| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41288-0.025mg | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 488 | DDX0910A488-100 | |||
| Alexa Fluor 546 | DDX0910A546-100 | |||
| Alexa Fluor 647 | DDX0910A647-100 | |||
| HRP | DDX0910-HRPO-100 | |||
HAF018). A specific band was detected for a-Synuclein at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human brain (cerebellum) tissue, mouse brain (cerebellum) tissue, and rat brain (cerebellum) tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat a-Synuclein Monoclonal Antibody (Catalog # MAB13381) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a-Synuclein at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB13381-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB13382-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-47338-25ul | |||
![Western Blot: pan-Synuclein Antibody [NBP3-13218] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with pan-Synuclein antibody (NBP3-13218) diluted at 1:1000.](http://images.novusbio.com/fullsize/pan-Synuclein-Antibody-Western-Blot-NBP3-13218-img0001.jpg "Western Blot: pan-Synuclein Antibody [NBP3-13218] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with pan-Synuclein antibody (NBP3-13218) diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: pan-Synuclein Antibody [NBP3-13218] - pan-Synuclein antibody detects pan-Synuclein protein by immunofluorescent analysis. Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: pan-Synuclein protein stained by pan-Synuclein antibody (NBP3-13218) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/pan-Synuclein-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13218-img0005.jpg)
![Immunohistochemistry-Frozen: pan-Synuclein Antibody [NBP3-13218] - pan-Synuclein antibody detects pan-Synuclein protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse hippocampus. Green: pan-Synuclein protein stained by pan-Synuclein antibody (NBP3-13218) diluted at 1:250. Red: NeuN, stained by NeuN antibody [2Q158] diluted at 1:500.](http://images.novusbio.com/fullsize/pan-Synuclein-Antibody-Immunohistochemistry-Frozen-NBP3-13218-img0006.jpg)
![Immunohistochemistry-Paraffin: pan-Synuclein Antibody [NBP3-13218] - pan-Synuclein antibody detects pan-Synuclein protein at cell membrane in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. pan-Synuclein antibody (NBP3-13218) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/pan-Synuclein-Antibody-Immunohistochemistry-Paraffin-NBP3-13218-img0002.jpg)
![Immunohistochemistry-Paraffin: pan-Synuclein Antibody [NBP3-13218] - pan-Synuclein antibody detects pan-Synuclein protein at cell membrane in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. pan-Synuclein antibody (NBP3-13218) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/pan-Synuclein-Antibody-Immunohistochemistry-Paraffin-NBP3-13218-img0003.jpg)
![alpha Synuclein antibody detects alpha Synuclein protein expression by immunohistochemical analysis.<br>Sample: Frozen-sectioned adult mouse cerebellum. <br>Green: alpha Synuclein protein stained by alpha Synuclein antibody (NBP3-13218) diluted at 1:250.<br>Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.<br>Blue: Fluoroshield with DAPI .<br>](http://images.novusbio.com/fullsize/nbp3-13218_rabbit-pan-synuclein-pab-immunohistochemistry-frozen-26112025183681.jpg)
![Western Blot: alpha-Synuclein Antibody [NBP2-41288] - Analysis of 1 ng of recombinant human SNCA with SNCA antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-Western-Blot-NBP2-41288-img0004.jpg "Western Blot: alpha-Synuclein Antibody [NBP2-41288] - Analysis of 1 ng of recombinant human SNCA with SNCA antibody at 1 ug/ml.")
