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![Western Blot: R3HDM1 Antibody [NBP2-41362] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-R3HDM1 antibody NBP2-41362 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/R3HDM1-Antibody-Western-Blot-NBP2-41362-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: R3HDM1 Antibody [NBP2-41362] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-R3HDM1 antibody NBP2-41362 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: R3HDM1 Antibody [NBP2-41362] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-R3HDM1 antibody NBP2-41362 used for IP at 6 ug per reaction. R3HDM1 was also immunoprecipitated by rabbit anti-R3HDM1 antibody BL18127. For blotting immunoprecipitated R3HDM1, it was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/R3HDM1-Antibody-Immunoprecipitation-NBP2-41362-img0004.jpg)