| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB6218-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF6218-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83044-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97422-50ul | |||
![Western Blot: NEDD4 Antibody [NBP2-27197] - analysis of NEDD4 in mouse embryonic brain tissue lysate in the 1) absence and 2) presence of immunizing peptide using NEDD4 antibody at 5 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/NEDD4-Antibody-Western-Blot-NBP2-27197-img0001.jpg "Western Blot: NEDD4 Antibody [NBP2-27197] - analysis of NEDD4 in mouse embryonic brain tissue lysate in the 1) absence and 2) presence of immunizing peptide using NEDD4 antibody at 5 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Western Blot: NEDD4 Antibody [NBP2-27201] - Analysis of NEDD4 in mouse embryo brain tissue lysate in the 1) absence and 2) presence of immunizing peptide using NBP2-27201. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/NEDD4-Antibody-Western-Blot-NBP2-27201-img0001.jpg "Western Blot: NEDD4 Antibody [NBP2-27201] - Analysis of NEDD4 in mouse embryo brain tissue lysate in the 1) absence and 2) presence of immunizing peptide using NBP2-27201. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Western Blot: NEDD4 Antibody (S06-7C3) [NBP3-19815] - Western blot detection of Hela, A549, HL-60, U2OS, U87-MG cell lysates using NBP3-19815. Predicted band size: 149kDa. Observed band size: 115kDa.](http://images.novusbio.com/fullsize/nbp3-19815_rabbit-nedd4-mab-s06-7c3-10320252347261.jpg "Western Blot: NEDD4 Antibody (S06-7C3) [NBP3-19815] - Western blot detection of Hela, A549, HL-60, U2OS, U87-MG cell lysates using NBP3-19815. Predicted band size: 149kDa. Observed band size: 115kDa.")
![Western Blot: NEDD4 Antibody (JE35-58) [NBP3-32641] - Western blot analysis of NEDD4 on different lysates with Rabbit anti-NEDD4 antibody (NBP3-32641) at 1/2,000 dilution. Lane 1: U87MG cell lysate Lane 2: A549 cell lysate Lane 3: NIH/3T3 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 149 kDa Observed band size: 110 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32641) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32641_rabbit-nedd4-mab-je35-58-278202416331277.jpg "Western Blot: NEDD4 Antibody (JE35-58) [NBP3-32641] - Western blot analysis of NEDD4 on different lysates with Rabbit anti-NEDD4 antibody (NBP3-32641) at 1/2,000 dilution. Lane 1: U87MG cell lysate Lane 2: A549 cell lysate Lane 3: NIH/3T3 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 149 kDa Observed band size: 110 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32641) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: NEDD4 Antibody [NBP1-83044] - Staining of human cerebral cortex shows distinct cytoplasmic positivity in subsets of neuronal cells.](http://images.novusbio.com/fullsize/NEDD4-Antibody-Immunohistochemistry-Paraffin-NBP1-83044-img0002.jpg "Immunohistochemistry-Paraffin: NEDD4 Antibody [NBP1-83044] - Staining of human cerebral cortex shows distinct cytoplasmic positivity in subsets of neuronal cells.")
![Western Blot:[NBP3-30524]-Detection of human NEDD4 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa , Jurkat and HEK293T cells. Antibodies: Affinity purified rabbit anti-NEDD4 antibody used for WB at 0. 1 µg/ml and 1 µg/ml and used for IP at 6 µg/mg lysate. NEDD4 was also immunoprecipitated by rabbit anti-NEDD4 antibody , which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-30524_rabbit-nedd4-pab-23120251204059.jpg "Western Blot:[NBP3-30524]-Detection of human NEDD4 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa , Jurkat and HEK293T cells. Antibodies: Affinity purified rabbit anti-NEDD4 antibody used for WB at 0. 1 µg/ml and 1 µg/ml and used for IP at 6 µg/mg lysate. NEDD4 was also immunoprecipitated by rabbit anti-NEDD4 antibody , which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds.")
![Western Blot: NEDD4 Overexpression Lysate (Adult Normal) [NBP2-07658] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NEDD4.](http://images.novusbio.com/fullsize/NEDD4-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07658-img0001.jpg "Western Blot: NEDD4 Overexpression Lysate (Adult Normal) [NBP2-07658] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NEDD4.")
![Western Blot: NEDD4 Antibody (7R0M4) [NBP3-16398] - Western blot analysis of extracts of various cell lines, using NEDD4 Rabbit mAb (NBP3-16398) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/NEDD4-Antibody-7R0M4-Western-Blot-NBP3-16398-img0001.jpg "Western Blot: NEDD4 Antibody (7R0M4) [NBP3-16398] - Western blot analysis of extracts of various cell lines, using NEDD4 Rabbit mAb (NBP3-16398) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunoprecipitation:NEDD4 AntibodyNBP3-29418] -Detection of human NEDD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NEDD4 antibody NBP3-29418 (lot NBP3-29418-2) used for IP at 6 µg per reaction. NEDD4 was also immunoprecipitated by a previous lot of this antibody (lot NBP3-29418-1) and rabbit anti-NEDD4.. For blotting immunoprecipitated NEDD4, . was used at 1.0 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-29418_rabbit-nedd4-pab-301220241518243.jpg "Immunoprecipitation:NEDD4 AntibodyNBP3-29418] -Detection of human NEDD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NEDD4 antibody NBP3-29418 (lot NBP3-29418-2) used for IP at 6 µg per reaction. NEDD4 was also immunoprecipitated by a previous lot of this antibody (lot NBP3-29418-1) and rabbit anti-NEDD4.. For blotting immunoprecipitated NEDD4, . was used at 1.0 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry-Paraffin: NEDD4 Antibody [NBP2-97422] - Immunochemical staining of human NEDD4 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/NEDD4-Antibody-Immunohistochemistry-Paraffin-NBP2-97422-img0001.jpg "Immunohistochemistry-Paraffin: NEDD4 Antibody [NBP2-97422] - Immunochemical staining of human NEDD4 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.")