![Western Blot: alpha-Synuclein Antibody [NBP2-41288] - Western blot analysis of SNCA in mouse cerebellum tissue lysate with SNCA antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-Western-Blot-NBP2-41288-img0001.jpg)
![Immunohistochemistry: alpha-Synuclein Antibody - BSA Free [NBP2-41288] - Immunohistochemistry of alpha-Synuclein in rat brain tissue with alpha-Synuclein antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/nbp2-41288_rabbit-polyclonal-alpha-synuclein-antibody-2711202419241375.jpg)
![Immunocytochemistry/ Immunofluorescence: alpha-Synuclein Antibody - BSA Free [NBP2-41288] - Immunofluorescence of alpha-Synuclein in rat brain tissue with alpha-Synuclein antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/nbp2-41288_rabbit-polyclonal-alpha-synuclein-antibody-27112024201327.jpg)
![Immunohistochemistry: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - Immunohistochemistry analysis of rat brain injected with Type 1 mouse alpha synuclein PFFs (NBP2-61596). Species: Female Sprague-Dawley Rat. Rat was injected with 16g Type 1 mouse alpha synuclein PFFs (NBP2-61596) in each of 2 injection sites: AP+1.6, ML+2.4, DV-4.2 from skull; and AP-1.4, ML+0.2, DV-2.8 from skull. 30 days post-injection. Fixation: Saline perfusion followed by 4% PFA fixation for 48 hrs. Primary antibody: rabbit monoclonal anti-pSer129 alpha synuclein. Secondary Antibody: Biotin-SP Donkey Anti-Rabbit IgG (H+L) at 1:500 for 2 hours in cold room with shaking. ABC signal amplification, DAB staining. Magnification: 20X. Alpha synuclein pathology is seen in the periform/insular cortex and the cingulate cortex on both the same (ipsi) and opposite (contra) sides as the injection sites.](http://images.novusbio.com/fullsize/Recombinant-Mouse-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Immunohistochemistry-NBP2-61596-img0003.jpg "Immunohistochemistry: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - Immunohistochemistry analysis of rat brain injected with Type 1 mouse alpha synuclein PFFs (NBP2-61596). Species: Female Sprague-Dawley Rat. Rat was injected with 16g Type 1 mouse alpha synuclein PFFs (NBP2-61596) in each of 2 injection sites: AP+1.6, ML+2.4, DV-4.2 from skull; and AP-1.4, ML+0.2, DV-2.8 from skull. 30 days post-injection. Fixation: Saline perfusion followed by 4% PFA fixation for 48 hrs. Primary antibody: rabbit monoclonal anti-pSer129 alpha synuclein. Secondary Antibody: Biotin-SP Donkey Anti-Rabbit IgG (H+L) at 1:500 for 2 hours in cold room with shaking. ABC signal amplification, DAB staining. Magnification: 20X. Alpha synuclein pathology is seen in the periform/insular cortex and the cingulate cortex on both the same (ipsi) and opposite (contra) sides as the injection sites.")
![In vitro assay: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - Active alpha synuclein aggregate seeds the formation of new alpha Synuclein aggregates from the pool of active monomers. Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha Synuclein aggregates. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha Synuclein protein aggregation) over time when 10 nM of active alpha Synuclein aggregate is combined with 100 uM of active alpha Synuclein monomer, as compared to active alpha Synuclein aggregate and active alpha Synuclein monomer alone. Thioflavin T excitation maximum = 450 nm; emission maximum = 485 nm.](http://images.novusbio.com/fullsize/Recombinant-Mouse-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-In-vitro-assay-NBP2-61596-img0002.jpg)
![Electron Microscopy: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - TEM of Type 1 mouse alpha synuclein Pre-formed Fibrils (NBP2-61596). Fibrils were sonicated and image was taken at 100kx magnification.](http://images.novusbio.com/fullsize/Recombinant-Mouse-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Electron-Microscopy-NBP2-61596-img0005.jpg)
![Electron Microscopy: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - TEM of Type 1 mouse alpha synuclein Pre-formed Fibrils (NBP2-61596). Image was taken at 100kx magnification.](http://images.novusbio.com/fullsize/Recombinant-Mouse-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Electron-Microscopy-NBP2-61596-img0004.jpg)
![Immunohistochemistry: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - TH fluorescence in mouse brain. Image from a verified customer review.](http://images.novusbio.com/fullsize/nbp2-61596_recombinant-mouse-alpha-synuclein-active-pre-formed-fibrils-type-1-protein-immunohistochemistry-205202512497..jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein Antibody (AS11) [DDX0910P-100] - IF staining of human neuroblastoma (SH-SY5Y) expressing endogenous alpha-synuclein](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-AS11-Immunocytochemistry-Immunofluorescence-DDX0910P-100-img0004.jpg "<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: alpha-Synuclein Antibody (AS11) [DDX0910P-100] - IF staining of human neuroblastoma (SH-SY5Y) expressing endogenous alpha-synuclein")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: alpha-Synuclein Antibody (AS11) [DDX0910P-100] - IHC staining of pathological human brains with AS11](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-AS11-Immunohistochemistry-DDX0910P-100-img0005.jpg)
CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to synaptic varicosities. View our protocol for ![SDS-Page: Recombinant Human alpha-Synuclein Protein [NBC1-18331] - alpha-Synuclein E46K, 14.4 (140 aa) , confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE](http://images.novusbio.com/fullsize/alpha-Synuclein-Protein-SDS-Page-NBC1-18331-img0002.jpg "SDS-Page: Recombinant Human alpha-Synuclein Protein [NBC1-18331] - alpha-Synuclein E46K, 14.4 (140 aa) , confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE")
![Immunocytochemistry/Immunofluorescence: Synuclein alpha/beta Antibody (3B6) [NBP1-22957] - Analysis of LNCaP cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti human Synuclein alpha/beta Antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-3B6-Immunocytochemistry-Immunofluorescence-NBP1-22957-img0003.jpg "Immunocytochemistry/Immunofluorescence: Synuclein alpha/beta Antibody (3B6) [NBP1-22957] - Analysis of LNCaP cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti human Synuclein alpha/beta Antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).")
