Products Related to Gene-ID: 920

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="CD4 was detected in immersion fixed feline peripheral blood mononuclear cells (PBMCs) using Goat Anti-Feline CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2597) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B)." class="big_lightbox"> MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B)." alt="Cotton rat splenocytes were stained with Mouse Anti-Cotton Rat CD4 Monoclonal Antibody (Catalog # MAB6676, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B)." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="CD4 was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Canine CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2410) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

HAF018). A specific band was detected for CD4 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for CD4 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of rat spleen tissue and rat CD4⁺T Cells. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Rat CD4 Monoclonal Antibody (Catalog # MAB6439) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CD4 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF018). A specific band was detected for CD4 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for CD4 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> Fluorescent ICC Staining of Non-adherent Cells." alt="CD4 was detected in immersion fixed non-stimulated feline peripheral blood mononuclear cells (PBMCs) using 25 µg/mL Mouse Anti-Feline CD4 Monoclonal Antibody (Catalog # MAB25971) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127)." class="big_lightbox"> 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127)." alt="Cotton rat splenocytes were stained with Sheep Anti-Cotton Rat CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6676, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127)." class="big_thumb" />

MAB37912). Cells were washed and stained with (A) Mouse Anti-Human CD4 Monoclonal Antibody (Catalog #MAB114341) or (B) Mouse IgG2B Isotype Control (MAB004) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B) and Mouse Anti-Human CD3 epsilon APC conjugated Monoclonal Antibody (FAB100A). This demonstrates non-competing epitopes of MAB114341 and MAB37912 which allows for purity analysis after CD4+ selection. View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox"> MAB37912). Cells were washed and stained with (A) Mouse Anti-Human CD4 Monoclonal Antibody (Catalog #MAB114341) or (B) Mouse IgG2B Isotype Control (MAB004) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B) and Mouse Anti-Human CD3 epsilon APC conjugated Monoclonal Antibody (FAB100A). This demonstrates non-competing epitopes of MAB114341 and MAB37912 which allows for purity analysis after CD4+ selection. View our protocol for Staining Membrane-associated Proteins. " alt="PBMC lymphocytes were stained with CD4 RPA-T4 (MAB37912). Cells were washed and stained with (A) Mouse Anti-Human CD4 Monoclonal Antibody (Catalog #MAB114341) or (B) Mouse IgG2B Isotype Control (MAB004) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B) and Mouse Anti-Human CD3 epsilon APC conjugated Monoclonal Antibody (FAB100A). This demonstrates non-competing epitopes of MAB114341 and MAB37912 which allows for purity analysis after CD4+ selection. View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

FAB114341P), and Mouse Anti-Human CD4 Alexa Fluor® 647‑conjugated Monoclonal Antibody (FAB37912R). This demonstrates non-competing epitopes of the two clones, which allows for purity analysis after CD4+ selection. View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox"> FAB114341P), and Mouse Anti-Human CD4 Alexa Fluor® 647‑conjugated Monoclonal Antibody (FAB37912R). This demonstrates non-competing epitopes of the two clones, which allows for purity analysis after CD4+ selection. View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />