HAF008). A specific band was detected for Serpin E1/PAI-1 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HUVEC human umbilical vein endothelial cells and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Serpin E1/PAI-1 Monoclonal Antibody (Catalog # MAB17861) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Serpin E1/PAI-1 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB17861-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB3828-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59495-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26327-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21747-50ul | |||
![ELISA: Rat Serpin E1/PAI-1 ELISA Kit (Colorimetric) [NBP3-18763] - Samples were spiked with high concentrations of Rat Serpin E1/PAI-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-18763_rat-serpin-e1-pai-1-elisa-kit-colorimetric-13220241623233.jpg "ELISA: Rat Serpin E1/PAI-1 ELISA Kit (Colorimetric) [NBP3-18763] - Samples were spiked with high concentrations of Rat Serpin E1/PAI-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Serpin E1/PAI-1 ELISA Kit (Colorimetric) [NBP3-18763] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-18763_rat-serpin-e1-pai-1-elisa-kit-colorimetric-432024941562.jpg)
![Western Blot: Serpin E1/PAI-1 Antibody (1C5) [NBP2-59495] - Lane 1: 293T cell lysates, Lane 2: PAI1 transfected 293T cell lysates](http://images.novusbio.com/fullsize/Serpin-E1-PAI-1-Antibody-1C5-Western-Blot-NBP2-59495-img0001.jpg "Western Blot: Serpin E1/PAI-1 Antibody (1C5) [NBP2-59495] - Lane 1: 293T cell lysates, Lane 2: PAI1 transfected 293T cell lysates")
![Immunocytochemistry/Immunofluorescence: Serpin E1/PAI-1 Antibody (1C5) [NBP2-59495] - Analysis of PAI1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PAI1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green)](http://images.novusbio.com/fullsize/Serpin-E1-PAI-1-Antibody-1C5-Immunocytochemistry-Immunofluorescence-NBP2-59495-img0002.jpg)
![Immunohistochemistry: Serpin E1/PAI-1 Antibody (10C3) [NBP3-26327] - Image of Serpin E1/PAI-1 Antibody (10C3) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26327_rabbit-serpin-e1-pai-1-mab-10c3-2722024827491.jpg "Immunohistochemistry: Serpin E1/PAI-1 Antibody (10C3) [NBP3-26327] - Image of Serpin E1/PAI-1 Antibody (10C3) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: Serpin E1/PAI-1 Antibody (10C3) [NBP3-26327] - Positive Western Blot detected in: U-251 whole cell lysate, U-87 whole cell lysate. All lanes: PAI1 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 46, 44 kDa Observed band size: 45 kDa](http://images.novusbio.com/fullsize/nbp3-26327_rabbit-serpin-e1-pai-1-mab-10c3-28220241140947.jpg)
![Western Blot: Serpin E1/PAI-1 Antibody (SR1779) [NBP3-21747] - Western blot analysis using NBP3-21747 in HepG2 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21747_rabbit-serpin-e1-pai-1-mab-sr1779-57202315123419.jpg "Western Blot: Serpin E1/PAI-1 Antibody (SR1779) [NBP3-21747] - Western blot analysis using NBP3-21747 in HepG2 cell lysate.")
![ELISA: Mouse Serpin E1/PAI-1 ELISA Kit (Colorimetric) [NBP3-18780] - Samples were spiked with high concentrations of Mouse Serpin E1/PAI-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-18780_mouse-serpin-e1-pai-1-elisa-kit-colorimetric-132202416133688.jpg "ELISA: Mouse Serpin E1/PAI-1 ELISA Kit (Colorimetric) [NBP3-18780] - Samples were spiked with high concentrations of Mouse Serpin E1/PAI-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Serpin E1/PAI-1 ELISA Kit (Colorimetric) [NBP3-18780] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-18780_mouse-serpin-e1-pai-1-elisa-kit-colorimetric-432024955042.jpg)
![Western Blot: PAI1/Serpine1 Overexpression Lysate (Native) [NBP2-09008] - PAI1/Serpine1 Overexpression Lysate (Native) Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Serpine1.](http://images.novusbio.com/fullsize/PAI1-Serpine1-Overexpression-Lysate-Native-Western-Blot-NBP2-09008-img0002.jpg "Western Blot: PAI1/Serpine1 Overexpression Lysate (Native) [NBP2-09008] - PAI1/Serpine1 Overexpression Lysate (Native) Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Serpine1.")
