| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59498-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03013-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89930-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73003 | |||
![Western Blot: NM23-H1 Antibody (5F4) [NBP2-59498] - The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NME1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1 : HeLa cell lysateLane 2 : A549 cell lysateLane 3 : Jurkat cell lysateLane 4 : HepG2 cell lysateLane 5 : MCF7 cell lysateLane 6 : PC3 cell lysate](http://images.novusbio.com/fullsize/NM23-H1-Antibody-5F4-Western-Blot-NBP2-59498-img0004.jpg "Western Blot: NM23-H1 Antibody (5F4) [NBP2-59498] - The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NME1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1 : HeLa cell lysateLane 2 : A549 cell lysateLane 3 : Jurkat cell lysateLane 4 : HepG2 cell lysateLane 5 : MCF7 cell lysateLane 6 : PC3 cell lysate")
![Immunocytochemistry/Immunofluorescence: NM23-H1 Antibody (5F4) [NBP2-59498] - Analysis of NME1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human NME1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). Analysis of NME1 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human NME1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/NM23-H1-Antibody-5F4-Immunocytochemistry-Immunofluorescence-NBP2-59498-img0002.jpg)
![Western Blot: NM23-H1 Antibody (5F4) [NBP2-59498] - Lane 1: NME1, Lane 2: NME2 Lane 3: NME3 Lane 4: NME4](http://images.novusbio.com/fullsize/NM23-H1-Antibody-5F4-Western-Blot-NBP2-59498-img0001.jpg)
![Western Blot: NM23-H1 Antibody (13C1-R) [NBP3-33131] - Western blot analysis of NM23-H1 on different lysates with Mouse anti-NM23-H1 antibody (NBP3-33131) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: MCF7 cell lysate Lane 3: Jurkat cell lysate Lane 4: HeLa cell lysate Lane 5: HepG2 cell lysate Lane 6: K-562 cell lysate Lane 7: A549 cell lysate Lane 8: Raji cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 17 kDa Observed band size: 17/20 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33131) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33131_mouse-nm23-h1-mab-13c1-r-288202414191531.jpg "Western Blot: NM23-H1 Antibody (13C1-R) [NBP3-33131] - Western blot analysis of NM23-H1 on different lysates with Mouse anti-NM23-H1 antibody (NBP3-33131) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: MCF7 cell lysate Lane 3: Jurkat cell lysate Lane 4: HeLa cell lysate Lane 5: HepG2 cell lysate Lane 6: K-562 cell lysate Lane 7: A549 cell lysate Lane 8: Raji cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 17 kDa Observed band size: 17/20 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33131) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: NM23-H1 Antibody (13C1-R) [NBP3-33131] - Immunocytochemistry analysis of MCF7 cells labeling NM23-H1 with Mouse anti-NM23-H1 antibody (NBP3-33131) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-NM23-H1 antibody (NBP3-33131) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33131_mouse-nm23-h1-mab-13c1-r-2920241192318.jpg)
![Western Blot: NM23-H1 Antibody (OTI4H2) - Azide and BSA Free [NBP2-73003] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NM23-H1.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-OTI4H2-Azide-and-BSA-Free-Western-Blot-NBP2-73003-img0001.jpg "Western Blot: NM23-H1 Antibody (OTI4H2) - Azide and BSA Free [NBP2-73003] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NM23-H1.")
![Western Blot: NM23-H1 Antibody [NBP3-03013] - Analysis of extracts from normal (control) and NME1 knockout (KO) 293T cells, using NM23-H1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:Basic ECL Kit.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-Knockout-Validated-NBP3-03013-img0001.jpg "<b>Genetic Strategies Validation. </b>Western Blot: NM23-H1 Antibody [NBP3-03013] - Analysis of extracts from normal (control) and NME1 knockout (KO) 293T cells, using NM23-H1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:Basic ECL Kit.")
![Western Blot: nm23-H1 Overexpression Lysate (Native) [NBP2-04154] - nm23-H1 Overexpression Lysate (Native) Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Nm23A.](http://images.novusbio.com/fullsize/nm23-H1-Overexpression-Lysate-Native-Western-Blot-NBP2-04154-img0002.jpg "Western Blot: nm23-H1 Overexpression Lysate (Native) [NBP2-04154] - nm23-H1 Overexpression Lysate (Native) Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Nm23A.")
![Western Blot: nm23-H1 Overexpression Lysate (Native) [NBP2-04713] - nm23-H1 Overexpression Lysate (Native) Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Nm23A.](http://images.novusbio.com/fullsize/nm23-H1-Overexpression-Lysate-Native-Western-Blot-NBP2-04713-img0002.jpg "Western Blot: nm23-H1 Overexpression Lysate (Native) [NBP2-04713] - nm23-H1 Overexpression Lysate (Native) Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Nm23A.")
![Immunohistochemistry-Paraffin: NM23-H1 Antibody (084) [NBP2-89930] - Staining of human NME1 in human hepatoma with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/NM23-H1-Antibody-084-Immunohistochemistry-Paraffin-NBP2-89930-img0002.jpg "Immunohistochemistry-Paraffin: NM23-H1 Antibody (084) [NBP2-89930] - Staining of human NME1 in human hepatoma with rabbit monoclonal antibody (1:200).")
![Immunohistochemistry-Paraffin: NM23-H1 Antibody (084) [NBP2-89930] - Staining of human NME1 in human lung cancer with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/NM23-H1-Antibody-084-Immunohistochemistry-Paraffin-NBP2-89930-img0001.jpg)