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![Immunocytochemistry/Immunofluorescence: 14-3-3 beta Antibody (03) [NBP3-06516] - Staining of Human YWHAB in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human YWHAB monoclonal antibody (1:60) at 4? overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/14-3-3-beta-Antibody-03-Immunocytochemistry-Immunofluorescence-NBP3-06516-img0001.jpg "Immunocytochemistry/Immunofluorescence: 14-3-3 beta Antibody (03) [NBP3-06516] - Staining of Human YWHAB in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human YWHAB monoclonal antibody (1:60) at 4? overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.")
![Flow Cytometry: 14-3-3 beta Antibody (03) [NBP3-06516] - Analysis of Human YWHAB expression on Molt-4 cells. The cells were treated, stained with purified anti-Human YWHAB, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/14-3-3-beta-Antibody-03-Flow-Cytometry-NBP3-06516-img0002.jpg)
![Western Blot: 14-3-3 beta/alpha Antibody (2F7O1) [NBP3-16768] - Western blot analysis of extracts of various cell lines, using 14-3-3 beta/alpha Rabbit mAb (NBP3-16768) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-2F7O1-Western-Blot-NBP3-16768-img0001.jpg "Western Blot: 14-3-3 beta/alpha Antibody (2F7O1) [NBP3-16768] - Western blot analysis of extracts of various cell lines, using 14-3-3 beta/alpha Rabbit mAb (NBP3-16768) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: 14-3-3 beta/alpha Antibody (S07-1A3) [NBP3-19940] - Western blot detection of 14-3-3 beta/alpha in Jurkat, Rat Brain, C6, CHO-K1 cell lysates using NBP3-19940 (1:500 diluted). Predicted band size: 28kDa. Observed band size: 27-29kDa.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-S07-1A3-Western-Blot-NBP3-19940-img0001.jpg "Western Blot: 14-3-3 beta/alpha Antibody (S07-1A3) [NBP3-19940] - Western blot detection of 14-3-3 beta/alpha in Jurkat, Rat Brain, C6, CHO-K1 cell lysates using NBP3-19940 (1:500 diluted). Predicted band size: 28kDa. Observed band size: 27-29kDa.")
![Western Blot: 14-3-3 beta/alpha Overexpression Lysate (Adult Normal) [NBL1-17947] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for 14-3-3 beta/alpha.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17947-img0002.jpg "Western Blot: 14-3-3 beta/alpha Overexpression Lysate (Adult Normal) [NBL1-17947] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for 14-3-3 beta/alpha.")
![Western Blot: 14-3-3 beta/alpha Overexpression Lysate (Adult Normal) [NBL1-17946] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for 14-3-3 beta/alpha.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17946-img0002.jpg "Western Blot: 14-3-3 beta/alpha Overexpression Lysate (Adult Normal) [NBL1-17946] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for 14-3-3 beta/alpha.")
![SDS-Page: Recombinant Human 14-3-3 beta/alpha Protein [NBC1-18405] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.](http://images.novusbio.com/fullsize/Recombinant-Human-14-3-3-beta-alpha-Protein-SDS-Page-NBC1-18405-img0003.jpg "SDS-Page: Recombinant Human 14-3-3 beta/alpha Protein [NBC1-18405] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.")
![SDS-Page: Recombinant Human 14-3-3 beta/alpha Protein [NBP2-59657] - 15% SDS-PAGE (3ug)](http://images.novusbio.com/fullsize/Recombinant-Human-14-3-3-beta-alpha-Protein-SDS-Page-NBP2-59657-img0001.jpg "SDS-Page: Recombinant Human 14-3-3 beta/alpha Protein [NBP2-59657] - 15% SDS-PAGE (3ug)")