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SPINK1 Products By Gene Name

16 Results for "SPINK1"

NBP1-87314PEP

SPINK1 Recombinant Protein Antigen

0.1 ml

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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MAB7496

SPINK1 Antibody (839305) [Unconjugated]

(4 Publications)

100 μg

MAB7496

Western blot shows lysates of human pancreas tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human SPINK1 Monoclonal Antibody (Catalog # MAB7496) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SPINK1 at approximately 6 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of human pancreas tissue, loaded at 0.5 mg/mL. A specific band was detected for SPINK1 at approximately 6 kDa (as indicated) using 100 µg/mL of Mouse Anti-Human SPINK1 Monoclonal Antibody (Catalog # MAB7496). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Androgen signaling negatively regulates SPINK1 expression in prostate cancer.a Immunoblot for SPINK1 in 22RV1 cells stimulated with R1881 (10 nM) (top). QPCR data showing relative expression of SPINK1&KLK3 in the same cells (bottom). Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31959826), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reprogramming factor SOX2&AR transcriptional co-repressor REST modulate SPINK1 expression.a Schematic showing SOX2 binding elements (S1, S2,&S3) on the SPINK1 promoter (top). ChIP-qPCR data for SOX2 occupancy on the SPINK1 promoter in wildtype LNCaP&LNCaP-AI cells (androgen-deprived for 15 days) (bottom). b Same as in a, except 22RV1 cells. c ChIP-qPCR data for RNA Pol-II binding on the SPINK1 promoter using cells as a. d Same as c, except 22RV1 cells. e Immunoblot for SOX2&SPINK1 in siRNA mediated SOX2-silenced LNCaP-AI&control cells. f Same as e, except 22RV1 cells.Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31959826), licensed under a CC-BY license. Not internally tested by R&D Systems.

Androgen signaling negatively regulates SPINK1 expression in prostate cancer. h Immunoblot showing SPINK1 expression in VCaP cells treated with enzalutamide (top). QPCR data showing relative expression of SPINK1, KLK3,&ERG (bottom). Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31959826), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reprogramming factor SOX2&AR transcriptional co-repressor REST modulate SPINK1 expression.a Schematic showing SOX2 binding elements (S1, S2,&S3) on the SPINK1 promoter (top). ChIP-qPCR data for SOX2 occupancy on the SPINK1 promoter in wildtype LNCaP&LNCaP-AI cells (androgen-deprived for 15 days) (bottom). b Same as in a, except 22RV1 cells. c ChIP-qPCR data for RNA Pol-II binding on the SPINK1 promoter using cells as a. d Same as c, except 22RV1 cells. e Immunoblot for SOX2&SPINK1 in siRNA mediated SOX2-silenced LNCaP-AI&control cells. f Same as e, except 22RV1 cells. g QPCR data showing relative expression of SOX2&SPINK1 upon transient SOX2 overexpression in LNCaP cells (left). Immunoblot for SOX2&SPINK1 expression(right). Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31959826), licensed under a CC-BY license. Not internally tested by R&D Systems.

Androgen signaling negatively regulates SPINK1 expression in prostate cancer. l Immunoblot showing SPINK1 expression in 22RV1 cells as indicated in k (top). QPCR data showing relative expression of SPINK1&KLK3 using same cells in k (bottom)Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31959826), licensed under a CC-BY license. Not internally tested by R&D Systems.

Androgen signaling negatively regulates SPINK1 expression in prostate cancer.a Immunoblot for SPINK1 in 22RV1 cells stimulated with R1881 (10 nM) (top). QPCR data showing relative expression of SPINK1&KLK3 in the same cells (bottom). b Immunostaining for SPINK1&AR in 22RV1 cells stimulated with R1881 (10 nM). c Same as b, except dot plot represents quantification for SPINK1 mean fluorescence intensity (MFI) per unit area shown as arbitrary units (AU). d Same as a, except VCaP cells used. Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31959826), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reprogramming factor SOX2&AR transcriptional co-repressor REST modulate SPINK1 expression.a Schematic showing SOX2 binding elements (S1, S2,&S3) on the SPINK1 promoter (top). ChIP-qPCR data for SOX2 occupancy on the SPINK1 promoter in wildtype LNCaP&LNCaP-AI cells (androgen-deprived for 15 days) (bottom). b Same as in a, except 22RV1 cells. c ChIP-qPCR data for RNA Pol-II binding on the SPINK1 promoter using cells as a. d Same as c, except 22RV1 cells. e Immunoblot for SOX2&SPINK1 in siRNA mediated SOX2-silenced LNCaP-AI&control cells. Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31959826), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reprogramming factor SOX2&AR transcriptional co-repressor REST modulate SPINK1 expression.k Immunoblot for the REST&SPINK1 levels in 22RV1 cells treated with Casein Kinase 1 inhibitor (iCK1) as indicated (top). QPCR data for relative SPINK1&SYP expression (bottom). Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31959826), licensed under a CC-BY license. Not internally tested by R&D Systems.

