| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1054AF405 | |||
| Alexa Fluor 488 | NB21-1054AF488 | |||
| Alexa Fluor 647 | NB21-1054AF647 | |||
| Alexa Fluor 700 | NB21-1054AF700 | |||
| Biotin | NB21-1054B | |||
| DyLight 405 | NB21-1054V | |||
| DyLight 488 | NB21-1054G | |||
| DyLight 550 | NB21-1054R | |||
| DyLight 650 | NB21-1054C | |||
| FITC | NB21-1054F | |||
| HRP | NB21-1054H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1054SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1252AF405 | |||
| Alexa Fluor 488 | NB21-1252AF488 | |||
| Alexa Fluor 647 | NB21-1252AF647 | |||
| Alexa Fluor 700 | NB21-1252AF700 | |||
| Biotin | NB21-1252B | |||
| DyLight 405 | NB21-1252V | |||
| DyLight 488 | NB21-1252G | |||
| DyLight 550 | NB21-1252R | |||
| DyLight 650 | NB21-1252C | |||
| FITC | NB21-1252F | |||
| HRP | NB21-1252H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1252SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1039AF405 | |||
| Alexa Fluor 488 | NB21-1039AF488 | |||
| Alexa Fluor 647 | NB21-1039AF647 | |||
| Alexa Fluor 700 | NB21-1039AF700 | |||
| Biotin | NB21-1039B | |||
| DyLight 405 | NB21-1039V | |||
| DyLight 488 | NB21-1039G | |||
| DyLight 550 | NB21-1039R | |||
| DyLight 650 | NB21-1039C | |||
| FITC | NB21-1039F | |||
| HRP | NB21-1039H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1039SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1071AF405 | |||
| Alexa Fluor 488 | NB21-1071AF488 | |||
| Alexa Fluor 647 | NB21-1071AF647 | |||
| Alexa Fluor 700 | NB21-1071AF700 | |||
| Biotin | NB21-1071B | |||
| DyLight 405 | NB21-1071V | |||
| DyLight 488 | NB21-1071G | |||
| DyLight 550 | NB21-1071R | |||
| DyLight 650 | NB21-1071C | |||
| FITC | NB21-1071F | |||
| HRP | NB21-1071H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1071SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1037AF405 | |||
| Alexa Fluor 488 | NB21-1037AF488 | |||
| Alexa Fluor 647 | NB21-1037AF647 | |||
| Alexa Fluor 700 | NB21-1037AF700 | |||
| Biotin | NB21-1037B | |||
| DyLight 405 | NB21-1037V | |||
| DyLight 488 | NB21-1037G | |||
| DyLight 550 | NB21-1037R | |||
| DyLight 650 | NB21-1037C | |||
| FITC | NB21-1037F | |||
| HRP | NB21-1037H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1037SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1141AF405 | |||
| Alexa Fluor 488 | NB21-1141AF488 | |||
| Alexa Fluor 647 | NB21-1141AF647 | |||
| Alexa Fluor 700 | NB21-1141AF700 | |||
| Biotin | NB21-1141B | |||
| DyLight 405 | NB21-1141V | |||
| DyLight 488 | NB21-1141G | |||
| DyLight 550 | NB21-1141R | |||
| DyLight 650 | NB21-1141C | |||
| FITC | NB21-1141F | |||
| HRP | NB21-1141H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1141SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1013AF405 | |||
| Alexa Fluor 488 | NB21-1013AF488 | |||
| Alexa Fluor 647 | NB21-1013AF647 | |||
| Alexa Fluor 700 | NB21-1013AF700 | |||
| Biotin | NB21-1013B | |||
| DyLight 405 | NB21-1013V | |||
| DyLight 488 | NB21-1013G | |||
| DyLight 550 | NB21-1013R | |||
| DyLight 650 | NB21-1013C | |||
| FITC | NB21-1013F | |||
| HRP | NB21-1013H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1013SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| DyLight 405 | NB21-1193V | |||
| FITC | NB21-1193F | |||
![Immunoprecipitation: Histone H3 [ac Lys27] Antibody-Histone H3 [ac Lys27] Antibody (2V10N10)-[NBP3-15933] -](http://images.novusbio.com/fullsize/nbp3-15933_rabbit-histone-h3-ac-lys-27-mab-2v10n10-immunoprecipitation-622024101825..jpg "Immunoprecipitation: Histone H3 [ac Lys27] Antibody-Histone H3 [ac Lys27] Antibody (2V10N10)-[NBP3-15933] -")
