FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." alt="Serum starved U2OS cells treated with 100ng/mL PMA for 24 hrs (filled histogram) vs untreated U2OS cells (open histogram) were stained with Mouse Anti-Human MMP‑9 Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC9111F). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_thumb" />
909-MM) was serially diluted and captured by Rat Anti-Mouse Pro MMP‑9 Monoclonal Antibody (Catalog # MAB9092) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse Pro MMP‑9 Biotinylated Monoclonal Antibody (Catalog # ) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Mouse MMP‑9 (Catalog # 909-MM) was serially diluted and captured by Rat Anti-Mouse Pro MMP‑9 Monoclonal Antibody (Catalog # MAB9092) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse Pro MMP‑9 Biotinylated Monoclonal Antibody (Catalog # ) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />
