| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44979 | |||
| Azide and BSA Free | NBP2-34434-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB13552-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-59628 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48410 | |||
| Azide and BSA Free | NBP3-11546 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44977 | |||
| Azide and BSA Free | NBP2-34817-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1489-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44980 | |||
| Azide and BSA Free | NBP2-34563-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | IC13551A | |||
| Carrier Free | MAB13551-SP | |||
| Carrier Free | IC13551V-100UG | |||
| Carrier Free | IC13551U-100UG | |||
| Carrier Free | IC13551T-100UG | |||
| Carrier Free | IC13551S-100UG | |||
| Carrier Free | IC13551R-100UG | |||
| Carrier Free | IC13551N-100UG | |||
| Carrier Free | IC13551G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-59625 | |||
![Western Blot: p53 Antibody (SPM590) [NBP2-34387] - Western Blot Analysis of human HeLa cell lysate using p53 Antibody (SPM590).](http://images.novusbio.com/fullsize/p53-Antibody-SPM590-Western-Blot-NBP2-34387-img0003.jpg "Western Blot: p53 Antibody (SPM590) [NBP2-34387] - Western Blot Analysis of human HeLa cell lysate using p53 Antibody (SPM590).")
![Immunohistochemistry-Paraffin: p53 Antibody (SPM590) [NBP2-34387] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with P53 Monoclonal antibody (SPM590)](http://images.novusbio.com/fullsize/p53-Antibody-SPM590-Immunohistochemistry-Paraffin-NBP2-34387-img0002.jpg)
![ELISA p53 [p Ser15] [Biotin]](http://images.novusbio.com/fullsize2/dyc1839-2_human-phospho-p53-s15-duoset-ic-elisa-5-plate-elisa-169202013376.jpg "ELISA p53 [p Ser15] [Biotin]")
![Western Blot: p53 Antibody (TRP/816) [NBP2-59626] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p53 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-59626) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for p53 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/p53-Antibody-TRP-816-Knockout-Validated-NBP2-59626-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: p53 Antibody (TRP/816) [NBP2-59626] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p53 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-59626) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for p53 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Protein Array: p53 Antibody (TRP/816) [NBP2-59626] - containing more than 19,000 full-length human proteins. Z- and S- Score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary) produces when binding to a particular protein on the HuProt array. Z-scores are described in units of standard deviations above the mean value of all signals generated on that array. If targets on HuProt are arranged in descending order of the Z-score, the S-score is the difference between the Z-score. S-score therefore represents the relative target specificity of an Ab to its intended target. An Ab is considered to specific to its intended target, if the Ab has an S-score of at least 2.5. For example, if Ab binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Ab to protein X is equal to 29](http://images.novusbio.com/fullsize/p53-Antibody-TRP-816-Protein-Array-NBP2-59626-img0001.jpg)
![Western Blot: p53 Antibody (TRP/816) [NBP2-59626] - Western Blot Analysis of human HeLa cell lysate using p53 Antibody (TRP/816).](http://images.novusbio.com/fullsize/p53-Antibody-TRP-816-Western-Blot-NBP2-59626-img0006.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (TRP/816) [NBP2-59626] - Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with p53 Mouse Monoclonal Antibody (TRP/816).](http://images.novusbio.com/fullsize/p53-Antibody-TRP-816-Immunohistochemistry-Paraffin-NBP2-59626-img0002.jpg)
![Western Blot: p53 Antibody (TRP/817) [NBP2-44982] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p53 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-44982) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for p53 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/p53-Antibody-TRP-817-Knockout-Validated-NBP2-44982-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: p53 Antibody (TRP/817) [NBP2-44982] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p53 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-44982) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for p53 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: p53 Antibody (TRP/817) [NBP2-44982] - Western Blot Analysis of HeLa cell lysate using p53 Antibody (TRP/817).](http://images.novusbio.com/fullsize/p53-Antibody-TRP-817-Western-Blot-NBP2-44982-img0005.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (TRP/817) [NBP2-44982] - Human Colon Carcinoma stained with p53 Monoclonal Antibody (TRP/817)](http://images.novusbio.com/fullsize/p53-Antibody-TRP-817-Immunohistochemistry-Paraffin-NBP2-44982-img0001.jpg)
![Western Blot: p53 Antibody (SPM589) [NBP2-32891] - Western Blot Analysis of HeLa cell lysate using p53 Antibody (SPM589)](http://images.novusbio.com/fullsize/p53-Antibody-SPM589-Western-Blot-NBP2-32891-img0004.jpg "Western Blot: p53 Antibody (SPM589) [NBP2-32891] - Western Blot Analysis of HeLa cell lysate using p53 Antibody (SPM589)")
![Immunohistochemistry-Paraffin: p53 Antibody (SPM589) [NBP2-32891] - Formalin-fixed paraffin-embedded human bladder carcinoma stained with p53 monoclonal antibody (SPM589)](http://images.novusbio.com/fullsize/p53-Antibody-SPM589-Immunohistochemistry-Paraffin-NBP2-32891-img0003.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (BP53-12 + DO-7) [NBP2-34309] - Formalin-fixed, paraffin-embedded human cervical carcinoma stained with P53 Monoclonal antibody (BP53-12 +DO-7)](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-+-DO-7-Immunohistochemistry-Paraffin-NBP2-34309-img0002.jpg "Immunohistochemistry-Paraffin: p53 Antibody (BP53-12 + DO-7) [NBP2-34309] - Formalin-fixed, paraffin-embedded human cervical carcinoma stained with P53 Monoclonal antibody (BP53-12 +DO-7)")
![Protein Array: p53 Antibody (PAb122) [NBP2-59624] - containing more than 19,000 full-length human proteins. Z- and S- Score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary) produces when binding to a particular protein on the HuProt array. Z-scores are described in units of standard deviations above the mean value of all signals generated on that array. If targets on HuProt are arranged in descending order of the Z-score, the S-score is the difference between the Z-score. S-score therefore represents the relative target specificity of an Ab to its intended target. An Ab is considered to specific to its intended target, if the Ab has an S-score of at least 2.5. For example, if Ab binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Ab to protein X is equal to 29](http://images.novusbio.com/fullsize/p53-Antibody-PAb122-Protein-Array-NBP2-59624-img0001.jpg "Protein Array: p53 Antibody (PAb122) [NBP2-59624] - containing more than 19,000 full-length human proteins. Z- and S- Score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary) produces when binding to a particular protein on the HuProt array. Z-scores are described in units of standard deviations above the mean value of all signals generated on that array. If targets on HuProt are arranged in descending order of the Z-score, the S-score is the difference between the Z-score. S-score therefore represents the relative target specificity of an Ab to its intended target. An Ab is considered to specific to its intended target, if the Ab has an S-score of at least 2.5. For example, if Ab binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Ab to protein X is equal to 29")
![Western Blot: p53 Antibody (6B5-R) [NBP3-32698] - Western blot analysis of p53 on different lysates with Mouse anti-p53 antibody (NBP3-32698) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: MCF7 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 0.5uM doxorubicincell for 24 hours lysate Lane 5: Neuro-2a cell lysate Lane 6: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: Lane 1-2: 1 minute 40 seconds; Lane 3-6: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32698) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32698_mouse-p53-mab-6b5-r-278202415212411.jpg "Western Blot: p53 Antibody (6B5-R) [NBP3-32698] - Western blot analysis of p53 on different lysates with Mouse anti-p53 antibody (NBP3-32698) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: MCF7 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 0.5uM doxorubicincell for 24 hours lysate Lane 5: Neuro-2a cell lysate Lane 6: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: Lane 1-2: 1 minute 40 seconds; Lane 3-6: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32698) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: p53 Antibody (6B5-R) [NBP3-32698] - Immunocytochemistry analysis of A431 cells labeling p53 with Mouse anti-p53 antibody (NBP3-32698) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-p53 antibody (NBP3-32698) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32698_mouse-p53-mab-6b5-r-29202410484875.jpg)
![Immunoprecipitation: p53 Antibody (PSH03-95) [NBP3-32697] - p53 was immunoprecipitated in 0.2mg NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate with NBP3-32697 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32697 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate (input) Lane 2: NBP3-32697 IP in NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate Lane 3: Rabbit IgG instead of NBP3-32697 in NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes](http://images.novusbio.com/fullsize/nbp3-32697_rabbit-p53-mab-psh03-95-26820241582614.jpg "Immunoprecipitation: p53 Antibody (PSH03-95) [NBP3-32697] - p53 was immunoprecipitated in 0.2mg NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate with NBP3-32697 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32697 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate (input) Lane 2: NBP3-32697 IP in NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate Lane 3: Rabbit IgG instead of NBP3-32697 in NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes")
![Western Blot: p53 Antibody (PSH03-95) [NBP3-32697] - Western blot analysis of p53 on different lysates with Rabbit anti-p53 antibody (NBP3-32697) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: Neuro-2a cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 0.5uM doxorubicincell for 24 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 3 minutes 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32697) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32697_rabbit-p53-mab-psh03-95-278202415212448.jpg)
![Immunocytochemistry/ Immunofluorescence: p53 Antibody (PSH03-95) [NBP3-32697] - Immunocytochemistry analysis of NIH/3T3 cells treated with 0.5μM doxorubicin for 20 hours labeling p53 with Rabbit anti-p53 antibody (NBP3-32697) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p53 antibody (NBP3-32697) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32697_rabbit-p53-mab-psh03-95-2920241048481.jpg)