FAB4867A) and either (A) Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB170R) or (B) Mouse IgG2AIsotype Control (Catalog # MAB003) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." alt="Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB170R) or (B) Mouse IgG2AIsotype Control (Catalog # MAB003) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />
![Immunocytochemistry/Immunofluorescence: CXCR4 Antibody (2F10) [NBP3-15137] - Immunofluorescence staining of Hela Cells with CXCR4 antibody at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/CXCR4-Antibody-2F10-Immunocytochemistry-Immunofluorescence-NBP3-15137-img0001.jpg "Immunocytochemistry/Immunofluorescence: CXCR4 Antibody (2F10) [NBP3-15137] - Immunofluorescence staining of Hela Cells with CXCR4 antibody at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).")
350-NS) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # 
