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![Immunohistochemistry: BMI-1 Antibody (1F2) [NBP3-26477] - Image of BMI-1 Antibody (1F2) diluted at 1:100 and staining in paraffin-embedded human liver cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26477_rabbit-bmi-1-mab-1f2-27220248453210.jpg "Immunohistochemistry: BMI-1 Antibody (1F2) [NBP3-26477] - Image of BMI-1 Antibody (1F2) diluted at 1:100 and staining in paraffin-embedded human liver cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: BMI-1 Antibody (1F2) [NBP3-26477] - Image of BMI-1 Antibody (1F2) diluted at 1:100 and staining in paraffin-embedded human liver cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26477_rabbit-bmi-1-mab-1f2-27220248274914.jpg)
![Western Blot: BMI-1 Antibody (1F2) [NBP3-26477] - Positive Western Blot detected in: Hela whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, THP-1 whole cell lysate, U87 whole cell lysate, U251 whole cell lysate. All lanes: BMI-1 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 37 kDa Observed band size: 45 kDa](http://images.novusbio.com/fullsize/nbp3-26477_rabbit-bmi-1-mab-1f2-282202411485821.jpg)
![Western Blot: BMI-1 Antibody [NB600-487] - Analysis of a band 37 kDa corresponding to human Bmi1 (arrowhead). Approximately 20 ug of a U2OS whole cell lysate (bone osteosarcoma) was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking in PBS containing 5% nonfat dry milk, the membrane was probed overnight at 4 C with the primary antibody diluted to 1:1,000 in PBS containing 1% nonfat dry milk. The membrane was washed and reacted with a 1:20,000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] MX for 45 min at room temperature.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NB600-487-img0007.jpg "Western Blot: BMI-1 Antibody [NB600-487] - Analysis of a band 37 kDa corresponding to human Bmi1 (arrowhead). Approximately 20 ug of a U2OS whole cell lysate (bone osteosarcoma) was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking in PBS containing 5% nonfat dry milk, the membrane was probed overnight at 4 C with the primary antibody diluted to 1:1,000 in PBS containing 1% nonfat dry milk. The membrane was washed and reacted with a 1:20,000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] MX for 45 min at room temperature.")
![Immunocytochemistry/Immunofluorescence: BMI-1 Antibody [NB600-487] - Nuclear staining (green) of methanol fixed (100%, 5 min) HepG2 cells. The cells were blocked and permeabilized in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h prior to incubation with the primary antibody (1:200 dilution) overnight at +4C and detected with a 488nm fluorescent dye conjugated secondary Ab. Cell nuclei are stained with DAPI (blue) and plasma membranes are stained with WGA (red).](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunocytochemistry-Immunofluorescence-NB600-487-img0005.jpg)
![SDS-Page: Recombinant Human BMI-1 Protein [H00000648-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-BMI-1-Protein-SDS-Page-H00000648-P01-img0001.jpg "SDS-Page: Recombinant Human BMI-1 Protein [H00000648-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: BMI-1 Antibody (6H9B0) [NBP3-15562] - Western blot analysis of extracts of various cell lines, using BMI-1 antibody (NBP3-15562) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/BMI-1-Antibody-6H9B0-Western-Blot-NBP3-15562-img0001.jpg "Western Blot: BMI-1 Antibody (6H9B0) [NBP3-15562] - Western blot analysis of extracts of various cell lines, using BMI-1 antibody (NBP3-15562) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Western Blot: BMI-1 Antibody (S08-7A6) [NBP3-19839] - Western blot detection of BMI-1 in C6 (Lane 1), Rat Brain (Lane 2), Ramos (Lane 3)using NBP3-19839 (1:1000 diluted)](http://images.novusbio.com/fullsize/BMI-1-Antibody-S08-7A6-Western-Blot-NBP3-19839-img0001.jpg "Western Blot: BMI-1 Antibody (S08-7A6) [NBP3-19839] - Western blot detection of BMI-1 in C6 (Lane 1), Rat Brain (Lane 2), Ramos (Lane 3)using NBP3-19839 (1:1000 diluted)")
![Immunocytochemistry/Immunofluorescence: BMI-1 Antibody [NBP2-57552] - Staining of human cell line HEK 293 shows localization to nuclear bodies.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57552-img0001.jpg "Immunocytochemistry/Immunofluorescence: BMI-1 Antibody [NBP2-57552] - Staining of human cell line HEK 293 shows localization to nuclear bodies.")
![Western Blot: Bmi1 Overexpression Lysate (Adult Normal) [NBL1-07997] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Bmi1.](http://images.novusbio.com/fullsize/Bmi1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07997-img0002.jpg "Western Blot: Bmi1 Overexpression Lysate (Adult Normal) [NBL1-07997] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Bmi1.")