| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61818-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48882-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-49368-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72303 | |||
![Western Blot: Lamin B2 Antibody (2E2F4) [NBP2-61818] - Analysis using LMNB2 mAb against HEK293 (1) and LMNB2 (AA: 401-600)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Lamin-B2-Antibody-2E2F4-Western-Blot-NBP2-61818-img0009.jpg "Western Blot: Lamin B2 Antibody (2E2F4) [NBP2-61818] - Analysis using LMNB2 mAb against HEK293 (1) and LMNB2 (AA: 401-600)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Lamin B2 Antibody (2E2F4) [NBP2-61818] - Analysis of HepG2 cells using LMNB2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Lamin-B2-Antibody-2E2F4-Immunocytochemistry-Immunofluorescence-NBP2-61818-img0005.jpg)
![Immunohistochemistry-Paraffin: Lamin B2 Antibody (2E2F4) [NBP2-61818] - Analysis of ovarian cancer tissues using LMNB2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Lamin-B2-Antibody-2E2F4-Immunohistochemistry-NBP2-61818-img0007.jpg)
![Flow Cytometry: Lamin B2 Antibody (2E2F4) [NBP2-61818] - Analysis of Hela cells using LMNB2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Lamin-B2-Antibody-2E2F4-Flow-Cytometry-NBP2-61818-img0002.jpg)
![Western Blot: Lamin B2 Antibody (2E2F4) [NBP2-61818] - Analysis using LMNB2 mAb against human LMNB2 (AA: 401-600) recombinant protein. (Expected MW is 47.6 kDa)](http://images.novusbio.com/fullsize/Lamin-B2-Antibody-2E2F4-Western-Blot-NBP2-61818-img0008.jpg)
![Western Blot: Lamin B2 Antibody (2E2F4) [NBP2-61818] - Analysis using LMNB2 mouse mAb against PC-3 (1), LNcap (2), Jurkat (3), and HCT116 (4) cell lysate.](http://images.novusbio.com/fullsize/Lamin-B2-Antibody-2E2F4-Western-Blot-NBP2-61818-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin B2 Antibody (2E2F4) [NBP2-61818] - Analysis of GC-7901 cells using LMNB2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Lamin-B2-Antibody-2E2F4-Immunocytochemistry-Immunofluorescence-NBP2-61818-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin B2 Antibody (2E2F4) [NBP2-61818] - Analysis of Hela cells using LMNB2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Lamin-B2-Antibody-2E2F4-Immunocytochemistry-Immunofluorescence-NBP2-61818-img0004.jpg)
![Immunohistochemistry-Paraffin: Lamin B2 Antibody (2E2F4) [NBP2-61818] - Analysis of colon cancer tissues using LMNB2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Lamin-B2-Antibody-2E2F4-Immunohistochemistry-NBP2-61818-img0006.jpg)
![ELISA: Lamin B2 Antibody (2E2F4) [NBP2-61818] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Lamin-B2-Antibody-2E2F4-ELISA-NBP2-61818-img0001.jpg)
![Western Blot: Lamin B2 Antibody (1S9M6) [NBP3-16527] - Western blot analysis of extracts of various cell lines, using Lamin B2 Rabbit mAb (NBP3-16527) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Lamin-B2-Antibody-1S9M6-Western-Blot-NBP3-16527-img0005.jpg "Western Blot: Lamin B2 Antibody (1S9M6) [NBP3-16527] - Western blot analysis of extracts of various cell lines, using Lamin B2 Rabbit mAb (NBP3-16527) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: Lamin B2 Antibody (1S9M6) [NBP3-16527] - Immunofluorescence analysis of U-2 OS cells using Lamin B2 Rabbit mAb (NBP3-16527) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Lamin-B2-Antibody-1S9M6-Immunocytochemistry-Immunofluorescence-NBP3-16527-img0004.jpg)
![Immunohistochemistry-Paraffin: Lamin B2 Antibody (1S9M6) [NBP3-16527] - Immunohistochemistry of paraffin-embedded mouse spinal cord using Lamin B2 Rabbit mAb (NBP3-16527) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Lamin-B2-Antibody-1S9M6-Immunohistochemistry-Paraffin-NBP3-16527-img0003.jpg)
![Immunohistochemistry-Paraffin: Lamin B2 Antibody (1S9M6) [NBP3-16527] - Immunohistochemistry of paraffin-embedded rat brain using Lamin B2 Rabbit mAb (NBP3-16527) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Lamin-B2-Antibody-1S9M6-Immunohistochemistry-Paraffin-NBP3-16527-img0001.jpg)
![Immunohistochemistry-Paraffin: Lamin B2 Antibody (1S9M6) [NBP3-16527] - Immunohistochemistry of paraffin-embedded human thyroid cancer using Lamin B2 Rabbit mAb (NBP3-16527) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Lamin-B2-Antibody-1S9M6-Immunohistochemistry-Paraffin-NBP3-16527-img0002.jpg)
![Immunohistochemistry: Lamin B2 Antibody (1S9M6) [NBP3-16527] - Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using Lamin B2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16527_rabbit-lamin-b2-mab-1s9m6-5220251255586.jpg)
