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![Western Blot: ISG15/UCRP Antibody (JE32-27) [NBP3-32484] - Western blot analysis of ISG15/UCRP on different lysates with Rabbit anti-ISG15/UCRP antibody (NBP3-32484) at 1/500 dilution. Lane 1: Hela whole cell lysate Lane 2: Hela treated with 10 ng/ml human IFN alpha 1 for 16 hours whole cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 18 kDa Observed band size: 13 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32484) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32484_rabbit-isg15-ucrp-mab-je32-27-278202415295574.jpg "Western Blot: ISG15/UCRP Antibody (JE32-27) [NBP3-32484] - Western blot analysis of ISG15/UCRP on different lysates with Rabbit anti-ISG15/UCRP antibody (NBP3-32484) at 1/500 dilution. Lane 1: Hela whole cell lysate Lane 2: Hela treated with 10 ng/ml human IFN alpha 1 for 16 hours whole cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 18 kDa Observed band size: 13 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32484) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![SDS-Page: Recombinant Human ISG15/UCRP Protein [NBC1-18442] - ISG15, 34kDa (308aa), confirmed by MALDI-TOF with a purity of 98% by SDS - PAGE](http://images.novusbio.com/fullsize/Interferon-induced-17-kDa-protein-Protein-SDS-Page-NBC1-18442-img0001.jpg "SDS-Page: Recombinant Human ISG15/UCRP Protein [NBC1-18442] - ISG15, 34kDa (308aa), confirmed by MALDI-TOF with a purity of 98% by SDS - PAGE")
![ELISA: Human ISG15/UCRP ELISA Kit (Colorimetric) [NBP3-38788] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-38788_human-isg15-ucrp-elisa-kit-colorimetric-211020241538053.png "ELISA: Human ISG15/UCRP ELISA Kit (Colorimetric) [NBP3-38788] - Standard Curve Reference")
![ELISA: Human ISG15/UCRP - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-38787] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-38787_human-isg15-ucrp-ready-to-use-elisa-kit-colorimetric-21102024153805.png "ELISA: Human ISG15/UCRP - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-38787] - Standard Curve Reference")
![Western Blot: ISG15/UCRP Antibody [NBP1-89809] - Analysis in control (vector only transfected HEK293T lysate) and ISG15 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/ISG15-UCRP-Antibody-Western-Blot-NBP1-89809-img0008.jpg "Western Blot: ISG15/UCRP Antibody [NBP1-89809] - Analysis in control (vector only transfected HEK293T lysate) and ISG15 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Western Blot: ISG15/UCRP Antibody [NBP1-89809] - Analysis in human cell line SK-MEL-30.](http://images.novusbio.com/fullsize/ISG15-UCRP-Antibody-Western-Blot-NBP1-89809-img0007.jpg)