![Chromatin Immunoprecipitation: Histone H3 [ac Lys27] Antibody (2V10N10)- [NBP3-15933] - Analysis of extracts of HeLa cells, using Acetyl-Histone H3-K27 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-15933_rabbit-histone-h3-ac-lys-27-mab-2v10n10-chromatin-immunoprecipitation-622024102155..jpg)
![Dot Blot-Histone H3 [ac Lys27] Antibody (2V10N10)-NBP3-15933- Analysis of all sorts of peptides using Acetyl-Histone H3-K27 Rabbit mAb at 1:200000 dilution.](http://images.novusbio.com/fullsize/nbp3-15933_rabbit-histone-h3-ac-lys-27-mab-2v10n10-dot-blot-622024102348..jpg)
![<b>Biological Strategies Validation. </b>Western Blot: -Histone H3 [ac Lys27] Antibody (2V10N10)-NBP3-15933-analysis of lysates from C2C12 cells using Acetyl-Histone H3-K27 Rabbit mAb at 1:100000 dilution. C2C12 cells were treated by TSA (1 uM) at 37℃ for 18 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 20 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-15933_rabbit-histone-h3-ac-lys-27-mab-2v10n10-western-blot-622024102550..jpg)
![Immunohistochemistry: Histone H3 [ac Lys27] Antibody (2V10N10) [Histone H3] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using Histone H3 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15933_rabbit-histone-h3-ac-lys-27-mab-2v10n10-52202517213518.jpg)
![Immunohistochemistry: Histone H3 [ac Lys27] Antibody (2V10N10) [Histone H3] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Histone H3 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15933_rabbit-histone-h3-ac-lys-27-mab-2v10n10-5220251726021.jpg)
![Immunohistochemistry: Histone H3 [ac Lys27] Antibody (2V10N10) [Histone H3] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Histone H3 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15933_rabbit-histone-h3-ac-lys-27-mab-2v10n10-52202517192955.jpg)
![Immunohistochemistry: Histone H3 [ac Lys27] Antibody (2V10N10) [Histone H3] - Immunohistochemistry analysis of paraffin-embedded Human liver tissue using Histone H3 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15933_rabbit-histone-h3-ac-lys-27-mab-2v10n10-52202517284941.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys27] Antibody (2V10N10) [Histone H3] - Immunofluorescence analysis of HeLa treated with TSA and HeLa cells using Histone H3 Rabbit mAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15933_rabbit-histone-h3-ac-lys-27-mab-2v10n10-52202517284953.jpg)
![Immunohistochemistry: Histone H3 [ac Lys27] Antibody (2V10N10) [Histone H3] - Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using Histone H3 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15933_rabbit-histone-h3-ac-lys-27-mab-2v10n10-52202517202962.jpg)
![Immunohistochemistry: Histone H3 [ac Lys27] Antibody (2V10N10) [Histone H3] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using Histone H3 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15933_rabbit-histone-h3-ac-lys-27-mab-2v10n10-52202517223970.jpg)
![Immunohistochemistry: Histone H3 [ac Lys27] Antibody (2V10N10) [Histone H3] - Immunohistochemistry analysis of paraffin-embedded Human small intestine tissue using Histone H3 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15933_rabbit-histone-h3-ac-lys-27-mab-2v10n10-52202517273516.jpg)
![Immunohistochemistry: Histone H3 [ac Lys27] Antibody (2V10N10) [Histone H3] - Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using Histone H3 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15933_rabbit-histone-h3-ac-lys-27-mab-2v10n10-5220251723576.jpg)
![Dot Blot: Histone H3 [p Thr6, Trimethyl Lys9] Antibody [NB21-1054] - Analysis of H3K9me3/pT6 in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Trimethyl-Lys9]-Antibody-Dot-Blot-NB21-1054-img0010.jpg "Dot Blot: Histone H3 [p Thr6, Trimethyl Lys9] Antibody [NB21-1054] - Analysis of H3K9me3/pT6 in picomoles of peptide.")
![Chromatin Immunoprecipitation: Histone H3 [p Thr6, Trimethyl Lys9] Antibody [NB21-1054] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-1054 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Trimethyl-Lys9]-Antibody-Chromatin-Immunoprecipitation-NB21-1054-img0013.jpg)
![Western Blot: Histone H3 [p Thr6, Trimethyl Lys9] Antibody [NB21-1054] - Analysis of H3K9me3/pT6 in HeLa histone preps. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Trimethyl-Lys9]-Antibody-Western-Blot-NB21-1054-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [p Thr6, Trimethyl Lys9] Antibody [NB21-1054] - Histone H3.2 K9me3 pT6 antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Trimethyl-Lys9]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1054-img0016.jpg)
![Western Blot: Histone H3 [p Thr6, Trimethyl Lys9] Antibody [NB21-1054] - Analysis of H3K9Me3/pT6 in C. elegans embryo lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Trimethyl-Lys9]-Antibody-Western-Blot-NB21-1054-img0012.jpg)
![Dot Blot: Histone H3 [Dimethyl Lys36] Antibody [NB21-1252] - Analysis of H3 K36-Me2 using the peptides stated above in 10, 6, 3 and 1 picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys36]-Antibody-Dot-Blot-NB21-1252-img0010.jpg "Dot Blot: Histone H3 [Dimethyl Lys36] Antibody [NB21-1252] - Analysis of H3 K36-Me2 using the peptides stated above in 10, 6, 3 and 1 picomoles of peptide.")
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys36] Antibody [NB21-1252] - Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2 ug of NB21-1252 and 20 uL of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys36]-Antibody-Chromatin-Immunoprecipitation-NB21-1252-img0009.jpg)
![Western Blot: Histone H3 [Dimethyl Lys36] Antibody [NB21-1252] - Analysis of H3 K36Me2 in C. elegans embryo lysate. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys36]-Antibody-Western-Blot-NB21-1252-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Dimethyl Lys36] Antibody [NB21-1252] - Antibody was tested in Neuro2a cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys36]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1252-img0016.jpg)
![Western Blot: Histone H3 [Dimethyl Lys36] Antibody [NB21-1252] - Analysis of H3K36me2 in HeLa histone preps. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys36]-Antibody-Western-Blot-NB21-1252-img0011.jpg)
![Dot Blot: Histone H3 [p Thr6, Trimethyl Lys4] Antibody [NB21-1039] - Dot blot analysis of H3K4me3/pT6 with the peptides stated above in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Trimethyl-Lys4]-Antibody-Dot-Blot-NB21-1039-img0010.jpg "Dot Blot: Histone H3 [p Thr6, Trimethyl Lys4] Antibody [NB21-1039] - Dot blot analysis of H3K4me3/pT6 with the peptides stated above in picomoles of peptide.")