![Immunohistochemistry: Lamin B2 Antibody (1S9M6) [NBP3-16527] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Lamin B2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16527_rabbit-lamin-b2-mab-1s9m6-5220251215479.jpg)
![Immunocytochemistry/ Immunofluorescence: Lamin B2 Antibody (1S9M6) [NBP3-16527] - Confocal imaging of HeLa cells using Lamin B2 Rabbit mAb . The cells were counterstained with alpha-Tubulin Rabbit mAb (Green). DAPI was used for nuclear staining (blue). Objective: 60x.](http://images.novusbio.com/fullsize/nbp3-16527_rabbit-lamin-b2-mab-1s9m6-5220251255584.jpg)
![Immunohistochemistry: Lamin B2 Antibody (1S9M6) [NBP3-16527] - Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using Lamin B2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16527_rabbit-lamin-b2-mab-1s9m6-52202511361229.jpg)
![Immunohistochemistry: Lamin B2 Antibody (1S9M6) [NBP3-16527] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Lamin B2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16527_rabbit-lamin-b2-mab-1s9m6-522025125449.jpg)
![Immunohistochemistry: Lamin B2 Antibody (1S9M6) [NBP3-16527] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Lamin B2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16527_rabbit-lamin-b2-mab-1s9m6-52202511374144.jpg)
![Immunohistochemistry: Lamin B2 Antibody (1S9M6) [Lamin B2] - Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using Lamin B2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16527_rabbit-lamin-b2-mab-1s9m6-52202517251913.jpg)
![Immunohistochemistry: Lamin B2 Antibody (1S9M6) [Lamin B2] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Lamin B2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16527_rabbit-lamin-b2-mab-1s9m6-52202517223922.jpg)
![Immunohistochemistry: Lamin B2 Antibody (1S9M6) [Lamin B2] - Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using Lamin B2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16527_rabbit-lamin-b2-mab-1s9m6-52202517284918.jpg)
![Immunohistochemistry: Lamin B2 Antibody (1S9M6) [Lamin B2] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Lamin B2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16527_rabbit-lamin-b2-mab-1s9m6-52202517273536.jpg)
![Immunocytochemistry/ Immunofluorescence: Lamin B2 Antibody (1S9M6) [Lamin B2] - Confocal imaging of HeLa cells using Lamin B2 Rabbit mAb . The cells were counterstained with alpha-Tubulin Rabbit mAb (Green). DAPI was used for nuclear staining (blue). Objective: 60x.](http://images.novusbio.com/fullsize/nbp3-16527_rabbit-lamin-b2-mab-1s9m6-52202517251939.jpg)
![Immunohistochemistry: Lamin B2 Antibody (1S9M6) [Lamin B2] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Lamin B2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16527_rabbit-lamin-b2-mab-1s9m6-5220251718919.jpg)
![Western Blot: Lamin B2 Antibody (144) [NBP2-43834] - Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin B2 antibody [144]. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Lamin-B2-Antibody-144-Western-Blot-NBP2-43834-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Lamin B2 Antibody (144) [NBP2-43834] - Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin B2 antibody [144]. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Lamin B2 Antibody (144) [NBP2-43834] - Analysis of HeLa cells were fixed in 2% paraformaldehyde for 30 min. Red: LMNB2 protein stained by Lamin B2 antibody [144] diluted at 1:1000. Green: alpha Tubulin antibody diluted at 1:1000. Blue: Hoechst 33342 staining. [Images captured by Olympus FV1000 Confocal Laser Scanning Microscope]](http://images.novusbio.com/fullsize/Lamin-B2-Antibody-144-Immunocytochemistry-Immunofluorescence-NBP2-43834-img0001.jpg)
![Immunohistochemistry-Paraffin: Lamin B2 Antibody (144) [NBP2-43834] - Analysis of Cal27 xenograft. Lamin B2 antibody [144] dilution: 1:200.](http://images.novusbio.com/fullsize/Lamin-B2-Antibody-144-Immunohistochemistry-Paraffin-NBP2-43834-img0002.jpg)
![Western Blot: Lamin B2 Antibody (144) [NBP2-43834] - Analysis of A. 30 ug HepG2 whole cell lysate/extract B. 30 ug HepG2 cytosol lysate/extract C. 30 ug HepG2 nuclear lysate/extract 7.5 % SDS-PAGE Lamin B2 antibody [144] dilution: 1:1000.](http://images.novusbio.com/fullsize/Lamin-B2-Antibody-144-Western-Blot-NBP2-43834-img0004.jpg)
![Western Blot: Lamin B2 Antibody (144) [NBP2-43834] - Analysis of A. 30 ug U87-MG whole cell lysate/extract B. 30 ug SK-N-SH whole cell lysate/extract C. 30 ug IMR32 whole cell lysate/extract D. 30 ug SK-N-AS whole cell lysate/extract 7.5 % SDS-PAGE Lamin B2 antibody [144] dilution: 1:1000](http://images.novusbio.com/fullsize/Lamin-B2-Antibody-144-Western-Blot-NBP2-43834-img0005.jpg)
