| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26673-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22085-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99507-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99764-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89876-50ul | |||
![Immunohistochemistry: CD97 Antibody (3A7) [NBP3-26673] - Image of CD97 Antibody (3A7) diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26673_rabbit-cd97-mab-3a7-262202414471599.jpg "Immunohistochemistry: CD97 Antibody (3A7) [NBP3-26673] - Image of CD97 Antibody (3A7) diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Immunohistochemistry: CD97 Antibody (3A7) [NBP3-26673] - Image of CD97 Antibody (3A7) diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26673_rabbit-cd97-mab-3a7-262202415475633.jpg)
![Immunocytochemistry/Immunofluorescence: CD97 Antibody (3A7) [NBP3-26673] - Staining of Hela cells with CD97 Antibody (3A7) at 1:87.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26673_rabbit-cd97-mab-3a7-282202412475124.jpg)
![Western Blot: CD97 Antibody (SR1972) [NBP3-22085] - Western blot analysis using NBP3-22085 in human tonsil cell lysate.](http://images.novusbio.com/fullsize/nbp3-22085_rabbit-cd97-mab-sr1972-572023159552.jpg "Western Blot: CD97 Antibody (SR1972) [NBP3-22085] - Western blot analysis using NBP3-22085 in human tonsil cell lysate.")
![Flow Cytometry: CD97 Antibody (MEM-180) [NB500-425] - Using the FITC direct conjugate Surface staining of human peripheral blood with anti-CD97 (MEM-180) FITC.](http://images.novusbio.com/fullsize/CD97-Antibody-MEM-180-Flow-Cytometry-NB500-425-img0002.jpg "Flow Cytometry: CD97 Antibody (MEM-180) [NB500-425] - Using the FITC direct conjugate Surface staining of human peripheral blood with anti-CD97 (MEM-180) FITC.")
![Flow Cytometry: CD97 Antibody (MEM-180) [NB500-425] - Surface staining of human peripheral blood with anti-CD97 (MEM-180) purified, GAM-APC.](http://images.novusbio.com/fullsize/CD97-Antibody-MEM-180-Flow-Cytometry-NB500-425-img0001.jpg)
![Western Blot: CD97 Antibody [NBP2-99507] - Anti-CD97 rabbit polyclonal antibody at 1:500 dilution. Lane A: NIH/3T3 Whole Cell Lysate. Lane B: HepG2 Whole Cell Lysate. Lane C: Raw264.7 Whole Cell Lysate. Lane D: Mouse spleen tissue lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 92 kDa](http://images.novusbio.com/fullsize/CD97-Antibody-Western-Blot-NBP2-99507-img0002.jpg "Western Blot: CD97 Antibody [NBP2-99507] - Anti-CD97 rabbit polyclonal antibody at 1:500 dilution. Lane A: NIH/3T3 Whole Cell Lysate. Lane B: HepG2 Whole Cell Lysate. Lane C: Raw264.7 Whole Cell Lysate. Lane D: Mouse spleen tissue lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 92 kDa")
![Immunoprecipitation: CD97 Antibody [NBP2-99507] - CD97 was immunoprecipitated using: Lane A: 0.5 mg NIH/3T3 Whole Cell Lysate. 4 ul anti-CD97 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-CD97 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 92 kDa. Observed band size: 70 kDa](http://images.novusbio.com/fullsize/CD97-Antibody-Immunoprecipitation-NBP2-99507-img0003.jpg)
![Western Blot: CD97 Antibody [NBP2-99764] - Anti-CD97 rabbit polyclonal antibody at 1:500 dilution. Lane A: HL-60 Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 92 kDa. Observed band size: 92 kDa](http://images.novusbio.com/fullsize/CD97-Antibody-Western-Blot-NBP2-99764-img0004.jpg "Western Blot: CD97 Antibody [NBP2-99764] - Anti-CD97 rabbit polyclonal antibody at 1:500 dilution. Lane A: HL-60 Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 92 kDa. Observed band size: 92 kDa")
![Immunoprecipitation: CD97 Antibody [NBP2-99764] - CD97 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg SW480 Whole Cell Lysate0.5 ul anti-CD97 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-CD97 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 92 kDa. Observed band size: 92 kDa](http://images.novusbio.com/fullsize/CD97-Antibody-Immunoprecipitation-NBP2-99764-img0003.jpg)
![Flow Cytometry: CD97 Antibody (024) [NBP2-89876] - Analysis of Human CD97 expression on K562 cells. Cells were stained with purified anti-Human CD97, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/CD97-Antibody-024-Flow-Cytometry-NBP2-89876-img0002.jpg "Flow Cytometry: CD97 Antibody (024) [NBP2-89876] - Analysis of Human CD97 expression on K562 cells. Cells were stained with purified anti-Human CD97, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![Immunocytochemistry/Immunofluorescence: CD97 Antibody (024) [NBP2-89876] - Staining of Human CD97 in JURKAT cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-89876 antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cells membranes.](http://images.novusbio.com/fullsize/nbp2-89876_rabbit-monoclonal-cd97-antibody-024-23520252135158.png)
![ELISA: Rat CD97 ELISA Kit (Colorimetric) [NBP2-75165] - Samples were spiked with high concentrations of Rat CD97 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75165_rat-cd97-elisa-kit-colorimetric-132202416133678.jpg "ELISA: Rat CD97 ELISA Kit (Colorimetric) [NBP2-75165] - Samples were spiked with high concentrations of Rat CD97 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat CD97 ELISA Kit (Colorimetric) [NBP2-75165] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75165_rat-cd97-elisa-kit-colorimetric-432024903599.jpg)
![ELISA: Mouse CD97 ELISA Kit (Colorimetric) [NBP2-75164] - Samples were spiked with high concentrations of Mouse CD97 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75164_mouse-cd97-elisa-kit-colorimetric-13220241613024.jpg "ELISA: Mouse CD97 ELISA Kit (Colorimetric) [NBP2-75164] - Samples were spiked with high concentrations of Mouse CD97 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse CD97 ELISA Kit (Colorimetric) [NBP2-75164] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75164_mouse-cd97-elisa-kit-colorimetric-4320249192869.jpg)
![Western Blot: CD97 Antibody (3E5L8) [NBP3-33340] - Western blot analysis of lysates from U-937 cells, using CD97 Rabbit mAb at1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:200000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-33340_rabbit-cd97-mab-3e5l8-912202414112840.jpg "Western Blot: CD97 Antibody (3E5L8) [NBP3-33340] - Western blot analysis of lysates from U-937 cells, using CD97 Rabbit mAb at1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:200000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.")
![Western Blot: CD97 Overexpression Lysate (Adult Normal) [NBL1-08984] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CD97.](http://images.novusbio.com/fullsize/CD97-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-08984-img0002.jpg "Western Blot: CD97 Overexpression Lysate (Adult Normal) [NBL1-08984] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CD97.")