TEK Products By Gene Name

313-TI) was serially diluted and captured by Mouse Anti-Human Tie‑2 Monoclonal Antibody (Catalog # MAB3132) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Tie‑2 Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_lightbox"> 313-TI) was serially diluted and captured by Mouse Anti-Human Tie‑2 Monoclonal Antibody (Catalog # MAB3132) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Tie‑2 Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" alt="Recombinant Human Tie‑2 Fc Chimera (Catalog # 313-TI) was serially diluted and captured by Mouse Anti-Human Tie‑2 Monoclonal Antibody (Catalog # MAB3132) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Tie‑2 Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_thumb" />

623-AN) binds to immobilized Recombinant Zebrafish Tie-2 Fc Chimera (Catalog # 928-T2) coated at 4 µg/mL (100 µL/well) in a dose-dependent manner (orange line). Binding is blocked (green line) by increasing concentrations of Mouse Anti-Zebrafish Tie-2 Monoclonal Antibody (Catalog # MAB928). At 0.5 µg/mL, this antibody will block >90% of the binding." class="big_lightbox"> 623-AN) binds to immobilized Recombinant Zebrafish Tie-2 Fc Chimera (Catalog # 928-T2) coated at 4 µg/mL (100 µL/well) in a dose-dependent manner (orange line). Binding is blocked (green line) by increasing concentrations of Mouse Anti-Zebrafish Tie-2 Monoclonal Antibody (Catalog # MAB928). At 0.5 µg/mL, this antibody will block >90% of the binding." alt="In a functional ELISA, Recombinant Human Angiopoietin-2 (Catalog # 623-AN) binds to immobilized Recombinant Zebrafish Tie-2 Fc Chimera (Catalog # 928-T2) coated at 4 µg/mL (100 µL/well) in a dose-dependent manner (orange line). Binding is blocked (green line) by increasing concentrations of Mouse Anti-Zebrafish Tie-2 Monoclonal Antibody (Catalog # MAB928). At 0.5 µg/mL, this antibody will block >90% of the binding." class="big_thumb" />

BAM1676) to detect phospho-Tie-2. Bands were visualized with Streptavidin-HRP (Cat # DY998) followed by chemiluminescent detection using WesternGlo^TM Chemiluminescent Detection Substrate (Catalog # AR004)." class="big_lightbox"> BAM1676) to detect phospho-Tie-2. Bands were visualized with Streptavidin-HRP (Cat # DY998) followed by chemiluminescent detection using WesternGlo^TM Chemiluminescent Detection Substrate (Catalog # AR004)." alt="Human Tie-2 transfected Baf/3 cells (Baf/3-hTie-2) were treated with 600 ng/mL recombinant human Ang-1 for seven minutes to induce tyrosine phosphorylation of Tie-2. Serial dilutions of lysates were analyzed by (A) IP-Western blot and (B) this ELISA. IPs were done using an anti-Tie-2 monoclonal antibody and goat anti-mouse agarose. Immunoblots were incubated with a biotinylated anti-phosphotyrosine monoclonal antibody (Catalog # BAM1676) to detect phospho-Tie-2. Bands were visualized with Streptavidin-HRP (Cat # DY998) followed by chemiluminescent detection using WesternGlo^TM Chemiluminescent Detection Substrate (Catalog # AR004)." class="big_thumb" />

BAF313). Human Phospho-Tie-2 can be detected in this ELISA by using approximately 4 times less lysate than is needed for a conventional IP-Western blot." class="big_lightbox"> BAF313). Human Phospho-Tie-2 can be detected in this ELISA by using approximately 4 times less lysate than is needed for a conventional IP-Western blot." class="big_thumb" />

313-TI), human Tie-1 (Catalog #619-TI), human VEGF R2 (Catalog #357-KD) or human VEGF R3 (Catalog #321-FL) were added to 50 μg lysate and analyzed using this ELISA. Competition was observed only with recombinant Tie-2. " class="big_lightbox"> 313-TI), human Tie-1 (Catalog #619-TI), human VEGF R2 (Catalog #357-KD) or human VEGF R3 (Catalog #321-FL) were added to 50 μg lysate and analyzed using this ELISA. Competition was observed only with recombinant Tie-2. " class="big_thumb" />