![Immunoprecipitation: Lamin B2 Antibody (144) [NBP2-43834] - Analysis of HepG2 whole cell lysate/extract A : 30 ug whole cell lysate/extract of Lamin B2 protein expressing HepG2 cells B : Control with 3 ug of pre-immune mouse IgG C : Immunoprecipitation of Lamin B2 by 3 ug of Lamin B2 antibody [144] 7.5% SDS-PAGE The immunoprecipitated Lamin B2 protein was detected by Lamin B2 antibody [144] diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/Lamin-B2-Antibody-144-Immunoprecipitation-NBP2-43834-img0003.jpg)
![Immunohistochemistry-Paraffin: Lamin B2 Antibody [NBP1-33205] - Paraffin-embedded Cal27 xenograft. Lamin B2 antibody [N3C2], Internal dilution: 1:500](http://images.novusbio.com/fullsize/Lamin-B2-Antibody-Immunohistochemistry-Paraffin-NBP1-33205-img0012.jpg "Immunohistochemistry-Paraffin: Lamin B2 Antibody [NBP1-33205] - Paraffin-embedded Cal27 xenograft. Lamin B2 antibody [N3C2], Internal dilution: 1:500")
![Immunoprecipitation: Lamin B2 Antibody [NBP1-33205] - HepG2 whole cell lysate/extract A : 30 ug whole cell lysate/extract of Lamin beta2 protein expressing HepG2 cells B : Control with 3 ug of pre-immune rabbit IgG C : Immunoprecipitation of Lamin B2 by 3 ug of Lamin B2 antibody [N3C2], Internal 7.5% SDS-PAGE The immunoprecipitated Lamin B2 protein was detected by Lamin B2 antibody [N3C2], Internal diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/Lamin-B2-Antibody-Immunoprecipitation-NBP1-33205-img0006.jpg)
![Western Blot: Lamin B2 Antibody [NBP1-33205] -Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin B2 antibody [N3C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33205_rabbit-polyclonal-lamin-b2-antibody-western-blot-252023113644..jpg)
![Western Blot: Lamin B2 Antibody [NBP1-33205] - Lamin B2 antibody [N3C2], Internal detects LMNB2 protein by western blot analysis.A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extract C. 30 ug C8D30 whole cell lysate/extract D. 30 ug NIH-3T3 whole cell lysate/extract E. 30 ug BCL-1 lysate/extract F. 30 ug Raw264.7 whole cell lysate/extract G. 30 ug C2C12 whole cell lysate/extract7.5% SDS-PAGELamin B2 antibody [N3C2], Internal (NBP1-33205) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33205_rabbit-polyclonal-lamin-b2-antibody-91020242063343.jpg)
![Western Blot: Lamin B2 Antibody (J9-C4-R) [NBP3-32520] - Western blot analysis of Lamin B2 on different lysates with Mouse anti-Lamin B2 antibody (NBP3-32520) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: HCT 116 cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: COS-1 cell lysate (20 ug/Lane) Lane 5: Human brain tissue lysate (30 ug/Lane) Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32520) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32520_mouse-lamin-b2-mab-j9-c4-r-278202415242696.jpg "Western Blot: Lamin B2 Antibody (J9-C4-R) [NBP3-32520] - Western blot analysis of Lamin B2 on different lysates with Mouse anti-Lamin B2 antibody (NBP3-32520) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: HCT 116 cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: COS-1 cell lysate (20 ug/Lane) Lane 5: Human brain tissue lysate (30 ug/Lane) Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32520) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Lamin B2 Antibody (J9-C4-R) [NBP3-32520] - Immunofluorescence analysis of paraffin-embedded human brain tissue labeling Lamin B2 with Mouse anti-Lamin B2 antibody (NBP3-32520) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32520, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32520_mouse-lamin-b2-mab-j9-c4-r-298202414262635.jpg)
![Immunohistochemistry: Lamin B2 Antibody (J9-C4-R) [NBP3-32520] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-Lamin B2 antibody (NBP3-32520) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32520) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32520_mouse-lamin-b2-mab-j9-c4-r-29820241420943.jpg)
![Immunocytochemistry/ Immunofluorescence: Lamin B2 Antibody (J9-C4-R) [NBP3-32520] - Immunocytochemistry analysis of HepG2 cells labeling Lamin B2 with Mouse anti-Lamin B2 antibody (NBP3-32520) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Lamin B2 antibody (NBP3-32520) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32520_mouse-lamin-b2-mab-j9-c4-r-29202410484879.jpg)
![Western Blot: Lamin B2 Antibody [NBP2-19325] - Sample (30 ug of whole cell lysate) A: U87-MG 7. 5% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/Lamin-B2-Antibody-Western-Blot-NBP2-19325-img0001.jpg "Western Blot: Lamin B2 Antibody [NBP2-19325] - Sample (30 ug of whole cell lysate) A: U87-MG 7. 5% SDS PAGE gel, diluted at 1:10000.")
