| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24771SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB3647-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62557-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-57913-25ul | |||
![Western Blot: Notch-1 Antibody [NBP2-24771] - Analysis of Notch1 in HEK293 cells transfected with full length Notch 1 A) untreated and B) treated with 5 mM EDTA for 15 minutes, using this antibody at 3 ug/mL. The EDTA treatment increases the detected level of Notch1-cv.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Western-Blot-NBP2-24771-img0002.jpg "Western Blot: Notch-1 Antibody [NBP2-24771] - Analysis of Notch1 in HEK293 cells transfected with full length Notch 1 A) untreated and B) treated with 5 mM EDTA for 15 minutes, using this antibody at 3 ug/mL. The EDTA treatment increases the detected level of Notch1-cv.")
![N/A Notch-1 [Biotin]](http://images.novusbio.com/fullsize2/DATA_Notch1_DY5317_ELISA_2100.jpg "N/A Notch-1 [Biotin]")
![Flow Cytometry: Notch-1 Antibody (E6) - Chimeric [NBP2-62557] - Flow-cytometry using the anti-Notch 1 antibody E6. Mouse thymocytes were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of E6 (NBP2-62557, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Notch-1-Antibody-E6-Chimeric-Flow-Cytometry-NBP2-62557-img0002.jpg "Flow Cytometry: Notch-1 Antibody (E6) - Chimeric [NBP2-62557] - Flow-cytometry using the anti-Notch 1 antibody E6. Mouse thymocytes were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of E6 (NBP2-62557, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.")
![Immunocytochemistry /Immunofluorescence: Notch-1 Antibody (E6) - Chimeric - Azide and BSA Free [NBP2-62557] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with NBP2-62557 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-62557, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-62557_rabbit-monoclonal-notch-1-antibody-e6-chimeric-74202522141235.png)
![Western Blot: Notch-1 Antibody (7L3V4) [NBP3-15658] - Western blot analysis of extracts of various cell lines, using Notch-1 antibody (NBP3-15658) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Notch-1-Antibody-7L3V4-Western-Blot-NBP3-15658-img0001.jpg "Western Blot: Notch-1 Antibody (7L3V4) [NBP3-15658] - Western blot analysis of extracts of various cell lines, using Notch-1 antibody (NBP3-15658) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunoprecipitation: Notch-1 Antibody (7L3V4) [NBP3-15658] - Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug Notch-1 antibody (NBP3-15658). Western blot was performed from the immunoprecipitate using Notch-1 antibody (NBP3-15658) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Notch-1-Antibody-7L3V4-Immunoprecipitation-NBP3-15658-img0002.jpg)
![Western Blot: Notch-1 Antibody (S02-2G9) [NBP3-19583] - Western blot detection of Notch-1 in C6, Hela cell lysates using NBP3-19583 (1:1000 diluted). Predicted band size: 273kDa. Observed band size: 120kDa.](http://images.novusbio.com/fullsize/Notch-1-Antibody-S02-2G9-Western-Blot-NBP3-19583-img0001.jpg "Western Blot: Notch-1 Antibody (S02-2G9) [NBP3-19583] - Western blot detection of Notch-1 in C6, Hela cell lysates using NBP3-19583 (1:1000 diluted). Predicted band size: 273kDa. Observed band size: 120kDa.")
![Immunocytochemistry/Immunofluorescence: Notch-1 Antibody [NBP2-57913] - Staining of human cell line HaCaT shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57913-img0001.jpg "Immunocytochemistry/Immunofluorescence: Notch-1 Antibody [NBP2-57913] - Staining of human cell line HaCaT shows localization to nucleoplasm.")
![Western Blot: Notch1 Overexpression Lysate (Adult Normal) [NBL1-13725] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Notch1.](http://images.novusbio.com/fullsize/Notch1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13725-img0002.jpg "Western Blot: Notch1 Overexpression Lysate (Adult Normal) [NBL1-13725] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Notch1.")