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![Immunocytochemistry/ Immunofluorescence: Cardiac Troponin T Antibody (10H9D8) [NBP3-33537] - Immunofluorescence analysis of paraffin-embedded mouse heart using Cardiac Troponin T(TNNT2) Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-33537_rabbit-cardiac-troponin-t-mab-10h9d8-912202415161863.jpg "Immunocytochemistry/ Immunofluorescence: Cardiac Troponin T Antibody (10H9D8) [NBP3-33537] - Immunofluorescence analysis of paraffin-embedded mouse heart using Cardiac Troponin T(TNNT2) Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunocytochemistry/ Immunofluorescence: Cardiac Troponin T Antibody (10H9D8) [NBP3-33537] - Immunofluorescence analysis of paraffin-embedded rat heart using Cardiac Troponin T Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-33537_rabbit-cardiac-troponin-t-mab-10h9d8-3112025170473.jpg)
![Immunohistochemistry: Rabbit Cardiac Troponin T mAb (10H9D8) [NBP3-33537] - Immunofluorescence analysis of paraffin-embedded mouse heart using Cardiac troponin T (TNNT2) Rabbit mAb (A4914) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/antibody/nbp3-33537_rabbit-cardiac-troponin-t-mab-10h9d8-immunohistochemistry-322025183725..jpg)
![Sandwich ELISA: Troponin T Type 2 (cardiac) Antibody (1C11) [NB200-412] - Calibrating curves for sandwich cTnT fluoroimmunoassay with different animal TnTs as antigen: human, canine, rat, mouse Clone 1C11 for capture (1 ug/well) and Clone b 7E7 for detection (200 ng/well). Assay time: 30 min. Room temperature. Hybridoma cell line producing Clone 7E7 was raised after hybridization of myeloma cell line Sp2/0 with splenocytes of mice immunized with human tertiary Tn complex. Thus Clone 7E7 demonstrates slight cross-reaction with cTnI (about 5% based on ELISA data).](http://images.novusbio.com/fullsize/Troponin-T-Type-2-cardiac-Antibody-1C11-Sandwich-ELISA-NB200-412-img0002.jpg "Sandwich ELISA: Troponin T Type 2 (cardiac) Antibody (1C11) [NB200-412] - Calibrating curves for sandwich cTnT fluoroimmunoassay with different animal TnTs as antigen: human, canine, rat, mouse Clone 1C11 for capture (1 ug/well) and Clone b 7E7 for detection (200 ng/well). Assay time: 30 min. Room temperature. Hybridoma cell line producing Clone 7E7 was raised after hybridization of myeloma cell line Sp2/0 with splenocytes of mice immunized with human tertiary Tn complex. Thus Clone 7E7 demonstrates slight cross-reaction with cTnI (about 5% based on ELISA data).")
![Western Blot: Cardiac Troponin T Antibody [NBP3-13030] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membranes were blotted with Cardiac Troponin T antibody (NBP3-13030) diluted at 1:3000 and competitor's antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cardiac-Troponin-T-Antibody-Western-Blot-NBP3-13030-img0003.jpg "Western Blot: Cardiac Troponin T Antibody [NBP3-13030] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membranes were blotted with Cardiac Troponin T antibody (NBP3-13030) diluted at 1:3000 and competitor's antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: Cardiac Troponin T Antibody [NBP3-13030] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cardiac Troponin T antibody (NBP3-13030) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cardiac-Troponin-T-Antibody-Western-Blot-NBP3-13030-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Cardiac Troponin T Antibody [NBP3-13030] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membranes were blotted with Cardiac Troponin T antibody (NBP3-13030) diluted at 1:3000 and competitor's antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Cardiac-Troponin-T-Antibody-Western-Blot-NBP3-13030-img0002.jpg)
![Immunohistochemistry: Troponin T Type 2 (cardiac) Antibody (8H8) [NBP3-26279] - Image of Troponin T Type 2 (cardiac) Antibody (8H8) diluted at 1:50 and staining in paraffin-embedded human heart tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.16% DAB.](http://images.novusbio.com/fullsize/nbp3-26279_rabbit-troponin-t-type-2-cardiac-mab-8h8-27220248313717.jpg "Immunohistochemistry: Troponin T Type 2 (cardiac) Antibody (8H8) [NBP3-26279] - Image of Troponin T Type 2 (cardiac) Antibody (8H8) diluted at 1:50 and staining in paraffin-embedded human heart tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.16% DAB.")
![Immunohistochemistry: Troponin T Type 2 (cardiac) Antibody (8H8) [NBP3-26279] - Image of Troponin T Type 2 (cardiac) Antibody (8H8) diluted at 1:50 and staining in paraffin-embedded human heart tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.16% DAB.](http://images.novusbio.com/fullsize/nbp3-26279_rabbit-troponin-t-type-2-cardiac-mab-8h8-2722024831371.jpg)
![Flow Cytometry: Troponin T Type 2 (cardiac) Antibody (8H8) [NBP3-26279] - Overlay Peak curve showing MCF-7 cells stained with Troponin T Type 2 (cardiac) Antibody (8H8) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26279_rabbit-troponin-t-type-2-cardiac-mab-8h8-282202414183674.jpg)