![Western Blot: Synuclein alpha/beta Antibody (3B6) [NBP1-22957] - The recombinant human synuclein family (alpha-,beta- and gamma-) (each 20ng), Mouse brain and Rat brain (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human alpha,beta-synuclein (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-3B6-Western-Blot-NBP1-22957-img0002.jpg)
![Flow Cytometry: Synuclein alpha/beta Antibody (3B6) [NBP1-22957] - Analysis of alpha, beta-Synuclein in C6 cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-3B6-Flow-Cytometry-NBP1-22957-img0006.jpg)
![Flow Cytometry: Synuclein alpha/beta Antibody (3B6) [NBP1-22957] - Analysis of LNCaP cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-3B6-Flow-Cytometry-NBP1-22957-img0004.jpg)
![Flow Cytometry: Synuclein alpha/beta Antibody (3B6) [NBP1-22957] - Analysis of alpha, beta-Synuclein in U87MG cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-3B6-Flow-Cytometry-NBP1-22957-img0005.jpg)
![SDS-Page: Recombinant Human alpha-Synuclein Active, Monomer, (Type 1) Protein [NBP2-54788] - 14kDa Human Recombinant Alpha Synuclein Protein Monomer. Lane 1: Molecular Weight Ladder (MW). Lane 2: Alpha Synuclein Protein Monomer (2 ug).](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Monomer-Type-1-Protein-SDS-Page-NBP2-54788-img0006.jpg "SDS-Page: Recombinant Human alpha-Synuclein Active, Monomer, (Type 1) Protein [NBP2-54788] - 14kDa Human Recombinant Alpha Synuclein Protein Monomer. Lane 1: Molecular Weight Ladder (MW). Lane 2: Alpha Synuclein Protein Monomer (2 ug).")
![In vitro assay: Recombinant Human alpha-Synuclein Active, Monomer, (Type 1) Protein [NBP2-54788] - Thioflavin T emission curves show increased fluorescence (correlated to alpha Synuclein protein aggregation) over time when 10 nM of active alpha Synuclein aggregate (NBP2-54789) is combined with 100 uM of active alpha Synuclein monomer (NBP2-54788), as compared to when 100 uM of control alpha Synuclein monomer (NBP2-54786) is combined with 10 nM of active alpha Synuclein aggregate (NBP2-54789). Thioflavin T ex = 450 nm, em = 485 nm.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Monomer-Type-1-Protein-In-vitro-assay-NBP2-54788-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein Antibody (970106) [MAB13382] - Analysis using immersion fixed SK-Mel-28 human cytoplasm using SNCA antibody.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-970106-Immunocytochemistry-Immunofluorescence-MAB13382-img0001.jpg "Immunocytochemistry/Immunofluorescence: alpha-Synuclein Antibody (970106) [MAB13382] - Analysis using immersion fixed SK-Mel-28 human cytoplasm using SNCA antibody.")
