| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70150 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92604-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-99337-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70150 | |||
![Western Blot: ABCD1 Antibody (OTI2C12) [NBP2-46476] - Western blot shows lysates of TIME human endothelial parental cell line and ABCD1 knockout (KO) TIME cell line. PVDF membrane was probed with 1:2000 of Mouse Anti-Human ABCD1 Monoclonal Antibody (Catalog # NBP2-46476) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for ABCD1 at approximately 75 kDa (as indicated) in the parental TIME cell line, but is not detectable in the knockout TIME cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/ABCD1-Antibody-OTI2C12-Knockdown-Validated-NBP2-46476-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ABCD1 Antibody (OTI2C12) [NBP2-46476] - Western blot shows lysates of TIME human endothelial parental cell line and ABCD1 knockout (KO) TIME cell line. PVDF membrane was probed with 1:2000 of Mouse Anti-Human ABCD1 Monoclonal Antibody (Catalog # NBP2-46476) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for ABCD1 at approximately 75 kDa (as indicated) in the parental TIME cell line, but is not detectable in the knockout TIME cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: ABCD1 Antibody (OTI2C12) [NBP2-46476] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ABCD1.](http://images.novusbio.com/fullsize/ABCD1-Antibody-OTI2C12-Western-Blot-NBP2-46476-img0004.jpg)
![Immunohistochemistry: ABCD1 Antibody (OTI2C12) [NBP2-46476] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ABCD1-Antibody-OTI2C12-Immunohistochemistry-NBP2-46476-img0003.jpg)
![Immunohistochemistry: ABCD1 Antibody (OTI2C12) [NBP2-46476] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ABCD1-Antibody-OTI2C12-Immunohistochemistry-NBP2-46476-img0001.jpg)
![Immunohistochemistry: ABCD1 Antibody (OTI2C12) [NBP2-46476] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ABCD1-Antibody-OTI2C12-Immunohistochemistry-NBP2-46476-img0002.jpg)
![Western Blot: ABCD1 Antibody (OTI2C12) - Azide and BSA Free [NBP2-70150] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ABCD1.](http://images.novusbio.com/fullsize/ABCD1-Antibody-OTI2C12-Azide-and-BSA-Free-Western-Blot-NBP2-70150-img0001.jpg "Western Blot: ABCD1 Antibody (OTI2C12) - Azide and BSA Free [NBP2-70150] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ABCD1.")
![Immunohistochemistry: ABCD1 Antibody (OTI2C12) - Azide and BSA Free [NBP2-70150] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ABCD1-Antibody-OTI2C12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70150-img0004.jpg)
![Immunohistochemistry: ABCD1 Antibody (OTI2C12) - Azide and BSA Free [NBP2-70150] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ABCD1-Antibody-OTI2C12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70150-img0002.jpg)
![Immunohistochemistry: ABCD1 Antibody (OTI2C12) - Azide and BSA Free [NBP2-70150] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ABCD1-Antibody-OTI2C12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70150-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: ABCD1 Antibody (OTI2C12) - Azide and BSA Free [NBP2-70150] - Western blot shows lysates of TIME human endothelial parental cell line and ABCD1 knockout (KO) TIME cell line. PVDF membrane was probed with 1:2000 of Mouse Anti-Human ABCD1 Monoclonal Antibody (Catalog # NBP2-46476) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for ABCD1 at approximately 75 kDa (as indicated) in the parental TIME cell line, but is not detectable in the knockout TIME cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/ABCD1-Antibody-OTI2C12-Azide-and-BSA-Free-Knockout-Validated-NBP2-70150-img0005.jpg)
![Western Blot: ABCD1 Antibody [NBP2-92604] - Analysis of extracts of various cell lines, using ABCD1 at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/ABCD1-Antibody-Western-Blot-NBP2-92604-img0002.jpg "Western Blot: ABCD1 Antibody [NBP2-92604] - Analysis of extracts of various cell lines, using ABCD1 at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.")
![Immunocytochemistry/ Immunofluorescence: ABCD1 Antibody - Azide and BSA Free [NBP2-92604] - Immunofluorescence analysis of NIH/3T3 cells using ABCD1 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92604_rabbit-polyclonal-abcd1-antibody-42202514264440.jpg%3Ft=1709092081)
![Western Blot: ABCD1 Antibody (9R4O8) [NBP3-33443] - Western blot analysis of lysates from NIH/3T3 cells, using ABCD1 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-33443_rabbit-abcd1-mab-9r4o8-1012202411364656.jpg "Western Blot: ABCD1 Antibody (9R4O8) [NBP3-33443] - Western blot analysis of lysates from NIH/3T3 cells, using ABCD1 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.")
![Western Blot: ABCD1 Antibody (9R4O8) [NBP3-33443] - Western blot analysis of various lysates, using ABCD1 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.](http://images.novusbio.com/fullsize/nbp3-33443_rabbit-abcd1-mab-9r4o8-1012202411404082.jpg)
![Immunocytochemistry/ Immunofluorescence: ABCD1 Antibody (9D11) [NBP3-42921] - Immunofluorescence analysis of Hela cells using (ABCD1 antibody) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-42921_mouse-abcd1-mab-9d11-10122024173412.jpg "Immunocytochemistry/ Immunofluorescence: ABCD1 Antibody (9D11) [NBP3-42921] - Immunofluorescence analysis of Hela cells using (ABCD1 antibody) at dilution of 1:100. Blue: DAPI for nuclear staining.")
![Western Blot: ABCD1 Antibody (9D11) [NBP3-42921] - HeLa cells were subjected to SDS PAGE followed by western blot with (ABCD1 antibody) at dilution of 1:400](http://images.novusbio.com/fullsize/nbp3-42921_mouse-abcd1-mab-9d11-101220241715426.jpg)
![Western Blot: ABCD1 Antibody [NB100-96913] - HEK293 overexpressing human ABCD1 and probed with NB100-96913 at 1ug/ml (mock transfection in first lane).](http://images.novusbio.com/fullsize/ABCD1-Antibody-Western-Blot-NB100-96913-img0002.jpg "Western Blot: ABCD1 Antibody [NB100-96913] - HEK293 overexpressing human ABCD1 and probed with NB100-96913 at 1ug/ml (mock transfection in first lane).")
![Immunocytochemistry/Immunofluorescence: ABCD1 Antibody [NBP2-99337] - Immunofluorescence staining of ABCD1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human ABCD1 polyclonal antibody (dilution ratio 1:500) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/ABCD1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-99337-img0001.jpg "Immunocytochemistry/Immunofluorescence: ABCD1 Antibody [NBP2-99337] - Immunofluorescence staining of ABCD1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human ABCD1 polyclonal antibody (dilution ratio 1:500) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.")
![Immunohistochemistry-Paraffin: ABCD1 Antibody [NBP2-99337] - Immunochemical staining of human ABCD1 in human pancreas with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/ABCD1-Antibody-Immunohistochemistry-Paraffin-NBP2-99337-img0002.jpg)
![SDS-Page: Recombinant Human ABCD1 Protein [H00000215-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-ABCD1-Protein-SDS-Page-H00000215-P01-img0001.jpg "SDS-Page: Recombinant Human ABCD1 Protein [H00000215-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: ABCD1 Overexpression Lysate (Adult Normal) [NBL1-07179] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ABCD1.](http://images.novusbio.com/fullsize/ABCD1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07179-img0002.jpg "Western Blot: ABCD1 Overexpression Lysate (Adult Normal) [NBL1-07179] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ABCD1.")