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![Western Blot: IL-27 Antibody [NBP1-76698] - Recombinant Protein Validation. Loading: 30 ng of human recombinant IL-17(IL-17A+IL-17B) protein per lane. Antibodies: IL-27 NBP1-76698, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 ug/mL, Lane 2: 1 ug/mL, Lane 3: 2 ug/mL.](http://images.novusbio.com/fullsize/IL-27-Antibody-Western-Blot-NBP1-76698-img0005.jpg "<b>Expression of Tagged Proteins Validation. </b>Western Blot: IL-27 Antibody [NBP1-76698] - Recombinant Protein Validation. Loading: 30 ng of human recombinant IL-17(IL-17A+IL-17B) protein per lane. Antibodies: IL-27 NBP1-76698, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 ug/mL, Lane 2: 1 ug/mL, Lane 3: 2 ug/mL.")
![<b>Expression of Tagged Proteins Validation. </b>Western Blot: IL-27 Antibody [NBP1-76698] - Analysis of IL-27 in A-20 cell lysate with IL-27 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/IL-27-Antibody-Western-Blot-NBP1-76698-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: IL-27 Antibody [NBP1-76698] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines.Loading: 15 ug of lysates per lane. Antibodies: IL-27 NBP1-76698 (4 ug/mL), IL-27 NBP1-76412 (4 ug/mL), and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/IL-27-Antibody-Western-Blot-NBP1-76698-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: IL-27 Antibody - BSA Free [NBP1-76698] - Validation of IL-27 in Mouse EL4 Cells. Immunocytochemical analysis of Mouse EL4 cells using anti-IL-27 antibody at 10 ug/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76698_rabbit-polyclonal-il-27-antibody-2711202420212370.jpg)
![Western Blot: IL-27 Antibody [NBP2-16951] - Non-transfected (-) and IL27 -transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with IL27 antibody diluted by 1:1000.](http://images.novusbio.com/fullsize/IL-27-Antibody-Western-Blot-NBP2-16951-img0003.jpg "Western Blot: IL-27 Antibody [NBP2-16951] - Non-transfected (-) and IL27 -transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with IL27 antibody diluted by 1:1000.")
![Immunohistochemistry-Paraffin: IL27 Antibody [NBP2-16951] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/IL27-Antibody-Immunohistochemistry-Paraffin-NBP2-16951-img0002.jpg)
![ELISA: Mouse IL-27 ELISA Kit (Colorimetric) [NBP3-06785] - Samples were spiked with high concentrations of Mouse IL-27 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-06785_mouse-il-27-elisa-kit-colorimetric-132202416173433.jpg "ELISA: Mouse IL-27 ELISA Kit (Colorimetric) [NBP3-06785] - Samples were spiked with high concentrations of Mouse IL-27 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse IL-27 ELISA Kit (Colorimetric) [NBP3-06785] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-06785_mouse-il-27-elisa-kit-colorimetric-432024963972.jpg)
![ELISA: Human IL-27 ELISA Kit (Colorimetric) [NBP3-06784] - Samples were spiked with high concentrations of Human IL-27 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-06784_human-il-27-elisa-kit-colorimetric-132202416121375.jpg "ELISA: Human IL-27 ELISA Kit (Colorimetric) [NBP3-06784] - Samples were spiked with high concentrations of Human IL-27 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human IL-27 ELISA Kit (Colorimetric) [NBP3-06784] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-06784_human-il-27-elisa-kit-colorimetric-432024859781.jpg)
![ELISA: Rat IL-27 ELISA Kit (Colorimetric) [NBP3-06786] - Samples were spiked with high concentrations of Rat IL-27 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-06786_rat-il-27-elisa-kit-colorimetric-132202416143076.jpg "ELISA: Rat IL-27 ELISA Kit (Colorimetric) [NBP3-06786] - Samples were spiked with high concentrations of Rat IL-27 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat IL-27 ELISA Kit (Colorimetric) [NBP3-06786] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-06786_rat-il-27-elisa-kit-colorimetric-432024903510.jpg)
![Western Blot: IL27 Overexpression Lysate (Adult Normal) [NBL1-11947] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IL27.](http://images.novusbio.com/fullsize/IL27-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11947-img0002.jpg "Western Blot: IL27 Overexpression Lysate (Adult Normal) [NBL1-11947] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IL27.")