| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21578-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22614-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26514-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-27692-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44500 | |||
| BSA Free | NBP2-52956-25ul | |||
![Western Blot: AKT [p Thr450] Antibody [NBP3-13168] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT (phospho Thr450) antibody (NBP3-13168) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/AKT-[p-Thr450]-Antibody-Western-Blot-NBP3-13168-img0004.jpg "Western Blot: AKT [p Thr450] Antibody [NBP3-13168] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT (phospho Thr450) antibody (NBP3-13168) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: AKT [p Thr450] Antibody [NBP3-13168] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT (phospho Thr450) antibody (NBP3-13168) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/AKT-[p-Thr450]-Antibody-Western-Blot-NBP3-13168-img0002.jpg)
![Western Blot: AKT [p Thr450] Antibody [NBP3-13168] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT (phospho Thr450) antibody (NBP3-13168) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/AKT-[p-Thr450]-Antibody-Western-Blot-NBP3-13168-img0003.jpg)
![Immunohistochemistry: AKT [p Ser 473] Antibody (SR2061) [NBP3-21578] - Immunohistochemical analysis of paraffin-embedded human kidney, using NBP3-21578.](http://images.novusbio.com/fullsize/nbp3-21578_rabbit-akt-p-ser-473-mab-sr2061-57202315545125.jpg "Immunohistochemistry: AKT [p Ser 473] Antibody (SR2061) [NBP3-21578] - Immunohistochemical analysis of paraffin-embedded human kidney, using NBP3-21578.")
![Western Blot: AKT [p Ser 473] Antibody (SR2061) [NBP3-21578] - Western blot analysis on 2 NIH/3T3 cell lysate treated with PDGF using NBP3-21578.](http://images.novusbio.com/fullsize/nbp3-21578_rabbit-akt-p-ser-473-mab-sr2061-6720239533735.jpg)
![Immunocytochemistry/Immunofluorescence: AKT [p Ser 473] Antibody (SR2061) [NBP3-21578] - Immunofluorescent analysis of NIH/3T3 cells treated with PDGF, using NBP3-21578.](http://images.novusbio.com/fullsize/nbp3-21578_rabbit-akt-p-ser-473-mab-sr2061-6720231051266.jpg)
![Western Blot: AKT1 Antibody (PSH0-50) [NBP3-32016] - Western blot analysis of AKT1 on different lysates with Rabbit anti-AKT1 antibody (NBP3-32016) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: MCF7 cell lysate Lane 3: HeLa cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: C2C12 cell lysate Lane 6: COS-1 cell lysate Lane 7: PC-12 cell lysate Lane 8: Mouse brain tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 56 kDa Observed band size: 60 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32016) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32016_rabbit-akt1-mab-psh0-50-27820241514178.jpg "Western Blot: AKT1 Antibody (PSH0-50) [NBP3-32016] - Western blot analysis of AKT1 on different lysates with Rabbit anti-AKT1 antibody (NBP3-32016) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: MCF7 cell lysate Lane 3: HeLa cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: C2C12 cell lysate Lane 6: COS-1 cell lysate Lane 7: PC-12 cell lysate Lane 8: Mouse brain tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 56 kDa Observed band size: 60 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32016) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: AKT1 Antibody (PSH0-50) [NBP3-32016] - Immunocytochemistry analysis of Jurkat cells labeling AKT1 with Rabbit anti-AKT1 antibody (NBP3-32016) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-AKT1 antibody (NBP3-32016) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32016_rabbit-akt1-mab-psh0-50-2920241192386.jpg)
![Flow Cytometry: AKT1 Antibody (PSH0-50) [NBP3-32016] - Flow cytometric analysis of Jurkat cells labeling AKT1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32016, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32016_rabbit-akt1-mab-psh0-50-39202488468.jpg)
![Western Blot: AKT1/2/3 Antibody (SR2003) [NBP3-22614] - Western blot analysis using NBP3-22614 in (1) Hela cell lysate;(2) A549 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22614_rabbit-akt1-2-3-mab-sr2003-57202317215156.jpg "Western Blot: AKT1/2/3 Antibody (SR2003) [NBP3-22614] - Western blot analysis using NBP3-22614 in (1) Hela cell lysate;(2) A549 cell lysate.")
![Immunocytochemistry/ Immunofluorescence: AKT1/2/3 Antibody (JE75-09) [NBP3-32017] - Immunocytochemistry analysis of MCF7 cells labeling AKT1/2/3 with Rabbit anti-AKT1/2/3 antibody (NBP3-32017) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-AKT1/2/3 antibody (NBP3-32017) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32017_rabbit-akt1-2-3-mab-je75-09-29202410334024.jpg "Immunocytochemistry/ Immunofluorescence: AKT1/2/3 Antibody (JE75-09) [NBP3-32017] - Immunocytochemistry analysis of MCF7 cells labeling AKT1/2/3 with Rabbit anti-AKT1/2/3 antibody (NBP3-32017) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-AKT1/2/3 antibody (NBP3-32017) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.")
