7898-TGB) with an ED50 of 4.00‑60.0 ng/mL." alt="Biotinylated Recombinant Human CD155/PVR His-tag Protein (Catalog # BT2530) binds Recombinant Human TIGIT (A103) Fc Chimera (7898-TGB) with an ED50 of 4.00‑60.0 ng/mL." class="big_thumb" />
9556-CD) binds with an ED50 of 0.25-1.25 µg/mL." alt="When Recombinant Cynomolgus Monkey CD155/PVR Fc Chimera (Catalog # 10058-CD) is coated at 5 µg/mL, 100 µL/well, Recombinant Human CD96v2 Fc Chimera (Catalog # 9556-CD) binds with an ED50 of 0.25-1.25 µg/mL." class="big_thumb" />
9556-CD) is immobilized at 1 µg/mL, Biotinylated Recombinant Human CD155/PVR Fc Chimera Avi-tag (Catalog # AVI9174) binds with an ED50 of 10-60 ng/mL." alt="When Recombinant Human CD96v2 Fc Chimera (Catalog # 9556-CD) is immobilized at 1 µg/mL, Biotinylated Recombinant Human CD155/PVR Fc Chimera Avi-tag (Catalog # AVI9174) binds with an ED50 of 10-60 ng/mL." class="big_thumb" />
5690-CD) binds with an ED50 of 1.5-7.5 ng/mL." alt="When Recombinant Rat CD155/PVR (Catalog # 9764-CD) is immobilized at 1 µg/mL, 100 µL/well, Recombinant Mouse CD96 (Catalog # 5690-CD) binds with an ED50 of 1.5-7.5 ng/mL." class="big_thumb" />
5690-CD) is coated at 1 µg/mL, 100 µL/well, Recombinant Rat CD155/PVR Fc Chimera (Catalog # 9744-CD) binds with an ED50 of 1-8 ng/mL." alt="When Recombinant Mouse CD96 (Catalog # 5690-CD) is coated at 1 µg/mL, 100 µL/well, Recombinant Rat CD155/PVR Fc Chimera (Catalog # 9744-CD) binds with an ED50 of 1-8 ng/mL." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | 6909-CD-01M | |||
![Flow Cytometry: CD155/PVR Antibody (SKII.4) - Chimeric - BSA Free [NBP3-45129] - Paraformaldehyde-fixed Jurkat cells permeabilized with 0.5% Triton were stained with the anti-unknown specificity antibody (isotype control, black line) or NBP3-45129 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000, and the cells were analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-49155_rabbit-xk-x-linked-kx-blood-group-pab-azide-and-bsa-free-immunocytochemistry-immunofluorescence-2220260311822.png "Flow Cytometry: CD155/PVR Antibody (SKII.4) - Chimeric - BSA Free [NBP3-45129] - Paraformaldehyde-fixed Jurkat cells permeabilized with 0.5% Triton were stained with the anti-unknown specificity antibody (isotype control, black line) or NBP3-45129 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000, and the cells were analyzed using a FACSCanto flow-cytometer.")
![Immunocytochemistry/ Immunofluorescence: CD155/PVR Antibody (SKII.4) - Chimeric - BSA Free [NBP3-45129] - Immunofluorescence analysis of paraformaldehyde-fixed A431 cells on coverslips stained with NBP3-45129 (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic and membrane staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, NBP3-45129, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-45129_rabbit-cd155-pvr-mab-skii-4-chimeric-bsa-free-immunocytochemistry-immunofluorescence-222026035555.png)
![N/A CD155/PVR [Biotin]](http://images.novusbio.com/fullsize/dy2530-05_human-cd155-pvr-duoset-elisa-5-plate-1920221020.png "N/A CD155/PVR [Biotin]")
![Western Blot: CD155/PVR Antibody (JE45-53) [NBP3-32840] - Western blot analysis of CD155/PVR on different lysates with Rabbit anti-CD155/PVR antibody (NBP3-32840) at 1/1,000 dilution.<br/><br/> Lane 1: HUVEC cell lysate<br/> Lane 2: U87-MG cell lysate<br/><br/> Lysates/proteins at 20 ug/Lane.<br/><br/> Predicted band size: 45 kDa<br/> Observed band size: 75 kDa<br/><br/> Exposure time: 4 minutes 39 seconds;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32840) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32840_rabbit-cd155-pvr-mab-je45-53-28820241244292.jpg "Western Blot: CD155/PVR Antibody (JE45-53) [NBP3-32840] - Western blot analysis of CD155/PVR on different lysates with Rabbit anti-CD155/PVR antibody (NBP3-32840) at 1/1,000 dilution.<br/><br/> Lane 1: HUVEC cell lysate<br/> Lane 2: U87-MG cell lysate<br/><br/> Lysates/proteins at 20 ug/Lane.<br/><br/> Predicted band size: 45 kDa<br/> Observed band size: 75 kDa<br/><br/> Exposure time: 4 minutes 39 seconds;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32840) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: CD155/PVR Antibody (JE45-53) [NBP3-32840] - Immunocytochemistry analysis of HUVEC cells labeling CD155/PVR with Rabbit anti-CD155/PVR antibody (NBP3-32840) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CD155/PVR antibody (NBP3-32840) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32840_rabbit-cd155-pvr-mab-je45-53-29202410234257.jpg)
![Western blot analysis of A549 cell lysates with NBP3-32840 at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 45 kDa Observed band size: 75 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32840 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32840_-western-blot-639204597795707562.jpg)
