1290-IL) were stained with Mouse Anti-Human/Mouse ROR gammat/RORC2/NR1F3 PerCP-conjugated Monoclonal Antibody (Catalog # IC6006C) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog #
FAB100A). Quadrant markers were set based on control antibody staining (Catalog #
IC0041C). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog #
FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog #
FC005). View our protocol for
Staining Intracellular Molecules." class="big_lightbox">

1290-IL) were stained with Mouse Anti-Human/Mouse ROR gammat/RORC2/NR1F3 PerCP-conjugated Monoclonal Antibody (Catalog # IC6006C) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog #
FAB100A). Quadrant markers were set based on control antibody staining (Catalog #
IC0041C). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog #
FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog #
FC005). View our protocol for
Staining Intracellular Molecules." alt="Human peripheral blood mononuclear cells (PBMCs) treated with PMA, Calcium Ionomycin, LPS, and Recombinant Human IL-23 (Catalog #
1290-IL) were stained with Mouse Anti-Human/Mouse ROR gammat/RORC2/NR1F3 PerCP-conjugated Monoclonal Antibody (Catalog # IC6006C) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog #
FAB100A). Quadrant markers were set based on control antibody staining (Catalog #
IC0041C). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog #
FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog #
FC005). View our protocol for
Staining Intracellular Molecules." class="big_thumb" />
IC0041C, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog #
FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog #
FC005). View our protocol for
Staining Intracellular Molecules." class="big_lightbox">

IC0041C, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog #
FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog #
FC005). View our protocol for
Staining Intracellular Molecules." class="big_thumb" />