1399-IL) induces CXCL1/GROa secretion in the the HT-29 human colon adenocarcinoma cell line in a dose-dependent manner (orange line), as measured by Human CXCL1/GROa DuoSet (Catalog # DY275). CXCL1/GROa Secretion elicited by Recombinant Mouse IL-17E/IL-25 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-17E/IL-25 Monoclonal Antibody (Catalog # MAB13992). The ND50 is typically 0.005-0.025 µg/mL." alt="Recombinant Mouse IL-17E/IL-25 (Catalog # 1399-IL) induces CXCL1/GROa secretion in the the HT-29 human colon adenocarcinoma cell line in a dose-dependent manner (orange line), as measured by Human CXCL1/GROa DuoSet (Catalog # DY275). CXCL1/GROa Secretion elicited by Recombinant Mouse IL-17E/IL-25 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-17E/IL-25 Monoclonal Antibody (Catalog # MAB13992). The ND50 is typically 0.005-0.025 µg/mL." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB13992-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27324SS | |||
| Azide and BSA Free | NBP2-80795 | |||
MAB12582) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human IL-17E/IL-25 Monoclonal Antibody (Catalog # MAB12581) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Human IL-17E/IL-25 protein was serially diluted 2-fold and captured by Mouse Anti-Human IL-17E/IL-25 Monoclonal Antibody (Catalog # MAB12582) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human IL-17E/IL-25 Monoclonal Antibody (Catalog # MAB12581) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB12581-SP | |||
DY990). Mouse Anti-Human IL-17E/IL-25 Monoclonal Antibody (Catalog # MAB12581) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Human IL-17E/IL-25 protein was serially diluted 2-fold and captured by Mouse Anti-Human IL-17E/IL-25 Monoclonal Antibody (Catalog # MAB12582) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human IL-17E/IL-25 Monoclonal Antibody (Catalog # MAB12581) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB12582-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | 8949-IL-025/CF | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14176 | |||
![Western Blot: IL-17E/IL-25 Antibody (8B1) [NBP2-27324] - Analysis of IL17E in full-length recombinant protein using IL17E antibody at 0.5 ug/ml.](http://images.novusbio.com/fullsize/IL-17E-IL-25-Antibody-8B1-Western-Blot-NBP2-27324-img0004.jpg "Western Blot: IL-17E/IL-25 Antibody (8B1) [NBP2-27324] - Analysis of IL17E in full-length recombinant protein using IL17E antibody at 0.5 ug/ml.")
![Flow Cytometry: IL-17E/IL-25 Antibody (8B1) [NBP2-27324] - Intracellular analysis of IL-17E in PC3 cells using 1 ug of IL-17E antibody (green histogram represents isotype control antibody this antibody , red represents IL-17E antibody. this antibody was used for this test, and an anti-mouse IgG PE conjugated secondary antibody this antibody .](http://images.novusbio.com/fullsize/IL-17E-IL-25-Antibody-8B1-Flow-Cytometry-NBP2-27324-img0003.jpg)
![ELISA: Human IL-17E/IL-25 ELISA Kit (Colorimetric) [NBP2-68035] - Samples were spiked with high concentrations of Human IL-17E/IL-25 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-68035_human-il-17e-il-25-elisa-kit-colorimetric-132202416121370.jpg "ELISA: Human IL-17E/IL-25 ELISA Kit (Colorimetric) [NBP2-68035] - Samples were spiked with high concentrations of Human IL-17E/IL-25 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human IL-17E/IL-25 ELISA Kit (Colorimetric) [NBP2-68035] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-68035_human-il-17e-il-25-elisa-kit-colorimetric-432024932656.jpg)
