Products Related to Gene-ID: 943

NL001) and counterstained with DAPI (blue). Specific staining was localized to cell membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cell membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="CD30/TNFRSF8 was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Goat Anti-Human CD30/TNFRSF8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF229) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cell membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

MAB2291) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human CD30/TNFRSF8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF229) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_lightbox"> MAB2291) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human CD30/TNFRSF8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF229) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_thumb" />

MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." alt="Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human CD30/TNFRSF8 Monoclonal Antibody (Catalog # MAB229, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

D6050). The ED50 for this effect is typically 0.05- 0.2 µg/mL." class="big_lightbox"> D6050). The ED50 for this effect is typically 0.05- 0.2 µg/mL." class="big_thumb" />

Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> Fluorescent ICC Staining of Non-adherent Cells." alt="CD30/TNFRSF8 was detected in immersion fixed mouse splenocytes using 15 µg/mL Goat Anti-Mouse CD30/TNFRSF8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF852) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

852-CD) was serially diluted and captured by Rat Anti-Mouse CD30/TNFRSF8 Monoclonal Antibody (Catalog # MAB8521) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CD30/TNFRSF8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF852) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 852-CD) was serially diluted and captured by Rat Anti-Mouse CD30/TNFRSF8 Monoclonal Antibody (Catalog # MAB8521) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CD30/TNFRSF8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF852) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane and cytoplasm. Protocol available in COMET™ Panel Builder.​" class="big_lightbox"> DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane and cytoplasm. Protocol available in COMET™ Panel Builder.​" alt="CD30/TNFRSF8 was detected in immersion fixed paraffin-embedded sections of human Hodgkin’s Lymphoma using Mouse Anti-Human CD30/TNFRSF8, Monoclonal Antibody (Catalog# MAB11768) at 25ug/mL at 37° Celsius for 16 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane and cytoplasm. Protocol available in COMET™ Panel Builder.​" class="big_thumb" />

HAF018). Specific bands were detected for CD30/TNFRSF8 at approximately 90kDa and 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF018). Specific bands were detected for CD30/TNFRSF8 at approximately 90kDa and 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to the cell membrane of Reed Sternberg cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to the cell membrane of Reed Sternberg cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

852-CD) was serially diluted and captured by Rat Anti-Mouse CD30/TNFRSF8 Monoclonal Antibody (Catalog # MAB8521) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CD30/TNFRSF8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF852) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 852-CD) was serially diluted and captured by Rat Anti-Mouse CD30/TNFRSF8 Monoclonal Antibody (Catalog # MAB8521) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CD30/TNFRSF8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF852) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Mouse CD30/TNFRSF8 Fc Chimera (Catalog # 852-CD) was serially diluted and captured by Rat Anti-Mouse CD30/TNFRSF8 Monoclonal Antibody (Catalog # MAB8521) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CD30/TNFRSF8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF852) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

F0135). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0135). View our protocol for Staining Membrane-associated Proteins." alt="Jurkat human acute T cell leukemia cell line was stained with Human Anti-Human CD30/TNFRSF8 (Brentuximab Biosimilar) Monoclonal Antibody (Catalog # MAB9576, filled histogram) or irrelevant antibody (open histogram) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

DY990). Goat Anti-Human CD30/TNFRSF8 Antigen Affinity-purified Polyclonal Antibody (AF229) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_lightbox"> DY990). Goat Anti-Human CD30/TNFRSF8 Antigen Affinity-purified Polyclonal Antibody (AF229) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." alt="Recombinant Human CD30/TNFRSF8 protein was serially diluted 2-fold and captured by Mouse Anti-Human CD30/TNFRSF8 Monoclonal Antibody (Catalog # MAB2291) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human CD30/TNFRSF8 Antigen Affinity-purified Polyclonal Antibody (AF229) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_thumb" />

1028-CL) with an ED₅₀ of 0.800‑16.0 ng/mL." class="big_lightbox"> 1028-CL) with an ED₅₀ of 0.800‑16.0 ng/mL." alt="In a functional ELISA, Biotinylated Recombinant Human CD30/TNFRSF8 Fc Chimera Protein (Catalog # BT11155) binds to Recombinant Human CD30 Ligand/TNFSF8 (1028-CL) with an ED₅₀ of 0.800‑16.0 ng/mL." class="big_thumb" />

732-CL) is immobilized at 0.250 μg/mL (100 μL/well), Recombinant Rat CD30/TNFRSF8 Mouse Fc Chimera Protein (Catalog # 11034-CD) binds with and ED₅₀ of 20.0-120 ng/mL." class="big_lightbox"> 732-CL) is immobilized at 0.250 μg/mL (100 μL/well), Recombinant Rat CD30/TNFRSF8 Mouse Fc Chimera Protein (Catalog # 11034-CD) binds with and ED₅₀ of 20.0-120 ng/mL." alt="When Recombinant Mouse CD30 Ligand/TNFSF8 (732-CL) is immobilized at 0.250 μg/mL (100 μL/well), Recombinant Rat CD30/TNFRSF8 Mouse Fc Chimera Protein (Catalog # 11034-CD) binds with and ED₅₀ of 20.0-120 ng/mL." class="big_thumb" />