![Immunocytochemistry/Immunofluorescence: Lamin B2 Antibody [NBP2-19325] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Lamin B2 antibody (Green) at 1:500 dilution.](http://images.novusbio.com/fullsize/Lamin-B2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19325-img0003.jpg)
![Immunohistochemistry-Paraffin: Lamin B2 Antibody [NBP2-19325] - Immunohistochemical analysis of paraffin-embedded OVCA, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/Lamin-B2-Antibody-Immunohistochemistry-Paraffin-NBP2-19325-img0002.jpg)
![Western Blot: Lamin B2 Antibody (J9-C4) [NBP2-66923] - Analysis on Jurkat cell lysate using anti- LMNB2 mouse mAb.](http://images.novusbio.com/fullsize/Lamin-B2-Antibody-J9-C4-Western-Blot-NBP2-66923-img0001.jpg "Western Blot: Lamin B2 Antibody (J9-C4) [NBP2-66923] - Analysis on Jurkat cell lysate using anti- LMNB2 mouse mAb.")
![Immunohistochemistry-Paraffin: Lamin B2 Antibody (J9-C4) [NBP2-66923] - Analysis of paraffin-embedded human lung cancer tissue with Mouse anti-Lamin B2 antibody at 1/1000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Lamin-B2-Antibody-J9-C4-Immunohistochemistry-Paraffin-NBP2-66923-img0002.jpg)
![Western Blot: Lamin B2 Antibody (S02-9I7) [NBP3-15054] - Western blot detection of Lamin B2 in K562, Rat Brain, C6, 3T3, Hela cell lysates using Lamin B2 Rabbit mAb (1:1000 diluted). Predicted band size: 68kDa. Observed band size: 68kDa.](http://images.novusbio.com/fullsize/Lamin-B2-Antibody-S02-9I7-Western-Blot-NBP3-15054-img0001.jpg "Western Blot: Lamin B2 Antibody (S02-9I7) [NBP3-15054] - Western blot detection of Lamin B2 in K562, Rat Brain, C6, 3T3, Hela cell lysates using Lamin B2 Rabbit mAb (1:1000 diluted). Predicted band size: 68kDa. Observed band size: 68kDa.")
![Western Blot: Lamin B2 Antibody [NBP2-48882] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MGLane 4: Human plasmaLane 5: Human Liver tissueLane 6: Human Tonsil tissue](http://images.novusbio.com/fullsize/Lamin-B2-Antibody-Western-Blot-NBP2-48882-img0002.jpg "Western Blot: Lamin B2 Antibody [NBP2-48882] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MGLane 4: Human plasmaLane 5: Human Liver tissueLane 6: Human Tonsil tissue")
![Immunocytochemistry/Immunofluorescence: Lamin B2 Antibody [NBP2-49368] - Immunofluorescent staining of human cell line A-431 shows localization to nuclear membrane.](http://images.novusbio.com/fullsize/Lamin-B2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-49368-img0003.jpg "Immunocytochemistry/Immunofluorescence: Lamin B2 Antibody [NBP2-49368] - Immunofluorescent staining of human cell line A-431 shows localization to nuclear membrane.")
![Western Blot: Lamin B2 Antibody (2C6) [NBP2-45655] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Lamin B2.](http://images.novusbio.com/fullsize/Lamin-B2-Antibody-2C6-Western-Blot-NBP2-45655-img0001.jpg "Western Blot: Lamin B2 Antibody (2C6) [NBP2-45655] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Lamin B2.")
![Western Blot: Lamin B2 Overexpression Lysate (Adult Normal) [NBP2-05979] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Lamin B2.](http://images.novusbio.com/fullsize/Lamin-B2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-05979-img0001.jpg "Western Blot: Lamin B2 Overexpression Lysate (Adult Normal) [NBP2-05979] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Lamin B2.")