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![Western Blot: Ribosomal Protein S6/RPS6 [p Ser235] Antibody [NBP3-13078] - Untreated (-) and treated (+) 293T whole cell extracts starvation for 16 hrs (40 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ribosomal Protein S6/RPS6 (phospho Ser235) antibody (NBP3-13078) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Ribosomal-Protein-S6-RPS6-[p-Ser235]-Antibody-Western-Blot-NBP3-13078-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: Ribosomal Protein S6/RPS6 [p Ser235] Antibody [NBP3-13078] - Untreated (-) and treated (+) 293T whole cell extracts starvation for 16 hrs (40 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ribosomal Protein S6/RPS6 (phospho Ser235) antibody (NBP3-13078) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Ribosomal Protein S6/RPS6 [p Ser235] Antibody [NBP3-13078] - Ribosomal Protein S6/RPS6 (phospho Ser235) antibody detects Ribosomal Protein S6/RPS6 (phospho Ser235) protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Ribosomal Protein S6/RPS6 (phospho Ser235) antibody (NBP3-13078) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Ribosomal-Protein-S6-RPS6-[p-Ser235]-Antibody-Immunohistochemistry-Paraffin-NBP3-13078-img0001.jpg)
![Western Blot: Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody [NB100-1553] - Detection of Human Phospho RPS6 (S235/S236) by Western Blot. Samples: Whole cell lysate (35 mcg) from HeLa cells. Treatments consisted of growth in serum containing media (lane 1) or cultured for 24 hours in serum free media (lanes 2 to 5). At 24h, serum was replaced for 1 h (lanes 2 and 3) or serum starvation was continued (lanes 4 and 5) without rapamycin (lanes 3 and 5) with 50nM Rapamcycin (lanes 2 and 4). Antibodies: Affinity purified rabbit anti-phospho RPS6 (S235/S236) antibody NB100-1553 used at 0.04 mcg/ml (upper panel). The blot was stripped and re-blotted for total RPS6 using affinity purified rabbit anti-RPS6 antibody NB100-1595 at 0.04 mcg/ml. Detection: Chemiluminescence with exposure times of 10 seconds (upper panel) or 30 seconds (lower panel).](http://images.novusbio.com/fullsize/Ribosomal-Protein-S6-RPS6-[p-Ser235--p-Ser236]-Antibody-Western-Blot-NB100-1553-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody [NB100-1553] - Detection of Human Phospho RPS6 (S235/S236) by Western Blot. Samples: Whole cell lysate (35 mcg) from HeLa cells. Treatments consisted of growth in serum containing media (lane 1) or cultured for 24 hours in serum free media (lanes 2 to 5). At 24h, serum was replaced for 1 h (lanes 2 and 3) or serum starvation was continued (lanes 4 and 5) without rapamycin (lanes 3 and 5) with 50nM Rapamcycin (lanes 2 and 4). Antibodies: Affinity purified rabbit anti-phospho RPS6 (S235/S236) antibody NB100-1553 used at 0.04 mcg/ml (upper panel). The blot was stripped and re-blotted for total RPS6 using affinity purified rabbit anti-RPS6 antibody NB100-1595 at 0.04 mcg/ml. Detection: Chemiluminescence with exposure times of 10 seconds (upper panel) or 30 seconds (lower panel).")
![Western Blot: Ribosomal Protein S6/RPS6 [p Ser235] Antibody (GT829) [NBP3-13571] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ribosomal Protein S6/RPS6 (phospho Ser235) antibody [GT829] (NBP3-13571) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Ribosomal-Protein-S6-RPS6-p-Ser235-Antibody-GT829-Western-Blot-NBP3-13571-img0002.jpg "Western Blot: Ribosomal Protein S6/RPS6 [p Ser235] Antibody (GT829) [NBP3-13571] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ribosomal Protein S6/RPS6 (phospho Ser235) antibody [GT829] (NBP3-13571) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Biological Strategies Validation. </b>Western Blot: Ribosomal Protein S6/RPS6 [p Ser235] Antibody (GT829) [NBP3-13571] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ribosomal Protein S6/RPS6 (phospho Ser235) antibody [GT829] (NBP3-13571) diluted at 1:1000.](http://images.novusbio.com/fullsize/Ribosomal-Protein-S6-RPS6-p-Ser235-Antibody-GT829-Western-Blot-NBP3-13571-img0001.jpg)
![Western Blot: Ribosomal Protein S6/RPS6 Antibody (A6B8-R) [NBP3-32919] - Western blot analysis of Ribosomal Protein S6/RPS6 on different lysates with Mouse anti-Ribosomal Protein S6/RPS6 antibody (NBP3-32919) at 1/1,000 dilution.<br/><br/> Lane 1: HeLa cell lysate<br/> Lane 2: MCF7 cell lysate<br/> Lane 3: HepG2 cell lysate<br/> Lane 4: HEK-293 cell lysate<br/> Lane 5: HCT 116 cell lysate<br/> Lane 6: THP-1 cell lysate<br/> Lane 7: NIH/3T3 cell lysate<br/> Lane 8: PC-12 cell lysate<br/><br/> Lysates/proteins at 20 ug/Lane.<br/><br/> Predicted band size: 29 kDa<br/> Observed band size: 29 kDa<br/><br/> Exposure time: 1 minute 22 seconds;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32919) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32919_mouse-ribosomal-protein-s6-rps6-mab-a6b8-r-288202412461617.jpg "Western Blot: Ribosomal Protein S6/RPS6 Antibody (A6B8-R) [NBP3-32919] - Western blot analysis of Ribosomal Protein S6/RPS6 on different lysates with Mouse anti-Ribosomal Protein S6/RPS6 antibody (NBP3-32919) at 1/1,000 dilution.<br/><br/> Lane 1: HeLa cell lysate<br/> Lane 2: MCF7 cell lysate<br/> Lane 3: HepG2 cell lysate<br/> Lane 4: HEK-293 cell lysate<br/> Lane 5: HCT 116 cell lysate<br/> Lane 6: THP-1 cell lysate<br/> Lane 7: NIH/3T3 cell lysate<br/> Lane 8: PC-12 cell lysate<br/><br/> Lysates/proteins at 20 ug/Lane.<br/><br/> Predicted band size: 29 kDa<br/> Observed band size: 29 kDa<br/><br/> Exposure time: 1 minute 22 seconds;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32919) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: RPS6 [p Ser244] Antibody [NBP2-29653] - Jurkat cell lysate showing specific immunolabeling of the ~28k rpS6 phosphorylated at Ser244 (Control). Phosphospecificity is shown in the second lane where immunolabeling is blocked by preadsorption of the phospho-peptide used as antigen (peptide) but not by the corresponding dephosphopeptide (not shown).](http://images.novusbio.com/fullsize/RPS6-[p-Ser244]-Antibody-Western-Blot-NBP2-29653-img0001.jpg "Western Blot: RPS6 [p Ser244] Antibody [NBP2-29653] - Jurkat cell lysate showing specific immunolabeling of the ~28k rpS6 phosphorylated at Ser244 (Control). Phosphospecificity is shown in the second lane where immunolabeling is blocked by preadsorption of the phospho-peptide used as antigen (peptide) but not by the corresponding dephosphopeptide (not shown).")