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![Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody - BSA Free [NBP1-03074] - Staining of human placenta. Tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.](http://images.novusbio.com/fullsize/SIRP-alpha-CD172a-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP1-03074-img0004.jpg "Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody - BSA Free [NBP1-03074] - Staining of human placenta. Tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.")
![Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody - BSA Free [NBP1-03074] - Staining of human testis. Tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.](http://images.novusbio.com/fullsize/SIRP-alpha-CD172a-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP1-03074-img0003.jpg)
![Western Blot: SIRP alpha/CD172a Antibody (JE36-20) [NBP3-32950] - Western blot analysis of SIRP alpha/CD172a on different lysates with Rabbit anti-SIRP alpha/CD172a antibody (NBP3-32950) at 1/500 dilution. Lane 1: THP-1 cell lysate (10 ug/Lane) Lane 2: HL-60 cell lysate (10 ug/Lane) Lane 3: Mouse brain tissue lysate (20 ug/Lane) Predicted band size: 55 kDa Observed band size: 75-100 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32950) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32950_rabbit-sirp-alpha-cd172a-mab-je36-20-288202412442941.png "Western Blot: SIRP alpha/CD172a Antibody (JE36-20) [NBP3-32950] - Western blot analysis of SIRP alpha/CD172a on different lysates with Rabbit anti-SIRP alpha/CD172a antibody (NBP3-32950) at 1/500 dilution. Lane 1: THP-1 cell lysate (10 ug/Lane) Lane 2: HL-60 cell lysate (10 ug/Lane) Lane 3: Mouse brain tissue lysate (20 ug/Lane) Predicted band size: 55 kDa Observed band size: 75-100 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32950) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: SIRP alpha/CD172a Antibody (JE36-20) [NBP3-32950] - Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-SIRP alpha/CD172a antibody (NBP3-32950) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32950) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32950_rabbit-sirp-alpha-cd172a-mab-je36-20-298202413585563.jpg)
![Flow Cytometry: SIRP alpha/CD172a Antibody (JE36-20) [NBP3-32950] - Flow cytometric analysis of THP-1 cells labeling SIRP alpha/CD172a. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32950, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32950_rabbit-sirp-alpha-cd172a-mab-je36-20-2920241637213.png)
![Western Blot: SIRP alpha/CD172a Antibody [NBP1-77045] - Analysis of SIRP alpha in THP-1 whole cell lysate with anti-SIRP alpha using this antibody at 1:2000 dilution.](http://images.novusbio.com/fullsize/SIRP-alpha-CD172a-Antibody-Western-Blot-NBP1-77045-img0003.jpg "Western Blot: SIRP alpha/CD172a Antibody [NBP1-77045] - Analysis of SIRP alpha in THP-1 whole cell lysate with anti-SIRP alpha using this antibody at 1:2000 dilution.")
![Immunocytochemistry/ Immunofluorescence: SIRP alpha/CD172a Antibody - BSA Free [NBP1-77045] - Immunocytochemistry of SIRP alpha in THP-1 cells with SIRP alpha antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77045_rabbit-polyclonal-sirp-alpha-cd172a-antibody-2711202420441151.jpg)
![Flow Cytometry: SIRP alpha/CD172a Antibody (DH59B) [NBP2-61014] - Obtained with bovine leukocytes](http://images.novusbio.com/fullsize/SIRP-alpha-CD172a-Antibody-DH59B-Flow-Cytometry-NBP2-61014-img0001.jpg "Flow Cytometry: SIRP alpha/CD172a Antibody (DH59B) [NBP2-61014] - Obtained with bovine leukocytes")
![Western Blot: SIRP alpha/CD172a Antibody (S08-1G4) [NBP3-14877] - Western blot detection of SIRP alpha/CD172a in A549, MCF-7, 293T cell lysates using SIRP alpha/CD172a Rabbit mAb (1:1000 diluted). Predicted band size: 55kDa. Observed band size: 85kDa.](http://images.novusbio.com/fullsize/SIRP-alpha-CD172a-Antibody-S08-1G4-Western-Blot-NBP3-14877-img0001.jpg "Western Blot: SIRP alpha/CD172a Antibody (S08-1G4) [NBP3-14877] - Western blot detection of SIRP alpha/CD172a in A549, MCF-7, 293T cell lysates using SIRP alpha/CD172a Rabbit mAb (1:1000 diluted). Predicted band size: 55kDa. Observed band size: 85kDa.")
![Immunocytochemistry/Immunofluorescence: SIRP alpha/CD172a Antibody (S08-1G4) [NBP3-14877] - Immunocytochemistry of SIRP alpha/CD172a (green) in hela using NBP3-14877 at dilution 1/50, and DAPI (blue)](http://images.novusbio.com/fullsize/nbp3-14877_rabbit-sirp-alpha-cd172a-mab-s08-1g4-113202515145470.jpg)
![Western Blot: SIRP alpha/CD172a Antibody (S04-7G6) [NBP3-19772] - Western blot detection of SIRP alpha/CD172a in Rat Brain lysates using NBP3-19772 (1:1000 diluted). Predicted band size: 55kDa. Observed band size: 85kDa.](http://images.novusbio.com/fullsize/SIRP-alpha-CD172a-Antibody-S04-7G6-Western-Blot-NBP3-19772-img0001.jpg "Western Blot: SIRP alpha/CD172a Antibody (S04-7G6) [NBP3-19772] - Western blot detection of SIRP alpha/CD172a in Rat Brain lysates using NBP3-19772 (1:1000 diluted). Predicted band size: 55kDa. Observed band size: 85kDa.")
![Western Blot: SIRP alpha Overexpression Lysate (Adult Normal) [NBP2-11199] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SIRP alpha.](http://images.novusbio.com/fullsize/SIRP-alpha-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11199-img0001.jpg "Western Blot: SIRP alpha Overexpression Lysate (Adult Normal) [NBP2-11199] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SIRP alpha.")
![Western Blot: SIRP alpha Overexpression Lysate (Adult Normal) [NBP2-05765] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SIRP alpha.](http://images.novusbio.com/fullsize/SIRP-alpha-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-05765-img0001.jpg "Western Blot: SIRP alpha Overexpression Lysate (Adult Normal) [NBP2-05765] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SIRP alpha.")
![Western Blot: SIRP alpha Overexpression Lysate (Adult Normal) [NBL1-15971] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SIRP alpha.](http://images.novusbio.com/fullsize/SIRP-alpha-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-15971-img0002.jpg "Western Blot: SIRP alpha Overexpression Lysate (Adult Normal) [NBL1-15971] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SIRP alpha.")
![Flow Cytometry: SIRP alpha/CD172a Antibody (001) [NBP2-90751] - Flow cytometric analysis of Mouse SIRPA(CD172a) expression on BABL/c bone marrow cells. Cells were stained with purified anti-Mouse SIRPA(CD172a), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/SIRP-alpha-CD172a-Antibody-001-Flow-Cytometry-NBP2-90751-img0001.jpg "Flow Cytometry: SIRP alpha/CD172a Antibody (001) [NBP2-90751] - Flow cytometric analysis of Mouse SIRPA(CD172a) expression on BABL/c bone marrow cells. Cells were stained with purified anti-Mouse SIRPA(CD172a), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")