| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50101-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21562-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21567-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21568-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43824 | |||
| BSA Free | NBP2-52957-25ul | |||
![Western Blot: AKT1/2/3 [p Ser473] Antibody (2E3A3) [NBP3-16948] - Analysis of extracts of various cell lines, using Phospho-Akt-S473 antibody (NBP3-16948) at 1:1000 dilution.Jurkat cells and C6 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/AKT1-2-3-p-Ser473-Antibody-2E3A3-Western-Blot-NBP3-16948-img0001.jpg "Western Blot: AKT1/2/3 [p Ser473] Antibody (2E3A3) [NBP3-16948] - Analysis of extracts of various cell lines, using Phospho-Akt-S473 antibody (NBP3-16948) at 1:1000 dilution.Jurkat cells and C6 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: AKT1/2/3 [p Ser473] Antibody (2E3A3) [NBP3-16948] - Analysis of extracts of NIH/3T3 cells, using Phospho-Akt-S473 antibody (NBP3-16948) at 1:1000 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/AKT1-2-3-p-Ser473-Antibody-2E3A3-Western-Blot-NBP3-16948-img0002.jpg)
![Immunohistochemistry: AKT1 Antibody (V7) [NBP2-50101] - IHC staining of zebrafish skin using AKT1 antibody. AKT1 can be seen to localise in the cytoplasm.](http://images.novusbio.com/fullsize/Akt1-Antibody-V7-Immunohistochemistry-NBP2-50101-img0001.jpg "Immunohistochemistry: AKT1 Antibody (V7) [NBP2-50101] - IHC staining of zebrafish skin using AKT1 antibody. AKT1 can be seen to localise in the cytoplasm.")
![Western Blot: AKT1 [p Thr 450] Antibody (SR2048) [NBP3-21562] - Western blot analysis using NBP3-21562 in (1) 3T3 cell lysate treated with Lambda Phosphatase; (2) 3T3 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21562_rabbit-akt1-p-thr-450-mab-sr2048-57202316582143.jpg "Western Blot: AKT1 [p Thr 450] Antibody (SR2048) [NBP3-21562] - Western blot analysis using NBP3-21562 in (1) 3T3 cell lysate treated with Lambda Phosphatase; (2) 3T3 cell lysate.")
![Western Blot: AKT1 [p Ser129] Antibody [NBP3-13418] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT1 (phospho Ser129) antibody (NBP3-13418) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/AKT1-[p-Ser129]-Antibody-Western-Blot-NBP3-13418-img0002.jpg "Western Blot: AKT1 [p Ser129] Antibody [NBP3-13418] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT1 (phospho Ser129) antibody (NBP3-13418) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Biological Strategies Validation. </b>Western Blot: AKT1 [p Ser129] Antibody [NBP3-13418] - Untreated (-) and treated (+) Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT1 (phospho Ser129) antibody (NBP3-13418) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/AKT1-[p-Ser129]-Antibody-Western-Blot-NBP3-13418-img0001.jpg)
![Western Blot: AKT1 [p Ser 129] Antibody (SR2047) [NBP3-21567] - Western blot analysis using NBP3-21567 in (1) MCF-7 cell lysate treated with Alkaline Phosphatase; (2) MCF-7 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21567_rabbit-akt1-p-ser-129-mab-sr2047-5720231754841.jpg "Western Blot: AKT1 [p Ser 129] Antibody (SR2047) [NBP3-21567] - Western blot analysis using NBP3-21567 in (1) MCF-7 cell lysate treated with Alkaline Phosphatase; (2) MCF-7 cell lysate.")
![Western Blot: AKT1 [p Ser 124] Antibody (SR2049) [NBP3-21568] - Western blot analysis using NBP3-21568 in (1) MCF-7 cell treated with Alkaline Phosphatase lysate; (2) MCF-7 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21568_rabbit-akt1-p-ser-124-mab-sr2049-67202310623.jpg "Western Blot: AKT1 [p Ser 124] Antibody (SR2049) [NBP3-21568] - Western blot analysis using NBP3-21568 in (1) MCF-7 cell treated with Alkaline Phosphatase lysate; (2) MCF-7 cell lysate.")
![Western Blot: AKT1 [p Thr450] Antibody (S07-4A7) [NBP3-19824] - Western blot detection of phospho-AKT1 (Thr450) in U2OS, C6 cell lysates using NBP3-19824 (1:1000 diluted). Predicted band size: 56kDa. Observed band size: 56kDa.](http://images.novusbio.com/fullsize/AKT1-p-Thr450-Antibody-S07-4A7-Western-Blot-NBP3-19824-img0001.jpg "Western Blot: AKT1 [p Thr450] Antibody (S07-4A7) [NBP3-19824] - Western blot detection of phospho-AKT1 (Thr450) in U2OS, C6 cell lysates using NBP3-19824 (1:1000 diluted). Predicted band size: 56kDa. Observed band size: 56kDa.")
![Western Blot: AKT [p Ser473] Antibody (PSH04-44) [NBP3-32747] - Western blot analysis of AKT on different lysates with Rabbit anti-AKT antibody (NBP3-32747) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HEK-293 treated with 50uM LY294002 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 56 kDa Observed band size: 56 kDa Exposure time: 20 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32747) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32747_rabbit-akt-p-ser473-mab-psh04-44-278202415242614.jpg "Western Blot: AKT [p Ser473] Antibody (PSH04-44) [NBP3-32747] - Western blot analysis of AKT on different lysates with Rabbit anti-AKT antibody (NBP3-32747) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HEK-293 treated with 50uM LY294002 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 56 kDa Observed band size: 56 kDa Exposure time: 20 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32747) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: AKT1 [p Ser129] Antibody (JE59-50) [NBP3-32748] - Western blot analysis of AKT1 on different lysates with Rabbit anti-AKT1 antibody (NBP3-32748) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: MCF7 treated pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 56 kDa Observed band size: 60 kDa Exposure time: 3 minutes 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32748) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32748_rabbit-akt1-p-ser129-mab-je59-50-27820241631645.jpg "Western Blot: AKT1 [p Ser129] Antibody (JE59-50) [NBP3-32748] - Western blot analysis of AKT1 on different lysates with Rabbit anti-AKT1 antibody (NBP3-32748) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: MCF7 treated pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 56 kDa Observed band size: 60 kDa Exposure time: 3 minutes 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32748) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: AKT1 Antibody (CL0899) [NBP2-52957] - WB using AKT1 antibody. Lane 1: Marker [kDa], Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/AKT1-Antibody-CL0899-Western-Blot-NBP2-52957-img0001.jpg "Western Blot: AKT1 Antibody (CL0899) [NBP2-52957] - WB using AKT1 antibody. Lane 1: Marker [kDa], Lane 2: Human cell line RT-4")
![Western Blot: AKT [p Thr308] Antibody (2O2W6) [NBP3-16956] - Western blot analysis of extracts of various cell lines, using Phospho-Akt-T308 antibody (NBP3-16956) at 1:1000 dilution.NIH/3T3 and C6 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/AKT-p-Thr308-Antibody-2O2W6-Western-Blot-NBP3-16956-img0001.jpg "Western Blot: AKT [p Thr308] Antibody (2O2W6) [NBP3-16956] - Western blot analysis of extracts of various cell lines, using Phospho-Akt-T308 antibody (NBP3-16956) at 1:1000 dilution.NIH/3T3 and C6 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")