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![Western Blot: KAP1 Antibody [NBP2-32071] - Samples: Whole cell lysate (50 ug) from TCMK-1 cells treated with 100 uM EPE for 2 hours (+) or mock treated (-), NIH3T3, and Renca cells. Antibodies: Affinity purified rabbit anti-KAP-1 antibody NBP2-32071 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/KAP1-Antibody-Western-Blot-NBP2-32071-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: KAP1 Antibody [NBP2-32071] - Samples: Whole cell lysate (50 ug) from TCMK-1 cells treated with 100 uM EPE for 2 hours (+) or mock treated (-), NIH3T3, and Renca cells. Antibodies: Affinity purified rabbit anti-KAP-1 antibody NBP2-32071 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: KAP1 Antibody [NBP2-32071] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from TCMK-1 cells treated with 100 uM EPE for 2 hours (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-KAP-1 antibody NBP2-32071 used for IP at 6 ug/mg lysate. KAP-1 was also immunoprecipitated by rabbit anti-KAP-1 antibody NBP2-32071. For blotting immunoprecipitated KAP-1, NBP2-32071 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/KAP1-Antibody-Immunoprecipitation-NBP2-32071-img0004.jpg)
![Western Blot: KAP1 [p Ser824] Antibody [NBP2-32073] - Detection of mouse Phospho KAP-1 (S824) by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 cells treated with etoposide (+) or mock treated (-). Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) antibody NBP2-32073 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower panel: Western blot for total KAP-1 using a different affinity purified rabbit anti-KAP-1 antibody at 0.4 ug/ml with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/KAP1-[p-Ser824]-Antibody-Western-Blot-NBP2-32073-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: KAP1 [p Ser824] Antibody [NBP2-32073] - Detection of mouse Phospho KAP-1 (S824) by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 cells treated with etoposide (+) or mock treated (-). Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) antibody NBP2-32073 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower panel: Western blot for total KAP-1 using a different affinity purified rabbit anti-KAP-1 antibody at 0.4 ug/ml with an exposure time of 3 seconds.")
![<b>Independent Antibodies Validation. </b>Western Blot: KAP1 [p Ser824] Antibody [NBP2-32073] - Detection of mouse Phospho KAP-1 (S824) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from TCMK-1 cells treated with etoposide (+) and/or Lambda PP (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-KAP-1 antibody NBP2-32073 used for IP at 6 ug per reaction. For blotting immunoprecipitated Phospho KAP-1 (S824), NBP2-32073 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-KAP-1 antibody A304-145A.](http://images.novusbio.com/fullsize/KAP1-[p-Ser824]-Antibody-Immunoprecipitation-NBP2-32073-img0002.jpg)
![Western Blot: KAP1 [p Ser 824] Antibody (SR1057) [NBP3-21591] - Western blot analysis using NBP3-21591 in HeLa cell treated with etoposide.](http://images.novusbio.com/fullsize/nbp3-21591_rabbit-kap1-p-ser-824-mab-sr1057-57202317161890.jpg "Western Blot: KAP1 [p Ser 824] Antibody (SR1057) [NBP3-21591] - Western blot analysis using NBP3-21591 in HeLa cell treated with etoposide.")
![ELISA: Human KAP1 ELISA Kit (Colorimetric) [NBP3-18727] - Standard Reference Curve](http://images.novusbio.com/fullsize/Human-KAP1-ELISA-Kit-Colorimetric-ELISA-NBP3-18727-img0001.jpg "ELISA: Human KAP1 ELISA Kit (Colorimetric) [NBP3-18727] - Standard Reference Curve")
![SDS-Page: KAP1 Protein [NBP2-22889]](http://images.novusbio.com/fullsize/KAP1-Protein-SDS-Page-NBP2-22889-img0001.jpg "SDS-Page: KAP1 Protein [NBP2-22889]")