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![Western Blot: APG8 Antibody [NB600-471] - Western blot using APG8 antibody generated by immunization with recombinant yeast APG8 vs other anti-UBL antibodies against E.coli lysates expressing APG8-GFP fusion protein. Panel A (top) total protein staining using ponceau. Panel B (bottom) specific reaction with APG8 using a 1:4,000 - 1:8,000 dilution of IgG fraction of Rabbit-anti-APG8 (Yeast) followed by 1:15,000 dilution of HRP Goat-a-Rabbit IgG MX. All primaries diluted in TTBS buffer with 5% non-fat milk and incubated with membranes overnight at 4C. E.coli lysate proteins separated by SDS-PAGE using 15% gel. Similar experiments (data not shown) using this antibody showed no reactivity of anti-APG8 with other UBLs, indicating that this antibody is highly specific and does not cross react with other UBLs. A chemiluminescence system was used for signal detection (Roche). Other detection systems will yield similar results.](http://images.novusbio.com/fullsize/APG8-Antibody-Western-Blot-NB600-471-img0008.jpg "Western Blot: APG8 Antibody [NB600-471] - Western blot using APG8 antibody generated by immunization with recombinant yeast APG8 vs other anti-UBL antibodies against E.coli lysates expressing APG8-GFP fusion protein. Panel A (top) total protein staining using ponceau. Panel B (bottom) specific reaction with APG8 using a 1:4,000 - 1:8,000 dilution of IgG fraction of Rabbit-anti-APG8 (Yeast) followed by 1:15,000 dilution of HRP Goat-a-Rabbit IgG MX. All primaries diluted in TTBS buffer with 5% non-fat milk and incubated with membranes overnight at 4C. E.coli lysate proteins separated by SDS-PAGE using 15% gel. Similar experiments (data not shown) using this antibody showed no reactivity of anti-APG8 with other UBLs, indicating that this antibody is highly specific and does not cross react with other UBLs. A chemiluminescence system was used for signal detection (Roche). Other detection systems will yield similar results.")
![N/A: APG8 Antibody [NB600-471]](http://images.novusbio.com/fullsize/APG8-Antibody-N-A-NB600-471-img0007.jpg)