![Chromatin Immunoprecipitation: Histone H3 [p Thr6, Trimethyl Lys4] Antibody [NB21-1039] - 2 ug of NB21-1039 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control, DNA was measured by qRT-PCR and normalized to total input (input=1).](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Trimethyl-Lys4]-Antibody-Chromatin-Immunoprecipitation-NB21-1039-img0014.jpg)
![Western Blot: Histone H3 [p Thr6, Trimethyl Lys4] Antibody [NB21-1039] - WB analysis of H3K4Me3/pT6 in C. elegans embryo lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Trimethyl-Lys4]-Antibody-Western-Blot-NB21-1039-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [p Thr6, Trimethyl Lys4] Antibody [NB21-1039] - Histone H3.2 K4me3 pT6 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Trimethyl-Lys4]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1039-img0017.jpg)
![Western Blot: Histone H3 [p Thr6, Trimethyl Lys4] Antibody [NB21-1039] - WB detection of H3K4me3/pT6 in HeLa histone preps. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Trimethyl-Lys4]-Antibody-Western-Blot-NB21-1039-img0011.jpg)
![Dot Blot: Histone H3 [Monomethyl Lys9] Antibody [NB21-1071] - Analysis of H3K9me1 in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys9]-Antibody-Dot-Blot-NB21-1071-img0009.jpg "Dot Blot: Histone H3 [Monomethyl Lys9] Antibody [NB21-1071] - Analysis of H3K9me1 in picomoles of peptide.")
![Chromatin Immunoprecipitation: Histone H3 [Monomethyl Lys9] Antibody [NB21-1071] - Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2ug of NB21-1071 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys9]-Antibody-Chromatin-Immunoprecipitation-NB21-1071-img0011.jpg)
![Western Blot: Histone H3 [Monomethyl Lys9] Antibody [NB21-1071] - Analysis of H3K9me1 in NIH/3T3 histone preps. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys9]-Antibody-Western-Blot-NB21-1071-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Monomethyl Lys9] Antibody [NB21-1071] - Antibody at 1:50 in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys9]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1071-img0016.jpg)
![Western Blot: Histone H3 [Monomethyl Lys9] Antibody [NB21-1071] - WB analysis of H3K9me1 in C. elegans embryo lysate. Observed molecular weight is ~12 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys9]-Antibody-Western-Blot-NB21-1071-img0013.jpg)
![Western Blot: Histone H3 [ac Lys27] Antibody [NBP2-54615] - Histone 3 [Acetylated Lys27] [ac Lys27] Antibody [NBP2-54615] - Western Blot: Histone 3 [Acetylated Lys27] [ac Lys27] Antibody [NBP2-54615] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K27ac. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-3-[Acetylated-Lys27]-[ac-Lys27]-Antibody-Western-Blot-NBP2-54615-img0007.jpg "Western Blot: Histone H3 [ac Lys27] Antibody [NBP2-54615] - Histone 3 [Acetylated Lys27] [ac Lys27] Antibody [NBP2-54615] - Western Blot: Histone 3 [Acetylated Lys27] [ac Lys27] Antibody [NBP2-54615] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K27ac. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys27] Antibody [NBP2-54615] - HeLa cells were stained with the antibody against H3K27ac and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/ TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3K27ac antibody (top) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown at the bottom.](http://images.novusbio.com/fullsize/Histone-3-[Acetylated-Lys27]-[ac-Lys27]-Antibody-Immunofluorescence-NBP2-54615-img0006.jpg)
![Chromatin Immunoprecipitation: Histone H3 [ac Lys27] Antibody [NBP2-54615] - ChIP assays were performed using human K562 cells, the antibody against H3K27ac and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 100,000 cells. A titration consisting of 0.2, 0.5, 1 and 2 ug of antibody per ChIP experiment was analyzed. IgG (1 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active GAPDH and EIF4A2 genes, used as positive controls, and for the coding regions of the inactive MB and MYT1 genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis)](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys27-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-54615-img0010.jpg)
![Chromatin Immunoprecipitation: Histone H3 [ac Lys27] Antibody [NBP2-54615] - ChIP assays were performed using human HeLa cells, the antibody against H3K27ac and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 1,000,000 cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active EIF4A2 and ACTB genes, used as positive controls, and for the inactive TSH2B and MYT1 genes, used as negative controls.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys27-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-54615-img0009.jpg)
![Dot Blot: Histone H3 [ac Lys27] Antibody [NBP2-54615] - Histone 3 [Acetylated Lys27] [ac Lys27] Antibody [NBP2-54615] - Dot Blot: Histone 3 [Acetylated Lys27] [ac Lys27] Antibody [NBP2-54615] - To test the cross reactivity of the antibody against H3K27ac, a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3K27. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-3-[Acetylated-Lys27]-[ac-Lys27]-Antibody-Dot-Blot-NBP2-54615-img0008.jpg)
![ELISA: Histone H3 [ac Lys27] Antibody [NBP2-54615] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against H3K27ac The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:8,300.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys27]-Antibody-ELISA-NBP2-54615-img0011.jpg)
![Western Blot: Histone H3 [Trimethyl Lys4] Antibody [NBP2-54610] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K4me3. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-3-[Tryimethyl-Lys4]-Antibody-Western-Blot-NBP2-54610-img0003.jpg "Western Blot: Histone H3 [Trimethyl Lys4] Antibody [NBP2-54610] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K4me3. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.")
