Products Related to Gene-ID: 5728

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="PTEN was detected in immersion fixed HeLa cells (positive) HeLa KO (negative) using Mouse Anti-Human PTEN Monoclonal Antibody (Catalog # MAB66551) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

MAB0041, open histogram), followed by PE-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our protocol for Staining Intracellular Molecules." class="big_lightbox"> MAB0041, open histogram), followed by PE-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our protocol for Staining Intracellular Molecules." alt=" Human U937 histiocytic lymphoma cell line was stained with Mouse Anti-Human/Mouse PTEN Monoclonal Antibody (Catalog # MAB6655, filled histogram) or isotype control antibody (MAB0041, open histogram), followed by PE-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our protocol for Staining Intracellular Molecules." class="big_thumb" />