575-ES) was serially diluted and captured by Rat Anti-Mouse E‑Selectin/CD62E Monoclonal Antibody (Catalog # MAB5751) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse E‑Selectin/CD62E Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" alt="Recombinant Mouse E‑Selectin/CD62E Fc Chimera (Catalog # 575-ES) was serially diluted and captured by Rat Anti-Mouse E‑Selectin/CD62E Monoclonal Antibody (Catalog # MAB5751) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse E‑Selectin/CD62E Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB5751-SP | |||
| Carrier Free | MAB5751-500 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF977-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF724-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB575-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-43393-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89432-50ul | |||
![Flow Cytometry: E-selectin Antibody (P2H3) [NBP1-43393] - Staining of TNF alpha-stimulated Human Umbilical Vein Endothelial Cells (HUVEC) with 0.5 ug of Mouse IgG1 kappa Isotype Control Purified (open histogram) or 0.5 ug of Anti-Human CD62E (E- Selectin) Purified (filled histogram) followed by F(ab')2 Anti-Mouse IgG PE . Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/E-selectin-Antibody-P2H3-Flow-Cytometry-NBP1-43393-img0001.jpg "Flow Cytometry: E-selectin Antibody (P2H3) [NBP1-43393] - Staining of TNF alpha-stimulated Human Umbilical Vein Endothelial Cells (HUVEC) with 0.5 ug of Mouse IgG1 kappa Isotype Control Purified (open histogram) or 0.5 ug of Anti-Human CD62E (E- Selectin) Purified (filled histogram) followed by F(ab')2 Anti-Mouse IgG PE . Total viable cells were used for analysis.")
![Immunocytochemistry/Immunofluorescence: E-Selectin/CD62E Antibody (208) [NBP2-89432] - Staining of CD62E in TNF-a stimulated HUVEC cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-89432 antibody (1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cells membrane.](http://images.novusbio.com/fullsize/nbp2-89432_rabbit-monoclonal-e-selectin-cd62e-antibody-208-235202514371334.png "Immunocytochemistry/Immunofluorescence: E-Selectin/CD62E Antibody (208) [NBP2-89432] - Staining of CD62E in TNF-a stimulated HUVEC cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-89432 antibody (1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cells membrane.")
![Western Blot: E-selectin Overexpression Lysate (Adult Normal) [NBL1-15790] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for E-selectin.](http://images.novusbio.com/fullsize/E-selectin-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-15790-img0002.jpg "Western Blot: E-selectin Overexpression Lysate (Adult Normal) [NBL1-15790] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for E-selectin.")