![Immunohistochemistry: Histone H3 [Trimethyl Lys4] Antibody [NBP2-54610] - Histone 3 [Tryimethyl Lys4] Antibody [NBP2-54610] - HeLa cells were stained with the antibody against H3K4me3 and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3K4me3 antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-3-[Tryimethyl-Lys4]-Antibody-Immunohistochemistry-NBP2-54610-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys4] Antibody [NBP2-54610] - Histone 3 [Tryimethyl Lys4] [Trimethyl Lys4] Antibody [NBP2-54610] - ChIP assays were performed using human HeLa cells, the antibody against H3K4me3, and optimized PCR primer pairs for qPCR. Figure shows the recovery, expressed as a percent of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that trimethylation of K4 at histone H3 is associated with the promoters of active genes.](http://images.novusbio.com/fullsize/Histone-3-Tryimethyl-Lys4-Trimethyl-Lys4-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-54610-img0005.jpg)
![Dot Blot: Histone H3 [Trimethyl Lys4] Antibody [NBP2-54610] - Histone 3 [Tryimethyl Lys4] Antibody [NBP2-54610] - One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:10,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-3-[Tryimethyl-Lys4]-Antibody-Dot-Blot-NBP2-54610-img0002.jpg)
![ELISA: Histone H3 [Trimethyl Lys4] Antibody [NBP2-54610] - Histone 3 [Tryimethyl Lys4] [Trimethyl Lys4] Antibody [NBP2-54610] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against H3K4me3. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:18,350.](http://images.novusbio.com/fullsize/Histone-3-[Tryimethyl-Lys4]-[Trimethyl-Lys4]-Antibody-ELISA-NBP2-54610-img0006.jpg)
![Protein Array: Histone H3 [Trimethyl Lys4] Antibody [NBP2-54610] - Histone 3 [Tryimethyl Lys4] [Trimethyl Lys4] Antibody [NBP2-54610] - The specificity of the antibody was further demonstrated by peptide array analyses on an array containing 384 peptides with different combinations of modifications from histone H3, H4, H2A and H2B. The antibody was used at a dilution of 1:10,000. Figure shows a high specificity for the peptides containing the H3K4me3 modification.](http://images.novusbio.com/fullsize/Histone-3-[Tryimethyl-Lys4]-[Trimethyl-Lys4]-Antibody-Protein-Array-NBP2-54610-img0007.jpg)
![Western Blot: Histone H3 [ac Lys14, ac Lys9] Antibody [NBP2-59181] - Histone extracts of HeLa cells (15 ug) were analysed by Western blot using the antibody directed against H3K9/14ac diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys14--ac-Lys9]-Antibody-Western-Blot-NBP2-59181-img0001.jpg "Western Blot: Histone H3 [ac Lys14, ac Lys9] Antibody [NBP2-59181] - Histone extracts of HeLa cells (15 ug) were analysed by Western blot using the antibody directed against H3K9/14ac diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [ac Lys14, ac Lys9] Antibody [NBP2-59181] - ChIP assays were performed using HeLa cells, the antibody against H3K9/14ac and optimized primer pairs for qPCR. ChIP was performed with a ChIP kit, using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 ug per ChIP experiment was analysed. IgG (5 ug/IP) was used as negative IP control.QPCR was performed using primers specific for the promoter of the ACTB gene as a positive control target and for exon 2 of the MB gene as a negative control target. Figure shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA). These results confirm the observation that acetylation of H3K9/14 is present at active promoters.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys14--ac-Lys9]-Antibody-Chromatin-Immunoprecipitation-NBP2-59181-img0008.jpg)
