NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="Caspase-3 was detected in immersion fixed Jurkat human acute T cell leukemia cell line treated with Staurosporine using Rabbit Anti-Human Caspase-3 Monoclonal Antibody (Catalog # MAB7071) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB7071-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26699-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26698-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26461-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26463-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26462-50ul | |||
![Western Blot: Caspase-3 Antibody (1U1D9) - Active, Pro [NBP3-15840] - Western blot analysis of extracts of various cell lines, using Caspase-3 antibody (NBP3-15840) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-1U1D9-Active-Pro-Western-Blot-NBP3-15840-img0003.jpg "Western Blot: Caspase-3 Antibody (1U1D9) - Active, Pro [NBP3-15840] - Western blot analysis of extracts of various cell lines, using Caspase-3 antibody (NBP3-15840) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Western Blot: Caspase-3 Antibody (7T1W5) - Active, Pro [Caspase-3] - Western blot analysis of lysates from Jurkat cells, using [KO Validated] active + pro Caspase-3 Rabbit mAb at 1:1000 dilution. Jurkat cells were treated by Etoposide (25 uM) at 37C for 5 hours.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit .<br>Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-15840_rabbit-caspase-3-mab-7t1w5-active-pro-52202517251964.jpg)
![Western Blot: Caspase-3 Antibody (7T1W5) - Active, Pro [Caspase-3] - Western blot analysis of lysates from NIH/3T3 cells using Caspase-3 Rabbit mAb at 1:1000 dilution incubated overnight at 4C. NIH/3T3 cells were treated by staurosporine(1 uM) for 3 hour. <br> Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 30 ug per lane.<br> Blocking buffer: 3 % nonfat dry milk in TBST.<br> Detection: ECL Basic Kit .<br> Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-15840_rabbit-caspase-3-mab-7t1w5-active-pro-522025171898.jpg)
![Western Blot: Caspase-3 Antibody (7T1W5) - Active, Pro [Caspase-3] - Western blot analysis of lysates from L929 cells, using [KO Validated] active + pro Caspase-3 Rabbit mAb at 1:1000 dilution. L929 cells were treated by staurosporine(1 uM) for 3 hour.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit .<br>Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-15840_rabbit-caspase-3-mab-7t1w5-active-pro-52202517213580.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Caspase-3 Antibody (7T1W5) - Active, Pro [Caspase-3] - Western blot analysis of lysates from wild type (WT) and Caspase-3 knockout (KO) 293T cells using [KO Validated] Caspase-3 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25 ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit .<br>Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-15840_rabbit-caspase-3-mab-7t1w5-active-pro-52202517251998.jpg)
VC001) and DAB chromogen (brown). Tissues were counterstained with hematoxylin (blue)." class="big_thumb" />
![Immunohistochemistry: Caspase-3 Antibody (5B2) - Pro [NBP3-26699] - Image of Caspase-3 Antibody (5B2) - Pro diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26699_rabbit-caspase-3-mab-5b2-pro-262202415475638.jpg "Immunohistochemistry: Caspase-3 Antibody (5B2) - Pro [NBP3-26699] - Image of Caspase-3 Antibody (5B2) - Pro diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: Caspase-3 Antibody (5B2) - Pro [NBP3-26699] - Positive Western Blot detected in: Hela whole cell lysate, HepG2 whole cell lysate, HEK293 whole cell lysate, Ntera-2 whole cell lysate.<br /> All lanes: Caspase-3 Antibody - Pro at 1: 1000<br /> Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.<br /> Predicted band size: 32 kDa<br /> Observed band size: 32 kDa<br />](http://images.novusbio.com/fullsize/nbp3-26699_rabbit-caspase-3-mab-5b2-pro-28220241140943.jpg)
![Immunocytochemistry/Immunofluorescence: Caspase-3 Antibody (5B2) - Pro [NBP3-26699] - Staining of Hela Cells with Caspase-3 Antibody (5B2) - Pro at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26699_rabbit-caspase-3-mab-5b2-pro-2822024132361.jpg)
![Immunohistochemistry: Caspase-3 Antibody (14C1) [NBP3-26698] - Image of Caspase-3 Antibody (14C1) diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.18% DAB.](http://images.novusbio.com/fullsize/nbp3-26698_rabbit-caspase-3-mab-14c1-262202414471523.jpg "Immunohistochemistry: Caspase-3 Antibody (14C1) [NBP3-26698] - Image of Caspase-3 Antibody (14C1) diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.18% DAB.")
