Note: Mouseover a species abbreviation on the product page to display the fullname.
How can we help you?
![Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody [NBP2-54656] - Analysis in human bone marrow and skeletal muscle tissues. Corresponding MKI67 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-Immunohistochemistry-Paraffin-NBP2-54656-img0004.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody [NBP2-54656] - Analysis in human bone marrow and skeletal muscle tissues. Corresponding MKI67 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody [NBP2-54656] - Staining of human cell line A-431 shows localization to nucleus & nucleoli. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-54656-img0003.jpg)
![Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody [NBP2-54656] - Staining of human bone marrow shows strong nuclear positivity in hematopoietic cells.](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-Immunohistochemistry-Paraffin-NBP2-54656-img0009.jpg)
![Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody [NBP2-54656] - Staining of human small intestine shows moderate to strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-Immunohistochemistry-Paraffin-NBP2-54656-img0005.jpg)
![Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody [NBP2-54656] - Staining of human skin shows moderate to string nuclear positivity in a subset of keratinocytes.](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-Immunohistochemistry-Paraffin-NBP2-54656-img0006.jpg)
![Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody [NBP2-54656] - Staining of human lymph node shows moderate to strong nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-Immunohistochemistry-Paraffin-NBP2-54656-img0007.jpg)
![Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody [NBP2-54656] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-Immunohistochemistry-Paraffin-NBP2-54656-img0008.jpg)
![Western Blot: Ki67/MKI67 Antibody [NBP3-05538] - Western blot analysis of equal amounts of cell lysates probed with Ki67/MKI67 Antibody at a dilution of 1:5,000, in green: [1] protein standard (red), [2] rapidly dividing HeLa cell cultures, [3] rapidly dividing HEK293 cell cultures. Strong double bands above 250kDa correspond to the two major Ki67/MKI67 isoforms of molecular weight 345kDa and 395kDa. Since Ki67/MKI67 is a rather unstable snd sjhort half life protein multiple proteolytic fragments of the two isoforms are also detected on the blot.](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-Western-Blot-NBP3-05538-img0002.jpg "Western Blot: Ki67/MKI67 Antibody [NBP3-05538] - Western blot analysis of equal amounts of cell lysates probed with Ki67/MKI67 Antibody at a dilution of 1:5,000, in green: [1] protein standard (red), [2] rapidly dividing HeLa cell cultures, [3] rapidly dividing HEK293 cell cultures. Strong double bands above 250kDa correspond to the two major Ki67/MKI67 isoforms of molecular weight 345kDa and 395kDa. Since Ki67/MKI67 is a rather unstable snd sjhort half life protein multiple proteolytic fragments of the two isoforms are also detected on the blot.")
![Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody [NBP3-05538] - Immunofluorescent analysis of HeLa cells stained with Ki67/MKI67 Antibody at a dilution of 1:2,000 in red, and mouse mAb to beta-tubulin at a dilution of 1:5,000 in green. The blue is DAPI staining of nuclear DNA. The Ki67/MKI67 antibody detects Ki67/MKI67 protein predominantly expressed in nucleoli of cells in interphase and in mitotic cells Ki67/MKI67 forms a coat around condensed chromosomes. Ki67/MKI67 is not detected in cells in the quiescent G0 state. The beta-tubulin antibody produces strong staining of cytoplasmic microtubules.](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05538-img0001.jpg)
![N/A Ki67/MKI67 [Biotin]](http://images.novusbio.com/fullsize2/DATA_Ki67_DY761705_ELISA_2510.jpg "N/A Ki67/MKI67 [Biotin]")
![Immunohistochemistry: Ki67/MKI67 Antibody (SR00-02) [NBP3-32498] - Immunofluorescence analysis of paraffin-embedded human lymph nodes tissue labeling Ki67/MKI67 with Rabbit anti-Ki67/MKI67 antibody (NBP3-32498) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32498, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32498_rabbit-ki67-mki67-mab-sr00-02-298202413585592.jpg "Immunohistochemistry: Ki67/MKI67 Antibody (SR00-02) [NBP3-32498] - Immunofluorescence analysis of paraffin-embedded human lymph nodes tissue labeling Ki67/MKI67 with Rabbit anti-Ki67/MKI67 antibody (NBP3-32498) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32498, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: Ki67/MKI67 Antibody (SR00-02) [NBP3-32498] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Ki67/MKI67 antibody (NBP3-32498) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32498) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32498_rabbit-ki67-mki67-mab-sr00-02-29820241435258.jpg)
![Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody (SR00-02) [NBP3-32498] - Immunocytochemistry analysis of HeLa cells labeling Ki67/MKI67 with Rabbit anti-Ki67/MKI67/MKI67 antibody (NBP3-32498) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ki67/MKI67 antibody (NBP3-32498) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32498_rabbit-ki67-mki67-mab-sr00-02-2920241192339.jpg)
![Immunohistochemistry: Ki67/MKI67 Antibody (PSH0-02) [NBP3-32499] - Immunohistochemical analysis of paraffin-embedded human cervix carcinoma tissue with Rabbit anti-Ki67/MKI67 antibody (NBP3-32499) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32499) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32499_rabbit-ki67-mki67-mab-psh0-02-298202414262672.jpg "Immunohistochemistry: Ki67/MKI67 Antibody (PSH0-02) [NBP3-32499] - Immunohistochemical analysis of paraffin-embedded human cervix carcinoma tissue with Rabbit anti-Ki67/MKI67 antibody (NBP3-32499) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32499) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Flow Cytometry: Ki67/MKI67 Antibody (PSH0-02) [NBP3-32499] - Flow cytometric analysis of A431 cells labeling Ki67/MKI67. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32499, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32499_rabbit-ki67-mki67-mab-psh0-02-29202416372190.jpg)
![Recombinant Human Ki67/MKI67 Protein [H00004288-Q01] - A specific band was detected for Ki67/MKI67 at approximately 36.74 kDa (as indicated). 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00004288-Q01-1.jpg "Recombinant Human Ki67/MKI67 Protein [H00004288-Q01] - A specific band was detected for Ki67/MKI67 at approximately 36.74 kDa (as indicated). 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody (rMKI67/8852) [NBP3-23761] - Formalin-fixed, paraffin-embedded human tonsil stained with Ki67/MKI67 Antibody (rMKI67/8852). Inset: PBS instead of primary antibody; secondary only negative control.](http://images.novusbio.com/fullsize/nbp3-23761_mouse-ki67-mki67-mab-rmki67-8852-2412025863694.jpg "Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody (rMKI67/8852) [NBP3-23761] - Formalin-fixed, paraffin-embedded human tonsil stained with Ki67/MKI67 Antibody (rMKI67/8852). Inset: PBS instead of primary antibody; secondary only negative control.")
![Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody (rMKI67/8852) [NBP3-23761] - Formalin-fixed, paraffin-embedded human tonsil stained with Ki67/MKI67 Antibody (rMKI67/8852). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/nbp3-23761_mouse-ki67-mki67-mab-rmki67-8852-2412025863684.jpg)
