFYVE21-Antibody-Western-Blot-H00079038-B01P-img0002.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: ZFYVE21 Antibody [H00079038-B01P] - Correlations of ZFYVE21 and complement in patient tissues. Western blots of human umbilical vein endothelial cells treated with either panel reactive antibody (30min, 25% v/v in gelatin veronal buffer) or LIGHT (12h, 50ng/mL in EGM2 media, a). Kidney biopsies from renal transplant patients (n=12) were stained as indicated. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-019-10041-2), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZFYVE21-Antibody-Western-Blot-H00079038-B01P-img0003.jpg)
![Western Blot: ZFYVE21 Antibody [H00079038-B01P] - Recombinant FLAG-tagged ZFYVE21 (1ug) and/or recombinant SMURF2 (1ug) protein was incubated for 30min at room temperature and analyzed by western blot. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-019-10041-2), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZFYVE21-Antibody-Western-Blot-H00079038-B01P-img0004.jpg)
![Western Blot: ZFYVE21 Antibody [H00079038-B01P] - Correlations of ZFYVE21 & complement in patient tissues. Western blots of human umbilical vein endothelial cells treated with either panel reactive antibody (30 min, 25% v/v in gelatin veronal buffer) or LIGHT (12 h, 50 ng/mL in EGM2 media, a). Kidney biopsies from renal transplant patients (n = 12) were stained as indicated (b). Synovial biopsies from osteoarthritis (n = 11), low-titer rheumatoid factor antibody rheumatoid arthritis (RF RA; n = 8), & high-titer RF RA (n = 8) were stained as indicated (c–e) & spatial correlations between ZFVYE21 or nuclear factor-inducing kinase (NIK) with endothelial cells (Ulex, f) & membrane attack complex (polyC9, g) were assessed using the computer software (scale bar: 200 μm). ****p < 1 × 10−5, Student’s t test. CD45 abundance was assessed (h) & correlated with NIK staining (i) via blinded scoring from three independent observers. *p < 0.05, **p < 0.001. Western blotting was repeated three times. Analysis of variance statistical analyses were performed. Representative data shown *p < 0.05, **p < 0.001 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31113953), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00079038-b01p_mouse-polyclonal-zfyve21-antibody-31020241548367.jpg)
![Western Blot: ZFYVE21 Antibody [H00079038-B01P] - SMURF2-dependent degradation of vesicular PTEN. PTEN co-immunoprecipitations (co-IPs) were performed on human umbilical vein endothelial cells (HUVECs) treated for 30 min with panel reactive antibody (PRA) sera & blotted as indicated (a). Rab5 co-IPs were performed on PRA-treated HUVECs transfected with control or SMURF2 siRNA (b). Western blot analysis of whole-cell lysates of HUVECs transiently transfected with SMURF2 WT, SMURF2 DN, control siRNA, & SMURF2 siRNA (c). HUVECs transfected with control, SMURF2, or ZFYVE21 siRNA were treated with PRA & Rab5 co-IPs were performed (d). Recombinant FLAG-tagged ZFYVE21 (1 μg) and/or recombinant SMURF2 (1 μg) protein was incubated for 30 min at room temperature & analyzed by western blot (e). In vitro kinase assays were performed using various E2 ubiquitin ligases with SMURF2 protein (1 μg) as the E3 ubiquitin ligase & PTEN (1 μg) as substrate (f). In vitro kinase assays were performed using isolated SMURF2 WT or SMURF2 DN as E3 ubiquitin ligases, UbcH5c as the E2 ubiquitin ligase, & PTEN as substrate as indicated (g). HUVECs were pretreated with MG132 for 4 h or chloroquine for 30 min prior to PRA treatment at the indicated times prior to Rab5 co-IP & western blot analysis (h). Western blot analyses were repeated two to four times. Confocal microscopic studies analyzed ≥4 cells per group & was repeated three times. In vitro kinase assays were repeated twice. Representative data shown Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31113953), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00079038-b01p_mouse-polyclonal-zfyve21-antibody-310202415485117.jpg)
![Western Blot: ZFYVE21 Antibody [NBP2-82050] - Western blot analysis of ZFYVE21 in 3T3 cell tissue lysate with ZFYVE21 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/ZFYVE21-Antibody-Western-Blot-NBP2-82050-img0001.jpg "Western Blot: ZFYVE21 Antibody [NBP2-82050] - Western blot analysis of ZFYVE21 in 3T3 cell tissue lysate with ZFYVE21 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.")
![Immunocytochemistry/ Immunofluorescence: ZFYVE21 Antibody - BSA Free [NBP2-82050] - Immunofluorescence of ZFYVE21 in human kidney tissue with ZFYVE21 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-82050_rabbit-polyclonal-zfyve21-antibody-2711202420414546.jpg)
![Immunohistochemistry: ZFYVE21 Antibody [NBP2-30498] - Staining of human kidney shows cytoplasmic and extracellular positivity in glomeruli.](http://images.novusbio.com/fullsize/ZFYVE21-Antibody-Immunohistochemistry-NBP2-30498-img0001.jpg "Immunohistochemistry: ZFYVE21 Antibody [NBP2-30498] - Staining of human kidney shows cytoplasmic and extracellular positivity in glomeruli.")
![Western Blot: ZFYVE21 Antibody [NBP1-98434] - HCT15 Cell Lysate 1.0 ug/ml, Gel Concentration: 12%](http://images.novusbio.com/fullsize/ZFYVE21-Antibody-Western-Blot-NBP1-98434-img0002.jpg "Western Blot: ZFYVE21 Antibody [NBP1-98434] - HCT15 Cell Lysate 1.0 ug/ml, Gel Concentration: 12%")
![Western Blot: ZFYVE21 Antibody (2B12) [NBP2-46407] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZFYVE21.](http://images.novusbio.com/fullsize/ZFYVE21-Antibody-2B12-Western-Blot-NBP2-46407-img0001.jpg "Western Blot: ZFYVE21 Antibody (2B12) [NBP2-46407] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZFYVE21.")
![Western Blot: ZFYVE21 Overexpression Lysate (Adult Normal) [NBL1-18027] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ZFYVE21.](http://images.novusbio.com/fullsize/ZFYVE21-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-18027-img0002.jpg "Western Blot: ZFYVE21 Overexpression Lysate (Adult Normal) [NBL1-18027] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ZFYVE21.")