![Immunocytochemistry/Immunofluorescence: IL-17E/IL-25 Antibody (RM0263-5J46) [NBP2-11677] - Immunofluorescence for IL25-positive cells (red), actin (green) and nuclei (blue) in lung tissue from control mice. The control mice had no experimental manipulation performed. C57BL/6 male mice (Jackson Laboratories, Bar Harbor, Me), 8 to 12 weeks old and weighing 20 to 30 g, were used in this study. Samples were imaged on a FluoView 1000 confocal scanning microscope (Olympus). Imaging conditions were maintained at identical settings within each antibody-labeling experiment with original gating performed using the negative control (no primary antibody). Image courtesy of Center for Biologic Imaging, The University of Pittsburgh](http://images.novusbio.com/fullsize/IL-17E-IL-25-Antibody-RM0263-5J46-Immunocytochemistry-Immunofluorescence-NBP2-11677-img0003.jpg "Immunocytochemistry/Immunofluorescence: IL-17E/IL-25 Antibody (RM0263-5J46) [NBP2-11677] - Immunofluorescence for IL25-positive cells (red), actin (green) and nuclei (blue) in lung tissue from control mice. The control mice had no experimental manipulation performed. C57BL/6 male mice (Jackson Laboratories, Bar Harbor, Me), 8 to 12 weeks old and weighing 20 to 30 g, were used in this study. Samples were imaged on a FluoView 1000 confocal scanning microscope (Olympus). Imaging conditions were maintained at identical settings within each antibody-labeling experiment with original gating performed using the negative control (no primary antibody). Image courtesy of Center for Biologic Imaging, The University of Pittsburgh")
![Immunohistochemistry-Paraffin: IL-17E/IL-25 Antibody (IL25/625) [NBP3-13773] - Formalin-fixed, paraffin-embedded human adrenalgland stained with IL-17E/IL-25 antibody (IL25/625). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/IL-17E-IL-25-Antibody-IL25-625-Immunohistochemistry-Paraffin-NBP3-13773-img0001.jpg "Immunohistochemistry-Paraffin: IL-17E/IL-25 Antibody (IL25/625) [NBP3-13773] - Formalin-fixed, paraffin-embedded human adrenalgland stained with IL-17E/IL-25 antibody (IL25/625). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.")
![Immunohistochemistry-Paraffin: IL-17E/IL-25 Antibody (IL25/625) [NBP3-13773] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with IL-17E/IL-25 antibody (IL25/625). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/IL-17E-IL-25-Antibody-IL25-625-Immunohistochemistry-Paraffin-NBP3-13773-img0002.jpg)
![ELISA: Mouse IL-17E/IL-25 ELISA Kit (Colorimetric) [NBP3-06745] - Samples were spiked with high concentrations of Mouse IL-17E/IL-25 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-06745_mouse-il-17e-il-25-elisa-kit-colorimetric-132202416235781.jpg "ELISA: Mouse IL-17E/IL-25 ELISA Kit (Colorimetric) [NBP3-06745] - Samples were spiked with high concentrations of Mouse IL-17E/IL-25 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse IL-17E/IL-25 ELISA Kit (Colorimetric) [NBP3-06745] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-06745_mouse-il-17e-il-25-elisa-kit-colorimetric-4320249133223.jpg)
![ELISA: Rat IL-17E/IL-25 ELISA Kit (Colorimetric) [NBP3-06783] - Samples were spiked with high concentrations of Rat IL-17E/IL-25 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-06783_rat-il-17e-il-25-elisa-kit-colorimetric-13220241626151.jpg "ELISA: Rat IL-17E/IL-25 ELISA Kit (Colorimetric) [NBP3-06783] - Samples were spiked with high concentrations of Rat IL-17E/IL-25 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat IL-17E/IL-25 ELISA Kit (Colorimetric) [NBP3-06783] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-06783_rat-il-17e-il-25-elisa-kit-colorimetric-43202495084.jpg)
![Western Blot: IL17E Overexpression Lysate (Adult Normal) [NBL1-11946] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IL17E.](http://images.novusbio.com/fullsize/IL17E-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11946-img0002.jpg "Western Blot: IL17E Overexpression Lysate (Adult Normal) [NBL1-11946] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IL17E.")