SPINK1 promotes EMT, stemness&chemoresistance in prostate cancer.a Immunoblot analysis for SPINK1, E-Cadherin&Vimentin levels in stable SPINK1-silenced (shSPINK1-1&shSPINK1-2)&control (shSCRM) 22RV1 cells. Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31959826), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB7496-SP

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NBP2-98602UV

SPINK1 Antibody [DyLight 350]

0.1 ml

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

DyLight 350

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-98602AF350
Alexa Fluor 405	NBP2-98602AF405
Alexa Fluor 488	NBP2-98602AF488
Alexa Fluor 532	NBP2-98602AF532
Alexa Fluor 594	NBP2-98602AF594
Alexa Fluor 647	NBP2-98602AF647
Alexa Fluor 700	NBP2-98602AF700
Alexa Fluor 750	NBP2-98602AF750
Allophycocyanin	NBP2-98602APC
Biotin	NBP2-98602B
CoraFluor 1	NBP2-98602CL1
DyLight 405	NBP2-98602V
DyLight 488	NBP2-98602G
DyLight 550	NBP2-98602R
DyLight 594	NBP2-98602DL594
DyLight 650	NBP2-98602C
DyLight 680	NBP2-98602FR
DyLight 755	NBP2-98602IR
FITC	NBP2-98602F
HRP	NBP2-98602H
Janelia Fluor 525	NBP2-98602JF525
Janelia Fluor 549	NBP2-98602JF549
Janelia Fluor 585	NBP2-98602JF585
Janelia Fluor 635	NBP2-98602JF635
Janelia Fluor 646	NBP2-98602JF646
Janelia Fluor 669	NBP2-98602JF669
PE	NBP2-98602PE
PerCP	NBP2-98602PCP
mFluor Violet 450 SE	NBP2-98602MFV450
mFluor Violet 500 SE	NBP2-98602MFV500
mFluor Violet 610 SE	NBP2-98602MFV610

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NBP2-98602APC

SPINK1 Antibody [Allophycocyanin]

0.1 ml

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Allophycocyanin

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-98602AF350
Alexa Fluor 405	NBP2-98602AF405
Alexa Fluor 488	NBP2-98602AF488
Alexa Fluor 532	NBP2-98602AF532
Alexa Fluor 594	NBP2-98602AF594
Alexa Fluor 647	NBP2-98602AF647
Alexa Fluor 700	NBP2-98602AF700
Alexa Fluor 750	NBP2-98602AF750
Biotin	NBP2-98602B
CoraFluor 1	NBP2-98602CL1
DyLight 350	NBP2-98602UV
DyLight 405	NBP2-98602V
DyLight 488	NBP2-98602G
DyLight 550	NBP2-98602R
DyLight 594	NBP2-98602DL594
DyLight 650	NBP2-98602C
DyLight 680	NBP2-98602FR
DyLight 755	NBP2-98602IR
FITC	NBP2-98602F
HRP	NBP2-98602H
Janelia Fluor 525	NBP2-98602JF525
Janelia Fluor 549	NBP2-98602JF549
Janelia Fluor 646	NBP2-98602JF646
Janelia Fluor 669	NBP2-98602JF669
PE	NBP2-98602PE
PerCP	NBP2-98602PCP
mFluor Violet 450 SE	NBP2-98602MFV450
mFluor Violet 500 SE	NBP2-98602MFV500
mFluor Violet 610 SE	NBP2-98602MFV610

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