HAF016). A specific band was detected for MELK at approximately 74 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of Huh-7 human hepatoma cell line, MO7e human megakaryocytic leukemic cell line, and THP-1 human acute monocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human MELK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4820) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MELK at approximately 74 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF4820-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37481-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83031-25ul | |||
CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of glandular epithelial cells. View our protocol for 
![Immunocytochemistry/Immunofluorescence: MELK Antibody (2G2) [NBP2-37481] - Immunofluorescence analysis of HepG2 cells using MELK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/MELK-Antibody-2G2-Immunocytochemistry-Immunofluorescence-NBP2-37481-img0002.jpg "Immunocytochemistry/Immunofluorescence: MELK Antibody (2G2) [NBP2-37481] - Immunofluorescence analysis of HepG2 cells using MELK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.")
![Immunohistochemistry: MELK Antibody (2G2) [NBP2-37481] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using MELK mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/MELK-Antibody-2G2-Immunohistochemistry-NBP2-37481-img0005.jpg)
![Flow Cytometry: MELK Antibody (2G2) [NBP2-37481] - Flow cytometric analysis of MCF-7 cells using MELK mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/MELK-Antibody-2G2-Flow-Cytometry-NBP2-37481-img0003.jpg)
![Immunohistochemistry: MELK Antibody (2G2) [NBP2-37481] - Immunohistochemical analysis of paraffin-embedded liver cancer tissues using MELK mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/MELK-Antibody-2G2-Immunohistochemistry-NBP2-37481-img0004.jpg)
![ELISA: MELK Antibody (2G2) [NBP2-37481] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/MELK-Antibody-2G2-ELISA-NBP2-37481-img0001.jpg)
![Western Blot: MELK Antibody [NB100-782] - MELK in human testes lysate (RIPA buffer, 1.4E5 cells per lane) using this antibody at 0.1 ug/ml.](http://images.novusbio.com/fullsize/MELK-Antibody-Western-Blot-NB100-782-img0006.jpg "Western Blot: MELK Antibody [NB100-782] - MELK in human testes lysate (RIPA buffer, 1.4E5 cells per lane) using this antibody at 0.1 ug/ml.")
![Western Blot: MELK Antibody [NB100-782] - NB100-782 (0.1ug/ml) staining of HEPG2 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Online Catalogue NAC1 to Quaking > Goat Anti-PPP1R15A Antibody](http://images.novusbio.com/fullsize/MELK-Antibody-Western-Blot-NB100-782-img0001.jpg)
![Western Blot: MELK Antibody [NB100-782] - NB 100-782 (0.5ug/ml) staining of Jurkat lysate (RIPA buffer, 1.4E5 cells per lane). Detected by western blot using chemiluminescence.](http://images.novusbio.com/fullsize/MELK-Antibody-Western-Blot-NB100-782-img0004.jpg)
![Western Blot: MELK Antibody [NBP1-83031] - Analysis in control (vector only transfected HEK293T lysate) and MELK over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/MELK-Antibody-Western-Blot-NBP1-83031-img0003.jpg "Western Blot: MELK Antibody [NBP1-83031] - Analysis in control (vector only transfected HEK293T lysate) and MELK over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: MELK Antibody [NBP1-83031] - Staining of human stomach shows strong cytoplasmic and nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/MELK-Antibody-Immunohistochemistry-Paraffin-NBP1-83031-img0002.jpg)
![Immunohistochemistry: MELK Antibody (PSH03-55) [NBP3-32587] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-MELK antibody (NBP3-32587) at 1/500 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32587) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32587_rabbit-melk-mab-psh03-55-298202413585532.jpg "Immunohistochemistry: MELK Antibody (PSH03-55) [NBP3-32587] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-MELK antibody (NBP3-32587) at 1/500 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32587) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western blot analysis on different lysates with NBP3-32587 at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: MDA-MB-231 cell lysate Lane 4: HCT 116 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C2C12 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 75 kDa Observed band size: 72 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32587 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32587_-western-blot-639204597148369214.jpg)
![Western Blot: MELK Antibody (4F10X4) [NBP3-16211] - Western blot analysis of extracts of various cell lines, using MELK Rabbit mAb (NBP3-16211) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](http://images.novusbio.com/fullsize/MELK-Antibody-4F10X4-Western-Blot-NBP3-16211-img0001.jpg "Western Blot: MELK Antibody (4F10X4) [NBP3-16211] - Western blot analysis of extracts of various cell lines, using MELK Rabbit mAb (NBP3-16211) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
![SDS-Page: Recombinant Human MELK Protein [H00009833-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00009833-Q01-1.jpg "SDS-Page: Recombinant Human MELK Protein [H00009833-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: MELK Overexpression Lysate (Adult Normal) [NBL1-13007] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MELK.](http://images.novusbio.com/fullsize/MELK-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13007-img0002.jpg "Western Blot: MELK Overexpression Lysate (Adult Normal) [NBL1-13007] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MELK.")
![Western Blot: MELK Antibody (S06-4D6) [NBP3-15059] - Western blot detection of MELK in U2OS, C6 cell lysates using MELK Rabbit mAb (1:1000 diluted). Predicted band size: 75kDa. Observed band size: 75kDa.](http://images.novusbio.com/fullsize/MELK-Antibody-S06-4D6-Western-Blot-NBP3-15059-img0001.jpg "Western Blot: MELK Antibody (S06-4D6) [NBP3-15059] - Western blot detection of MELK in U2OS, C6 cell lysates using MELK Rabbit mAb (1:1000 diluted). Predicted band size: 75kDa. Observed band size: 75kDa.")
![ELISA: Human MELK - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39806] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39806_human-melk-ready-to-use-elisa-kit-colorimetric-2110202415371318.png "ELISA: Human MELK - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39806] - Standard Curve Reference")
![ELISA: Human MELK ELISA Kit (Colorimetric) [NBP3-39805] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39805_human-melk-elisa-kit-colorimetric-2110202415264322.png "ELISA: Human MELK ELISA Kit (Colorimetric) [NBP3-39805] - Standard Curve Reference")