![Western Blot: pan-Synuclein Antibody [NBP2-47338] - Analysis in human cell lines SK-MEL-30 and MCF-7 using anti-SNCA antibody. Corresponding SNCA RNA-seq data are presented for the same cell lines. Loading control: anti-PFN1.](http://images.novusbio.com/fullsize/pan-Synuclein-Antibody-Western-Blot-NBP2-47338-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: pan-Synuclein Antibody [NBP2-47338] - Analysis in human cell lines SK-MEL-30 and MCF-7 using anti-SNCA antibody. Corresponding SNCA RNA-seq data are presented for the same cell lines. Loading control: anti-PFN1.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: pan-Synuclein Antibody [NBP2-47338] - Staining in human cerebral cortex and liver tissues using anti-SNCA antibody. Corresponding SNCA RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/pan-Synuclein-Antibody-Immunohistochemistry-Paraffin-NBP2-47338-img0007.jpg)
![Western Blot: pan-Synuclein Antibody [NBP2-47338] - Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17. Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/pan-Synuclein-Antibody-Western-Blot-NBP2-47338-img0002.jpg)
![Immunohistochemistry: pan-Synuclein Antibody [NBP2-47338] - Staining of human cerebral cortex shows strongly immunoreactivity neuropil and weak cytoplasmic positivity in neuronal cells, glial cells.](http://images.novusbio.com/fullsize/pan-Synuclein-Antibody-Immunohistochemistry-NBP2-47338-img0001.jpg)
![Immunohistochemistry-Paraffin: pan-Synuclein Antibody [NBP2-47338] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/pan-Synuclein-Antibody-Immunohistochemistry-Paraffin-NBP2-47338-img0005.jpg)
![Immunohistochemistry-Paraffin: pan-Synuclein Antibody [NBP2-47338] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/pan-Synuclein-Antibody-Immunohistochemistry-Paraffin-NBP2-47338-img0006.jpg)
![Western Blot: alpha-Synuclein [p Ser 129] Antibody (J18) [NBP3-18266] - Western Blot analysis of Human Alpha Synuclein showing detection of alpha-Synuclein protein using Rabbit Anti-alpha-Synuclein Monoclonal Antibody, Clone J18 (NBP3-18266). Lane 1: MW ladder. Lane 2: 0.5 ug human alpha synuclein monomer (SPR-321). Lane 3: 2 ug human alpha synuclein monomer (SPR-321). Lane 4: 0.5 ug human alpha synuclein PFFs (SPR-322). Lane 5: 2 ug human alpha synuclein PFFs (SPR-322). Lane 6: 15 ug human Parkinson's Disease brain lysate.. Block: 5% BSA in TBST. Primary Antibody: Rabbit Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18266) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. NBP3-18266 does not detect unphosphorylated alpha synuclein.](http://images.novusbio.com/fullsize/alpha-Synuclein-p-Ser-129-Antibody-J18-Western-Blot-NBP3-18266-img0004.jpg "Western Blot: alpha-Synuclein [p Ser 129] Antibody (J18) [NBP3-18266] - Western Blot analysis of Human Alpha Synuclein showing detection of alpha-Synuclein protein using Rabbit Anti-alpha-Synuclein Monoclonal Antibody, Clone J18 (NBP3-18266). Lane 1: MW ladder. Lane 2: 0.5 ug human alpha synuclein monomer (SPR-321). Lane 3: 2 ug human alpha synuclein monomer (SPR-321). Lane 4: 0.5 ug human alpha synuclein PFFs (SPR-322). Lane 5: 2 ug human alpha synuclein PFFs (SPR-322). Lane 6: 15 ug human Parkinson's Disease brain lysate.. Block: 5% BSA in TBST. Primary Antibody: Rabbit Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18266) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. NBP3-18266 does not detect unphosphorylated alpha synuclein.")
![Immunohistochemistry: alpha-Synuclein [p Ser 129] Antibody (J18) [NBP3-18266] - C57/BL6 mice were injected with sonicated recombinant mouse alpha synuclein monomers or fibrils at 8 weeks of age. Mice were unilaterally injected in the dorsal striatum (bregma AP + 0.2 mm, L +/1 2.0 mm, V - 3.0 mm) and sacrificed 30 days post-injection. (A) 1.25 uL mouse alpha synuclein monomers. (B) 2.5 uL mouse alpha synuclein monomers. (C) 2.5 ug alpha synuclein PFFs. (C) 5 ug alpha synuclein PFFs Inset: PBS (negative control). Primary antibody: Anti-alpha-Synuclein (NBP3-18266) at 1:10 000. Secondary antibody: anti-rabbit HRP. Mice injected with PFF displayed alpha synuclein staining in the striatum and cortex and contralateral to the injection site.](http://images.novusbio.com/fullsize/alpha-Synuclein-p-Ser-129-Antibody-J18-Immunohistochemistry-NBP3-18266-img0002.jpg)