![Flow Cytometry: AKT1/2/3 Antibody (JE75-09) [NBP3-32017] - Flow cytometric analysis of MCF7 cells labeling AKT1/2/3. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32017, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32017_rabbit-akt1-2-3-mab-je75-09-29202416453243.jpg)
![Western Blot: AKT1/2/3 Antibody (JE75-09) [NBP3-32017] - Western blot analysis of AKT1/2/3 on different lysates with Rabbit anti-AKT1/2/3 antibody (NBP3-32017) at 1/2,000 dilution.Lane 1: HeLa cell lysateLane 2: MCF7 cell lysateLane 3: A549 cell lysateLane 4: U-2 OS cell lysateLane 5: NIH/3T3 cell lysateLysates/proteins at 15 µg/Lane.Predicted band size: 56 kDaObserved band size: 56 kDaExposure time: 30 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32017_rabbit-akt1-2-3-mab-je75-09-3920248453538.jpg)
![Western Blot: AKT [p Ser473] Antibody [NBP3-13120] - Untreated (-) and treated (+) MCF-7 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT (phospho Ser473) antibody (NBP3-13120) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/AKT-[p-Ser473]-Antibody-Western-Blot-NBP3-13120-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: AKT [p Ser473] Antibody [NBP3-13120] - Untreated (-) and treated (+) MCF-7 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT (phospho Ser473) antibody (NBP3-13120) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Western Blot: AKT [p Ser473] Antibody [NBP3-13120] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT (phospho Ser473) antibody (NBP3-13120) diluted at 1:1000.](http://images.novusbio.com/fullsize/AKT-[p-Ser473]-Antibody-Western-Blot-NBP3-13120-img0001.jpg)
![Western Blot: AKT [p Ser473] Antibody [NBP3-13120] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT (phospho Ser473) antibody (NBP3-13120) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/AKT-[p-Ser473]-Antibody-Western-Blot-NBP3-13120-img0002.jpg)
![Immunohistochemistry: AKT1/2 Antibody (1G0K5) [NBP3-33526] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using AKT1/2 Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-33526_rabbit-akt1-2-mab-1g0k5-91220241519579.jpg "Immunohistochemistry: AKT1/2 Antibody (1G0K5) [NBP3-33526] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using AKT1/2 Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Western Blot: AKT1/2 Antibody (1G0K5) [NBP3-33526] - Western blot analysis of various lysates using AKT1/2 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-33526_rabbit-akt1-2-mab-1g0k5-912202415952.jpg)
![Immunohistochemistry: AKT1/2/3 Antibody (16E10) [NBP3-26514] - Image of AKT1/2/3 Antibody (16E10) diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.54% DAB.](http://images.novusbio.com/fullsize/nbp3-26514_rabbit-akt1-2-3-mab-16e10-2722024847194.jpg "Immunohistochemistry: AKT1/2/3 Antibody (16E10) [NBP3-26514] - Image of AKT1/2/3 Antibody (16E10) diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.54% DAB.")
![Western Blot: AKT1/2/3 Antibody (16E10) [NBP3-26514] - Positive Western Blot detected in: SH-SY5Y whole cell lysate. All lanes: AKT1/2/3 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 56 kDa Observed band size: 56 kDa](http://images.novusbio.com/fullsize/nbp3-26514_rabbit-akt1-2-3-mab-16e10-282202411442636.jpg)
![Flow Cytometry: AKT1/2/3 Antibody (16E10) [NBP3-26514] - Overlay Peak curve showing Jurkat cells stained with AKT1/2/3 Antibody (16E10) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26514_rabbit-akt1-2-3-mab-16e10-28220241418367.jpg)
![Western Blot: AKT1/2 Antibody (SR2005) [NBP3-27692] - Western blot analysis of AKT1/2 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate.](http://images.novusbio.com/fullsize/nbp3-27692_rabbit-akt1-2-mab-sr2005-75202410133496.jpg "Western Blot: AKT1/2 Antibody (SR2005) [NBP3-27692] - Western blot analysis of AKT1/2 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate.")
![Western Blot: AKT1 Antibody (CL0887) [NBP2-52956] - WB using AKT1 antibody. Lane 1: Marker [kDa], Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/AKT1-Antibody-CL0887-Western-Blot-NBP2-52956-img0001.jpg "Western Blot: AKT1 Antibody (CL0887) [NBP2-52956] - WB using AKT1 antibody. Lane 1: Marker [kDa], Lane 2: Human cell line RT-4")
![Immunocytochemistry/Immunofluorescence: AKT1 Antibody (CL0887) [NBP2-52956] - Staining of MCF7 cells using the Anti-AKT1 monoclonal antibody, showing specific staining in the nucleus, cytosol and plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/AKT1-Antibody-CL0887-Immunocytochemistry-Immunofluorescence-NBP2-52956-img0002.jpg)
![Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody (RM251) [NBP2-61475] - IHC-P staining of FFPE human breast cancer tissue sections using NBP2-61475 at a 1:400 dilution.](http://images.novusbio.com/fullsize/nbp2-61475_rabbit-akt1-p-ser473-mab-rm251-immunohistochemistry-paraffin-189202523512118.png "Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody (RM251) [NBP2-61475] - IHC-P staining of FFPE human breast cancer tissue sections using NBP2-61475 at a 1:400 dilution.")
![Western Blot: AKT1 [p Ser473] Antibody (RM251) [NBP2-61475] - WB of lysates from 293 cells either grown in medium with serum, serum starved, or insulin treated using NBP2-61475 at 1:1000 dilution.](http://images.novusbio.com/fullsize/nbp2-61475_rabbit-akt1-p-ser473-mab-rm251-western-blot-18920252352110.png)
![SDS-Page: Recombinant Human AKT1 Protein [NBP2-52121] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain](http://images.novusbio.com/fullsize/Recombinant-Human-AKT1-Protein-SDS-Page-NBP2-52121-img0001.jpg "SDS-Page: Recombinant Human AKT1 Protein [NBP2-52121] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain")