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![Western Blot: PRB4 Antibody [NBP3-05584] - Western blot analysis of extracts of various cell lines, using PRB4 antibody (NBP3-05584) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/PRB4-Antibody-Western-Blot-NBP3-05584-img0001.jpg "Western Blot: PRB4 Antibody [NBP3-05584] - Western blot analysis of extracts of various cell lines, using PRB4 antibody (NBP3-05584) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")