![Chromatin Immunoprecipitation: Histone H3 [ac Lys14, ac Lys9] Antibody [NBP2-59181] - ChIP assays were performed using HeLa cells, the antibody against H3K9/14ac and optimized primer pairs for qPCR. ChIP was performed using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 ug per ChIP experiment was analysed. IgG (5 ug/IP) was used as negative IP control. QPCR was performed using primers specific for the promoter of the ACTB gene as a positive control target and for exon 2 of the MB gene as a negative control target. Figure shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA). These results confirm the observation that acetylation of H3K9/14 is present at active promoters.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys14--ac-Lys9]-Antibody-Chromatin-Immunoprecipitation-NBP2-59181-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H3 [ac Lys14, ac Lys9] Antibody [NBP2-59181] - ChIP was performed with 1 ug of the antibody against H3K9/14ac on sheared chromatin from 1 million HeLaS3 cells. IgG (2 ug/IP) was used as a negative IP control. The IP'd DNA was analysed by QPCR with optimized PCR primer pairs for the promoters of the active GAPDH and c-fos genes, used as positive control targets, and the coding region of the inactive MB gene and the Sat2 satellite repeat, used as negative control targets.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys14--ac-Lys9]-Antibody-Chromatin-Immunoprecipitation-NBP2-59181-img0005.jpg)
![Dot Blot: Histone H3 [ac Lys14, ac Lys9] Antibody [NBP2-59181] - A Dot Blot analysis was performed to test the cross reactivity of the antibody against H3K9/14ac with peptides containing other histone modifications and the unmodified H3K9/14 sequence. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys14--ac-Lys9]-Antibody-Dot-Blot-NBP2-59181-img0002.jpg)
![ELISA: Histone H3 [ac Lys14, ac Lys9] Antibody [NBP2-59181] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H3K9/14ac, crude serum and flow through in antigen coated wells. The antigen used was a peptie containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1:5,900.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys14--ac-Lys9]-Antibody-ELISA-NBP2-59181-img0003.jpg)
![Immunofluorescence: Histone H3 [ac Lys14, ac Lys9] Antibody [NBP2-59181] - Mouse NIH3T3 cells were stained with the antibody against H3K9/14ac and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K9/14ac antibody (left) diluted 1:500 in blocking solution followed by an anti- rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys14-ac-Lys9]-Antibody-Immunofluorescence-NBP2-59181-img0006.jpg)
![Western Blot: Histone H3 [Monomethyl Lys4, p Thr6] Antibody [NB21-1037] - Aanalysis of H3K4me1/pt6 in C. elegans embryonic lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys4-p-Thr6]-Antibody-Western-Blot-NB21-1037-img0004.jpg "Western Blot: Histone H3 [Monomethyl Lys4, p Thr6] Antibody [NB21-1037] - Aanalysis of H3K4me1/pt6 in C. elegans embryonic lysate. Observed molecular weight is ~15 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Monomethyl Lys4, p Thr6] Antibody [NB21-1037] - Staining of H3K4me1/pT6 in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys4--p-Thr6]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1037-img0007.jpg)
![Western Blot: Histone H3 [Monomethyl Lys4, p Thr6] Antibody [NB21-1037] - Detection of H3K4me1/pT6 in HeLa histone preps. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys4-p-Thr6]-Antibody-Western-Blot-NB21-1037-img0001.jpg)
![Dot Blot: Histone H3 [Monomethyl Lys4, p Thr6] Antibody - BSA Free [NB21-1037] - Analysis of H3K4me1/pT6 antibody in picomoles of protein.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys4--p-Thr6]-Antibody---BSA-Free-Dot-Blot-NB21-1037-img0008.jpg)
![Dot Blot: Histone H3 [Monomethyl Lys18] Antibody [NB21-1141] - Analysis of HIstone H3K18me1 in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys18]-Antibody-Dot-Blot-NB21-1141-img0006.jpg "Dot Blot: Histone H3 [Monomethyl Lys18] Antibody [NB21-1141] - Analysis of HIstone H3K18me1 in picomoles of peptide.")