![Western Blot: alpha-Synuclein [p Ser 129] Antibody (J18) [NBP3-18266] - Western Blot analysis of Mouse Brain showing detection of alpha-Synuclein protein using Rabbit Anti-alpha-Synuclein Monoclonal Antibody, Clone J18 (NBP3-18266). Lane 1: MW ladder. Lane 2: Mouse brain. Load: 15 ug. Block: 5% BSA in TBST. Primary Antibody: Rabbit Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18266) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Multiple bands due to phosphorylation.](http://images.novusbio.com/fullsize/alpha-Synuclein-p-Ser-129-Antibody-J18-Western-Blot-NBP3-18266-img0005.jpg)
![Immunohistochemistry: alpha-Synuclein [p Ser 129] Antibody (J18) [NBP3-18266] - Immunohistochemistry analysis using Mouse Anti-alpha-Synuclein Monoclonal Antibody, Clone J18 (NBP3-18266). C57/BL6 mice were injected with 5 ug sonicated mouse recombinant alpha synuclein PFFs at 8 weeks of age. Mice were unilaterally injected in the dorsal striatum (bregma AP + 0.2 mm, L +/1 2.0 mm, V - 3.0 mm) and sacrificed 30 days post-injection. (A) contralateral cortex. (B) ipsilateral cortex. (C) contralateral striatum. (D) ipsilateral striatum. Primary Antibody: Mouse Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18266) at 1:10000. Secondary Antibody: anti-rabbit HRP. Courtesy of: Porsolt.](http://images.novusbio.com/fullsize/alpha-Synuclein-p-Ser-129-Antibody-J18-Immunohistochemistry-NBP3-18266-img0001.jpg)
![Dot Blot: alpha-Synuclein [p Ser 129] Antibody (J18) [NBP3-18266] - Dot Blot analysis using Rabbit Anti-alpha-Synuclein Monoclonal Antibody, Clone J18 (NBP3-18266). Tissue: alpha synuclein peptide. Primary Antibody: Rabbit Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18266) at 1:500 for 2 hours at RT with shaking . Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking . Phospho peptide sequence: AYEMP-pS-EEGYQ. Non-phospho peptide sequence: AYEMPSEEGYQ. This sequence is the same for human, mouse, and rat.](http://images.novusbio.com/fullsize/alpha-Synuclein-p-Ser-129-Antibody-J18-Dot-Blot-NBP3-18266-img0003.jpg)
![Western Blot: alpha-Synuclein Antibody (4F1) [NBP3-18259] - Western Blot analysis of Human, Mouse, Rat Brain showing detection of 14 kDa alpha-Synuclein protein using Mouse Anti-alpha-Synuclein Monoclonal Antibody, Clone 4F1 (NBP3-18259). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain cell lysate. Lane 3: Rat brain cell lysate. Lane 4: Human brain cell lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18259) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 14 kDa. Other Band(s): ~40 kDa (trimer).](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-4F1-Western-Blot-NBP3-18259-img0002.jpg "Western Blot: alpha-Synuclein Antibody (4F1) [NBP3-18259] - Western Blot analysis of Human, Mouse, Rat Brain showing detection of 14 kDa alpha-Synuclein protein using Mouse Anti-alpha-Synuclein Monoclonal Antibody, Clone 4F1 (NBP3-18259). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain cell lysate. Lane 3: Rat brain cell lysate. Lane 4: Human brain cell lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18259) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 14 kDa. Other Band(s): ~40 kDa (trimer).")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: alpha-Synuclein Antibody (4F1) [NBP3-18259] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-alpha-Synuclein Monoclonal Antibody, Clone 4F1 (NBP3-18259). Tissue: Primary hippocampal neurons treated with active alpha-Synuclein Protein Aggregate (SPR-322) at 4 ug/ml to induce fibrils. Species: Rat. Fixation: 4% paraformaldehyde. Primary Antibody: Mouse Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18259) at 1:200 for 24 hours at 4C. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:700 for 1 hour at RT. Counterstain: Guinea Pig Anti-NeuN (red) neuronal marker (Donkey Anti-Guinea Pig Alexa Fluor 647 1:700); DAPI (blue) nuclear stain at 1:6000, 1:3000 for 60 min at RT, 5 min at RT. Magnification: 20X. (A) DAPI (blue) nuclear stain. (B) NeuN neuronal marker (red). (C) alpha-Synuclein Antibody. (D) Composite.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-4F1-Immunocytochemistry-Immunofluorescence-NBP3-18259-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein Antibody (4F1) [NBP3-18259] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-alpha-Synuclein Monoclonal Antibody, Clone 4F1 (NBP3-18259). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18259) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm: medium; Nucleus: strong. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) alpha-Synuclein Antibody. (D) Composite.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-4F1-Immunocytochemistry-Immunofluorescence-NBP3-18259-img0001.jpg)