![Immunohistochemistry: Caspase-3 Antibody (14C1) [NBP3-26698] - Image of Caspase-3 Antibody (14C1) diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.18% DAB.](http://images.novusbio.com/fullsize/nbp3-26698_rabbit-caspase-3-mab-14c1-26220241447154.jpg)
![Western Blot: Caspase-3 Antibody (14C1) [NBP3-26698] - Positive Western Blot detected in: Hela whole cell lysate, HepG2 whole cell lysate, JK whole cell lysate, MCF7 whole cell lysate, K562 whole cell lysate, A549 whole cell lysate.<br /> All lanes: Caspase-3 Antibody at 1: 1000<br /> Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.<br /> Predicted band size: 32 kDa<br /> Observed band size: 32 kDa<br />](http://images.novusbio.com/fullsize/nbp3-26698_rabbit-caspase-3-mab-14c1-282202411442633.jpg)
![Immunohistochemistry: Caspase-3 Antibody (2B10) [NBP3-26461] - Image of Caspase-3 Antibody (2B10) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26461_rabbit-caspase-3-mab-2b10-262202414471514.jpg "Immunohistochemistry: Caspase-3 Antibody (2B10) [NBP3-26461] - Image of Caspase-3 Antibody (2B10) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: Caspase-3 Antibody (2B10) [NBP3-26461] - Positive Western Blot detected in: Hela whole cell lysate, HEK293 whole cell lysate, Ntera-2 whole cell lysate.<br /> All lanes: Caspase-3 Antibody at 1: 1000<br /> Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.<br /> Predicted band size: 32 kDa<br /> Observed band size: 32, 28 kDa<br />](http://images.novusbio.com/fullsize/nbp3-26461_rabbit-caspase-3-mab-2b10-282202411442613.jpg)
![Immunohistochemistry: Caspase-3 Antibody (6B2) - Pro [NBP3-26463] - Image of Caspase-3 Antibody (6B2) - Pro diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26463_rabbit-caspase-3-mab-6b2-pro-262202415475629.jpg "Immunohistochemistry: Caspase-3 Antibody (6B2) - Pro [NBP3-26463] - Image of Caspase-3 Antibody (6B2) - Pro diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: Caspase-3 Antibody (6B2) - Pro [NBP3-26463] - Positive Western Blot detected in: Ntera-2 whole cell lysate.<br /> All lanes: Caspase-3 Antibody - Pro at 1: 1000<br /> Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.<br /> Predicted band size: 32 kDa<br /> Observed band size: 32 kDa<br />](http://images.novusbio.com/fullsize/nbp3-26463_rabbit-caspase-3-mab-6b2-pro-282202411363021.jpg)
![Immunohistochemistry: Caspase-3 Antibody (3H12) - Active, Pro [NBP3-26462] - Image of Caspase-3 Antibody (3H12) - Active, Pro diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26462_rabbit-caspase-3-mab-3h12-active-pro-262202414471570.jpg "Immunohistochemistry: Caspase-3 Antibody (3H12) - Active, Pro [NBP3-26462] - Image of Caspase-3 Antibody (3H12) - Active, Pro diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: Caspase-3 Antibody (3H12) - Active, Pro [NBP3-26462] - Positive Western Blot detected in: Hela whole cell lysate, HEK293 whole cell lysate, Ntera-2 cell lysate.<br /> All lanes: Caspase-3 Antibody - Active, Pro at 1: 1000<br /> Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.<br /> Predicted band size: 32 kDa<br /> Observed band size: 32, 55, 130 kDa<br />](http://images.novusbio.com/fullsize/nbp3-26462_rabbit-caspase-3-mab-3h12-active-pro-28220241147218.jpg)
![Western Blot: Caspase-3 Antibody (S02-7C2) [NBP3-20021] - Western blot detection of pro Caspase-3 in K562, Hela cell lysates using NBP3-20021 (1:1000 diluted). Predicted band size: 32kDa. Observed band size: 32kDa.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-S02-7C2-Western-Blot-NBP3-20021-img0002.jpg "Western Blot: Caspase-3 Antibody (S02-7C2) [NBP3-20021] - Western blot detection of pro Caspase-3 in K562, Hela cell lysates using NBP3-20021 (1:1000 diluted). Predicted band size: 32kDa. Observed band size: 32kDa.")
![Immunohistochemistry-Paraffin: Caspase-3 Antibody (S02-7C2) [NBP3-20021] - Immunohistochemistry of Caspase-3 in paraffin-embedded Human lung cancer tissue using NBP3-20021 at dilution 1/20](http://images.novusbio.com/fullsize/Caspase-3-Antibody-S02-7C2-Immunohistochemistry-Paraffin-NBP3-20021-img0001.jpg)