![Western Blot: Histone H3 [Monomethyl Lys18] Antibody [NB21-1141] - Detection of H3K18me1 in HeLa histone prep lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys18]-Antibody-Western-Blot-NB21-1141-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Monomethyl Lys18] Antibody [NB21-1141] - Antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys18]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1141-img0011.jpg)
![Western Blot: Histone H3 [Monomethyl Lys18] Antibody [NB21-1141] - Analysis of Histone H3K18me1 in C. elegans embryo lysate. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys18]-Antibody-Western-Blot-NB21-1141-img0007.jpg)
![Dot Blot: Histone H3 [p Thr3, Dimethyl Lys4] Antibody [NB21-1013] - Dot Blot analysis of H3pT3/K4me2 using the peptides listed above in 100, 10 & 1 picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr3--Dimethyl-Lys4]-Antibody-Dot-Blot-NB21-1013-img0005.jpg "Dot Blot: Histone H3 [p Thr3, Dimethyl Lys4] Antibody [NB21-1013] - Dot Blot analysis of H3pT3/K4me2 using the peptides listed above in 100, 10 & 1 picomoles of peptide.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [p Thr3, Dimethyl Lys4] Antibody [NB21-1013] - Histone H3 pT3/K4me2 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr3--Dimethyl-Lys4]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1013-img0012.jpg)
![Chromatin Immunoprecipitation: Histone H3 [p Thr3, Dimethyl Lys4] Antibody [NB21-1013] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-1013 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr3--Dimethyl-Lys4]-Antibody-Chromatin-Immunoprecipitation-NB21-1013-img0010.jpg)
![Western Blot: Histone H3 [p Thr3, Dimethyl Lys4] Antibody [NB21-1013] - WB analysis of Histone H3 pT3/K4me2 in NIH-3T3 histone prep lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr3--Dimethyl-Lys4]-Antibody-Western-Blot-NB21-1013-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [p Ser28, Trimethyl Lys27] Antibody [NB21-1193] - Histone H3 K27me3/pS28 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Histone-H3-[p-Ser28--Trimethyl-Lys27]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1193-img0016.jpg "Immunocytochemistry/Immunofluorescence: Histone H3 [p Ser28, Trimethyl Lys27] Antibody [NB21-1193] - Histone H3 K27me3/pS28 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).")
![Dot Blot: Histone H3 [p Ser28, Trimethyl Lys27] Antibody [NB21-1193] - Analysis of NB21-1193 using the peptides listed above in 100, 10, and 1 picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[p-Ser28--Trimethyl-Lys27]-Antibody-Dot-Blot-NB21-1193-img0006.jpg)
![Chromatin Immunoprecipitation: Histone H3 [p Ser28, Trimethyl Lys27] Antibody [NB21-1193] - Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2ug of NB21-1193 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H3-[p-Ser28--Trimethyl-Lys27]-Antibody-Chromatin-Immunoprecipitation-NB21-1193-img0010.jpg)
![Western Blot: Histone H3 [p Ser28, Trimethyl Lys27] Antibody [NB21-1193] - Tested in (1) HeLa histone prep (2) NIH-3T3 histone prep and (3) C. elegans embryo cell lysate. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Ser28--Trimethyl-Lys27]-Antibody-Western-Blot-NB21-1193-img0013.jpg)