| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90043-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21594-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21533-50ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| HRP | NBP2-79383-5Plates | |||
![Western Blot: IkB-alpha [p Tyr42] Antibody [NBP3-23389] - Western blot analysis of A431 cells treated with pervanadate (1 mM) for 30 min. Blots were probed with anti-IkB-alpha (lane 1), anti-IkB-alpha (Tyr-42) (NBP3-23389; lanes 2-5), or anti-IkB-alpha (Tyr-305) (NBP3-23390; lanes 6-9). In some lanes, the antibodies were used in the absence (lane 2 & 6) or presence of IkB-alpha (Tyr-42) (lane 3 & 8) or IkB-alpha (Tyr-305) (lane 4 & 7) blocking peptides, or BSA conjugated to phospho-tyrosine (lane 5 & 9).](http://images.novusbio.com/fullsize/nbp3-23389_rabbit-ikb-alpha-p-tyr42-pab-19920231856943.jpg "Western Blot: IkB-alpha [p Tyr42] Antibody [NBP3-23389] - Western blot analysis of A431 cells treated with pervanadate (1 mM) for 30 min. Blots were probed with anti-IkB-alpha (lane 1), anti-IkB-alpha (Tyr-42) (NBP3-23389; lanes 2-5), or anti-IkB-alpha (Tyr-305) (NBP3-23390; lanes 6-9). In some lanes, the antibodies were used in the absence (lane 2 & 6) or presence of IkB-alpha (Tyr-42) (lane 3 & 8) or IkB-alpha (Tyr-305) (lane 4 & 7) blocking peptides, or BSA conjugated to phospho-tyrosine (lane 5 & 9).")
![Western Blot: IkB-alpha Antibody [NBP3-23384] - Western blot image of human A431. The Blots were probed with anti-IkB-alpha (C-term.) polyclonal antibody at a dilution of 1:500 (lane 1), 1:1000 (lane 2), and 1:2000 (lane 3).](http://images.novusbio.com/fullsize/nbp3-23384_rabbit-ikb-alpha-pab-19920231759443.jpg "Western Blot: IkB-alpha Antibody [NBP3-23384] - Western blot image of human A431. The Blots were probed with anti-IkB-alpha (C-term.) polyclonal antibody at a dilution of 1:500 (lane 1), 1:1000 (lane 2), and 1:2000 (lane 3).")
![Western Blot: IkB-alpha [p Tyr305] Antibody [NBP3-23390] - Western blot analysis of A431 cells treated with pervanadate (1 mM) for 30 min. Blots were probed with anti-IkB-alpha (lane 1), anti-IkB-alpha (Tyr-42) (NBP3-23389; lanes 2-5), or anti-IkB-alpha (Tyr-305) (NBP3-23390; lanes 6-9). In some lanes, the antibodies were used in the absence (lane 2 & 6) or presence of IkB-alpha (Tyr-42) (lane 3 & 8) or IkB-alpha (Tyr-305) (lane 4 & 7) blocking peptides, or BSA conjugated to phospho-tyrosine (lane 5 & 9).](http://images.novusbio.com/fullsize/nbp3-23390_rabbit-ikb-alpha-p-tyr305-pab-18920231735522.jpg "Western Blot: IkB-alpha [p Tyr305] Antibody [NBP3-23390] - Western blot analysis of A431 cells treated with pervanadate (1 mM) for 30 min. Blots were probed with anti-IkB-alpha (lane 1), anti-IkB-alpha (Tyr-42) (NBP3-23389; lanes 2-5), or anti-IkB-alpha (Tyr-305) (NBP3-23390; lanes 6-9). In some lanes, the antibodies were used in the absence (lane 2 & 6) or presence of IkB-alpha (Tyr-42) (lane 3 & 8) or IkB-alpha (Tyr-305) (lane 4 & 7) blocking peptides, or BSA conjugated to phospho-tyrosine (lane 5 & 9).")
![Western Blot: IkB-alpha Antibody (116) [NBP2-90043] - Lane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:37 kDaObserved band size:38 kDa](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-116-Western-Blot-NBP2-90043-img0001.jpg "Western Blot: IkB-alpha Antibody (116) [NBP2-90043] - Lane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:37 kDaObserved band size:38 kDa")
![Immunoprecipitation: IkB-alpha Antibody (116) [NBP2-90043] - Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg 293T Whole Cell LysateLane C:0.5 mg A431 Whole Cell LysateLane D:0.5 mg Hela Whole Cell LysateLane E:0.5 mg K562 Whole Cell Lysate2 uL anti-NFKBIA rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-NFKBIA rabbit monoclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 36 kDaObserved band size: 36 kDa](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-116-Immunoprecipitation-NBP2-90043-img0002.jpg)
![Western Blot: IkB-alpha [p Ser36] Antibody (JE56-65) [NBP3-32773] - Western blot analysis of IkB-alpha on different lysates with Rabbit anti-IkB-alpha antibody (NBP3-32773) at 1/1,000 dilution. Lane 1: C6 cell lysate Lane 2: C6 treated with 100n/mL Calyculin A for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 36 kDa Observed band size: 38 kDa Exposure time: 2 minutes 45 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32773) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32773_rabbit-ikb-alpha-p-ser36-mab-je56-65-288202412442912.jpg "Western Blot: IkB-alpha [p Ser36] Antibody (JE56-65) [NBP3-32773] - Western blot analysis of IkB-alpha on different lysates with Rabbit anti-IkB-alpha antibody (NBP3-32773) at 1/1,000 dilution. Lane 1: C6 cell lysate Lane 2: C6 treated with 100n/mL Calyculin A for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 36 kDa Observed band size: 38 kDa Exposure time: 2 minutes 45 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32773) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: IkB-alpha [p Ser 36] Antibody (SR1657) [NBP3-21594] - Western blot analysis using NBP3-21594 in HeLa cell lysate treated with Calyculin A.](http://images.novusbio.com/fullsize/nbp3-21594_rabbit-ikb-alpha-p-ser-36-mab-sr1657-57202316582171.jpg "Western Blot: IkB-alpha [p Ser 36] Antibody (SR1657) [NBP3-21594] - Western blot analysis using NBP3-21594 in HeLa cell lysate treated with Calyculin A.")
![Western Blot: IKB alpha Overexpression Lysate (Adult Normal) [NBP2-06636] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IKB alpha.](http://images.novusbio.com/fullsize/IKB-alpha-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-06636-img0001.jpg "Western Blot: IKB alpha Overexpression Lysate (Adult Normal) [NBP2-06636] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IKB alpha.")
![Western Blot: IkB-alpha [p Ser 32] Antibody (SR1656) [NBP3-21533] - Western blot analysis using NBP3-21533 in HeLa cell lysate treated with Calyculin A and TNF-alpha.](http://images.novusbio.com/fullsize/nbp3-21533_rabbit-ikb-alpha-p-ser-32-mab-sr1656-5720231754821.jpg "Western Blot: IkB-alpha [p Ser 32] Antibody (SR1656) [NBP3-21533] - Western blot analysis using NBP3-21533 in HeLa cell lysate treated with Calyculin A and TNF-alpha.")
![Recombinant Human IkB-alpha Protein [NBP1-72500]](http://images.novusbio.com/fullsize/Recombinant-Human-IkB-alpha-Protein-NBP1-72500-img0001.jpg "Recombinant Human IkB-alpha Protein [NBP1-72500]")
![Sandwich ELISA: IkB-alpha Antibody Pair [HRP] [NBP2-79383] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79383-img0001.jpg "Sandwich ELISA: IkB-alpha Antibody Pair [HRP] [NBP2-79383] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.")