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![SDS-Page: Recombinant Human Beclin 1 Protein [H00008678-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-Beclin-1-Protein-SDS-Page-H00008678-P01-img0001.jpg "SDS-Page: Recombinant Human Beclin 1 Protein [H00008678-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Immunohistochemistry: Beclin 1 Antibody (JE59-31) [NBP3-32076] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Beclin 1 antibody (NBP3-32076) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32076) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32076_rabbit-beclin-1-mab-je59-31-29820241435222.jpg "Immunohistochemistry: Beclin 1 Antibody (JE59-31) [NBP3-32076] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Beclin 1 antibody (NBP3-32076) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32076) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: Beclin 1 Antibody (JE59-31) [NBP3-32076] - Immunocytochemistry analysis of Raji cells labeling Beclin 1 with Rabbit anti-Beclin 1 antibody (NBP3-32076) at 1/250 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Beclin 1 antibody (NBP3-32076) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32076_rabbit-beclin-1-mab-je59-31-29202410484858.jpg)
![Western Blot: Beclin 1/ATG6 [p Ser234] Antibody [NBP2-29654] - Western Blot: Beclin 1/ATG6 [p Ser234] Antibody [NBP2-29654] - Beclin 1 [p Ser234] Antibody [NBP2-29654] - flag-beclin-1 WT immunoprecipitates expressed in 293T cells showing specific immunolabeling of beclin-1 when phosphorylated at S234 (WT). This immunolabeling is decreased in the beclin-1 double alanine substitution mutant (S234A, S295A).](http://images.novusbio.com/fullsize/Beclin-1-ATG6-[p-Ser234]-Antibody-Western-Blot-NBP2-29654-img0004.jpg "Western Blot: Beclin 1/ATG6 [p Ser234] Antibody [NBP2-29654] - Western Blot: Beclin 1/ATG6 [p Ser234] Antibody [NBP2-29654] - Beclin 1 [p Ser234] Antibody [NBP2-29654] - flag-beclin-1 WT immunoprecipitates expressed in 293T cells showing specific immunolabeling of beclin-1 when phosphorylated at S234 (WT). This immunolabeling is decreased in the beclin-1 double alanine substitution mutant (S234A, S295A).")
![ELISA: Rat Beclin 1 ELISA Kit (Colorimetric) [NBP2-69960] - Samples were spiked with high concentrations of Rat Beclin 1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-69960_rat-beclin-1-elisa-kit-colorimetric-13220241692216.jpg "ELISA: Rat Beclin 1 ELISA Kit (Colorimetric) [NBP2-69960] - Samples were spiked with high concentrations of Rat Beclin 1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Beclin 1 ELISA Kit (Colorimetric) [NBP2-69960] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-69960_rat-beclin-1-elisa-kit-colorimetric-4320249115629.jpg)
![Western Blot: Beclin 1 [p Ser15] Antibody [NBP3-16954] - Analysis of extracts of various cell lines, using Beclin 1 antibody (NBP3-16954) at 1:500 dilution.NIH/3T3 and C6 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Beclin-1-[p-Ser15]-Antibody-Western-Blot-NBP3-16954-img0001.jpg "Western Blot: Beclin 1 [p Ser15] Antibody [NBP3-16954] - Analysis of extracts of various cell lines, using Beclin 1 antibody (NBP3-16954) at 1:500 dilution.NIH/3T3 and C6 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: Beclin 1 Antibody [NBP2-92980] - Western blot analysis of extracts of various cell lines, using Beclin 1 antibody (NBP2-92980) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Beclin 1 Antibody-Western Blot-NBP2-92980-img0002.jpg "Western Blot: Beclin 1 Antibody [NBP2-92980] - Western blot analysis of extracts of various cell lines, using Beclin 1 antibody (NBP2-92980) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Western Blot: Beclin 1 [p Ser90] Antibody [NBP3-13298] - Untreated (-) and treated (+) MCF-7 whole cell extracts (60 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Beclin1 (phospho Ser90) antibody (NBP3-13298) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Beclin-1-[p-Ser90]-Antibody-Western-Blot-NBP3-13298-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Beclin 1 [p Ser90] Antibody [NBP3-13298] - Untreated (-) and treated (+) MCF-7 whole cell extracts (60 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Beclin1 (phospho Ser90) antibody (NBP3-13298) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![SDS-Page: Recombinant Human Beclin 1 Protein [NBP2-22710] -](http://images.novusbio.com/fullsize/Beclin-2-Protein-SDS-Page-NBP2-22710-img0001.jpg "SDS-Page: Recombinant Human Beclin 1 Protein [NBP2-22710] -")
![ELISA: Human Beclin 1 ELISA Kit (Colorimetric) [NBP2-69959] - Samples were spiked with high concentrations of Human Beclin 1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-69959_human-beclin-1-elisa-kit-colorimetric-13220241621536.jpg "ELISA: Human Beclin 1 ELISA Kit (Colorimetric) [NBP2-69959] - Samples were spiked with high concentrations of Human Beclin 1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Beclin 1 ELISA Kit (Colorimetric) [NBP2-69959] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-69959_human-beclin-1-elisa-kit-colorimetric-432024973627.jpg)
![Western Blot: Beclin 1 Overexpression Lysate (Adult Normal) [NBL1-07966] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Beclin 1.](http://images.novusbio.com/fullsize/Beclin-1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07966-img0002.jpg "Western Blot: Beclin 1 Overexpression Lysate (Adult Normal) [NBL1-07966] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Beclin 1.")