Fluorescent ICC Staining of Cells on Coverslips." alt="GSK‑3 beta was detected in immersion fixed MCF-7 human breast cancer cell line using 10 µg/mL Human GSK‑3 beta Monoclonal Antibody (Catalog # MAB25061) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB25061-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26422-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21576-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-02983-20ul | |||
![Immunohistochemistry: GSK-3 beta [p Ser9] Antibody (3A6) [NBP3-26422] - Image of GSK-3 beta [p Ser9] Antibody (3A6) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26422_rabbit-gsk-3-beta-p-ser-9-mab-3a6-26220241525330.jpg "Immunohistochemistry: GSK-3 beta [p Ser9] Antibody (3A6) [NBP3-26422] - Image of GSK-3 beta [p Ser9] Antibody (3A6) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![<b>Biological Strategies Validation. </b>Western Blot: GSK-3 beta [p Ser9] Antibody (3A6) [NBP3-26422] - Positive Western Blot detected in Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate (treated with Calyculin A or not).<br /> All lanes: GSK-3 beta [p Ser9] Antibody at 0.77ug/ml.<br /> Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.<br /> Predicted band size: 46 KDa<br /> Observed band size: 46 KDa<br />](http://images.novusbio.com/fullsize/nbp3-26422_rabbit-gsk-3-beta-p-ser-9-mab-3a6-28220241136300.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: GSK-3 beta [p Ser9] Antibody (3A6) [NBP3-26422] - Staining of Hela cells(treated with 50mM Calyculin A for 30min) with GSK-3 beta [p Ser9] Antibody (3A6) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26422_rabbit-gsk-3-beta-p-ser-9-mab-3a6-28220241312216.jpg)
![Immunohistochemistry: GSK-3 beta [p Ser 9] Antibody (SR1801) [NBP3-21576] - Immunohistochemical analysis of paraffin-embedded human kidney, using NBP3-21576.](http://images.novusbio.com/fullsize/nbp3-21576_rabbit-gsk-3-beta-p-ser-9-mab-sr1801-5720231526118.jpg "Immunohistochemistry: GSK-3 beta [p Ser 9] Antibody (SR1801) [NBP3-21576] - Immunohistochemical analysis of paraffin-embedded human kidney, using NBP3-21576.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: GSK-3 beta [p Ser 9] Antibody (SR1801) [NBP3-21576] - Immunofluorescent analysis of HeLa cells treated with Calyculin A, using NBP3-21576.](http://images.novusbio.com/fullsize/nbp3-21576_rabbit-gsk-3-beta-p-ser-9-mab-sr1801-57202316494217.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: GSK-3 beta [p Ser 9] Antibody (SR1801) [NBP3-21576] - Western blot analysis using NBP3-21576 in 293T cell lysates, treated with Calyculin A.](http://images.novusbio.com/fullsize/nbp3-21576_rabbit-gsk-3-beta-p-ser-9-mab-sr1801-5720231754890.jpg)
![Western Blot: GSK-3 beta Antibody [NBP3-02983] - Analysis of extracts of various cell lines, using GSK-3 beta antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Kit.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-Western-Blot-NBP3-02983-img0004.jpg "Western Blot: GSK-3 beta Antibody [NBP3-02983] - Analysis of extracts of various cell lines, using GSK-3 beta antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Kit.")
![Western Blot: GSK-3 beta Antibody [NBP3-02983] - Analysis of 200ug extracts of 22Rv1 cells, using GSK-3 beta antibody . Western blot was performed from the immunoprecipitate using this antibody.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-Western-Blot-NBP3-02983-img0002.jpg)
![Western Blot: GSK-3 beta [p Ser390] Antibody [NBP3-13303] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK-3 beta (phospho Thr390) antibody (NBP3-13303) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GSK-3-beta-[p-Ser390]-Antibody-Western-Blot-NBP3-13303-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: GSK-3 beta [p Ser390] Antibody [NBP3-13303] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK-3 beta (phospho Thr390) antibody (NBP3-13303) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: GSK3 beta Overexpression Lysate (Adult Normal) [NBL1-11362] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GSK3 beta.](http://images.novusbio.com/fullsize/GSK3-beta-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11362-img0001.jpg "Western Blot: GSK3 beta Overexpression Lysate (Adult Normal) [NBL1-11362] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GSK3 beta.")
