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![Western Blot: p53 [p Thr81] Antibody [NBP3-16955] - Western blot analysis of extracts of C6 cells, using p53antibody (NBP3-16955) at 1:500 dilution.C6 cells were treated by nocodazole (50 ng/ml) at 37C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/p53-[p-Thr81]-Antibody-Western-Blot-NBP3-16955-img0001.jpg "Western Blot: p53 [p Thr81] Antibody [NBP3-16955] - Western blot analysis of extracts of C6 cells, using p53antibody (NBP3-16955) at 1:500 dilution.C6 cells were treated by nocodazole (50 ng/ml) at 37C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Western Blot: p53 [p Ser33] Antibody [NBP3-13309] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 (phospho Ser33) antibody (NBP3-13309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/p53-[p-Ser33]-Antibody-Western-Blot-NBP3-13309-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: p53 [p Ser33] Antibody [NBP3-13309] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 (phospho Ser33) antibody (NBP3-13309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![ELISA: Rat p53 ELISA Kit (Chemiluminescence) [NBP2-75357] - Samples were spiked with high concentrations of Rat p53 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75357_rat-p53-elisa-kit-chemiluminescence-132202416121310.jpg "ELISA: Rat p53 ELISA Kit (Chemiluminescence) [NBP2-75357] - Samples were spiked with high concentrations of Rat p53 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat p53 ELISA Kit (Chemiluminescence) [NBP2-75357] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75357_rat-p53-elisa-kit-chemiluminescence-432024856773.jpg)
![ELISA: Mouse p53 ELISA Kit (Colorimetric) [NBP2-75358] - Samples were spiked with high concentrations of Mouse p53 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75358_mouse-p53-elisa-kit-colorimetric-13220241681497.jpg "ELISA: Mouse p53 ELISA Kit (Colorimetric) [NBP2-75358] - Samples were spiked with high concentrations of Mouse p53 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse p53 ELISA Kit (Colorimetric) [NBP2-75358] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75358_mouse-p53-elisa-kit-colorimetric-432024856785.jpg)
![ELISA: Mouse p53 ELISA Kit (Chemiluminescence) [NBP2-75356] - Samples were spiked with high concentrations of Mouse p53 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75356_mouse-p53-elisa-kit-chemiluminescence-132202416225231.jpg "ELISA: Mouse p53 ELISA Kit (Chemiluminescence) [NBP2-75356] - Samples were spiked with high concentrations of Mouse p53 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse p53 ELISA Kit (Chemiluminescence) [NBP2-75356] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75356_mouse-p53-elisa-kit-chemiluminescence-432024856786.jpg)
![ELISA: Human p53 ELISA Kit (Chemiluminescence) [NBP2-75355] - Samples were spiked with high concentrations of Human p53 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75355_human-p53-elisa-kit-chemiluminescence-132202416211363.jpg "ELISA: Human p53 ELISA Kit (Chemiluminescence) [NBP2-75355] - Samples were spiked with high concentrations of Human p53 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human p53 ELISA Kit (Chemiluminescence) [NBP2-75355] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75355_human-p53-elisa-kit-chemiluminescence-4320249133275.jpg)
![ELISA: Rat p53 ELISA Kit (Colorimetric) [NBP2-75359] - Samples were spiked with high concentrations of Rat p53 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75359_rat-p53-elisa-kit-colorimetric-132202416221043.jpg "ELISA: Rat p53 ELISA Kit (Colorimetric) [NBP2-75359] - Samples were spiked with high concentrations of Rat p53 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat p53 ELISA Kit (Colorimetric) [NBP2-75359] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75359_rat-p53-elisa-kit-colorimetric-432024973635.jpg)
![Western Blot: p53 Overexpression Lysate (Adult Normal) [NBP2-11392] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for p53.](http://images.novusbio.com/fullsize/p53-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11392-img0001.jpg "Western Blot: p53 Overexpression Lysate (Adult Normal) [NBP2-11392] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for p53.")
![Western Blot: p53 Overexpression Lysate (Adult Normal) [NBL1-17203] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for p53.](http://images.novusbio.com/fullsize/p53-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17203-img0002.jpg "Western Blot: p53 Overexpression Lysate (Adult Normal) [NBL1-17203] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for p53.")
![Western Blot: p53 [p Ser9] Antibody (10U5R6) [NBP3-16909] - Western blot analysis of extracts of NIH/3T3 cells, using p53 Rabbit mAb (NBP3-16909) at 1:1000 dilution.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/p53-p-Ser9-Antibody-10U5R6-Western-Blot-NBP3-16909-img0001.jpg "Western Blot: p53 [p Ser9] Antibody (10U5R6) [NBP3-16909] - Western blot analysis of extracts of NIH/3T3 cells, using p53 Rabbit mAb (NBP3-16909) at 1:1000 dilution.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 3min.")
![Western Blot: p53 [p Ser 376] Antibody (SR1943) [NBP3-27814] - Western blot analysis of HEK293 cell lysates treated with Calyculin A and Okadaic Acid.](http://images.novusbio.com/fullsize/nbp3-27814_rabbit-p53-p-ser-376-mab-sr1943-75202410133425.jpg "Western Blot: p53 [p Ser 376] Antibody (SR1943) [NBP3-27814] - Western blot analysis of HEK293 cell lysates treated with Calyculin A and Okadaic Acid.")