| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB66551-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | IC6655V-100UG | |||
| Carrier Free | IC6655N-100UG | |||
| Carrier Free | IC6655U-100UG | |||
| Carrier Free | IC6655T-100UG | |||
| Carrier Free | IC6655G-100UG | |||
| Carrier Free | IC6655R-100UG | |||
| Carrier Free | MAB6655-SP | |||
![Western Blot: PTEN Antibody (JE51-96) [NBP3-32873] - Western blot analysis of PTEN on different lysates with Rabbit anti-PTEN antibody (NBP3-32873) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: MDA-MB-468 cell lysate (negative) Lane 4: A431 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C6 cell lysate Lane 7: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 47 kDa Observed band size: 55 kDa Exposure time: 2 minutes 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32873) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32873_rabbit-pten-mab-je51-96-288202412461629.jpg "Western Blot: PTEN Antibody (JE51-96) [NBP3-32873] - Western blot analysis of PTEN on different lysates with Rabbit anti-PTEN antibody (NBP3-32873) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: MDA-MB-468 cell lysate (negative) Lane 4: A431 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C6 cell lysate Lane 7: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 47 kDa Observed band size: 55 kDa Exposure time: 2 minutes 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32873) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PTEN Antibody (JE51-96) [NBP3-32873] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-PTEN antibody (NBP3-32873) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32873) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32873_rabbit-pten-mab-je51-96-29820241492644.jpg)
![Flow Cytometry: PTEN Antibody (JE51-96) [NBP3-32873] - Flow cytometric analysis of A431 cells labeling PTEN. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32873, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32873_rabbit-pten-mab-je51-96-29202416372137.jpg)
![Western Blot: PTEN Antibody (RM265) [NBP2-77408] - Western Blot of HeLa and 293 cells lysates using NBP2-77408.](http://images.novusbio.com/fullsize/PTEN-Antibody-RM265-Western-Blot-NBP2-77408-img0002.jpg "Western Blot: PTEN Antibody (RM265) [NBP2-77408] - Western Blot of HeLa and 293 cells lysates using NBP2-77408.")
![Immunohistochemistry-Paraffin: PTEN Antibody (RM265) [NBP2-77408] - Immunohistochemical staining of formalin fixed and paraffin embedded human melanoma sections using NBP2-77408 at a 1:2000 dilution.](http://images.novusbio.com/fullsize/PTEN-Antibody-RM265-Immunohistochemistry-Paraffin-NBP2-77408-img0004.jpg)
![Immunohistochemistry: PTEN Antibody (RM265) [NBP2-77408] - Immunohistochemical staining of formalin fixed and paraffin embedded Human brain tissue sections using NBP2-77408).](http://images.novusbio.com/fullsize/PTEN-Antibody-RM265-Immunohistochemistry-NBP2-77408-img0001.jpg)
![Immunohistochemistry-Paraffin: PTEN Antibody (RM265) [NBP2-77408] - Immunohistochemical staining of formalin fixed and paraffin embedded human prostate cancer sections using NBP2-77408 at a 1:2000 dilution.](http://images.novusbio.com/fullsize/PTEN-Antibody-RM265-Immunohistochemistry-Paraffin-NBP2-77408-img0003.jpg)
![Western Blot: PTENP1 [p Ser29, p Thr32] Antibody [NBP1-77932] - Analysis of Rabbit Anti-Human IL-1 alpha Antibody. Lane 1: Opal Prestained Molecular Weight Ladder. Lane 2: IL-1 alpha 50ng. Lane 3: MEF WC lysate 10ng. Lane 4: MEF LPS stimulated 10ng. Blocking: BlockOut Buffer. Primary Antibody: Anti-IL-1a. Secondary Antibody: Goat anti-Rabbit HRP.](http://images.novusbio.com/fullsize/PTENP1-[p-Ser29--p-Thr32]-Antibody-Western-Blot-NBP1-77932-img0001.jpg "Western Blot: PTENP1 [p Ser29, p Thr32] Antibody [NBP1-77932] - Analysis of Rabbit Anti-Human IL-1 alpha Antibody. Lane 1: Opal Prestained Molecular Weight Ladder. Lane 2: IL-1 alpha 50ng. Lane 3: MEF WC lysate 10ng. Lane 4: MEF LPS stimulated 10ng. Blocking: BlockOut Buffer. Primary Antibody: Anti-IL-1a. Secondary Antibody: Goat anti-Rabbit HRP.")
![Immunocytochemistry/ Immunofluorescence: PTEN [p Ser380] Antibody (JJ08-81) [NBP3-32791] - ICC staining of PTEN in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (NBP3-32791, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32791_rabbit-pten-p-ser380-mab-jj08-81-2920241192374.jpg "Immunocytochemistry/ Immunofluorescence: PTEN [p Ser380] Antibody (JJ08-81) [NBP3-32791] - ICC staining of PTEN in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (NBP3-32791, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).")
![Western Blot: PTEN [p Ser380] Antibody (2E8X8) [NBP3-16911] - Western blot analysis of extracts of 293T cells, using PTEN Rabbit mAb (NBP3-16911) at 1:1000 dilution.293T cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/PTEN-p-Ser380-Antibody-2E8X8-Western-Blot-NBP3-16911-img0001.jpg "Western Blot: PTEN [p Ser380] Antibody (2E8X8) [NBP3-16911] - Western blot analysis of extracts of 293T cells, using PTEN Rabbit mAb (NBP3-16911) at 1:1000 dilution.293T cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3min.")
![ELISA: Human PTEN - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39323] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39323_human-pten-ready-to-use-elisa-kit-colorimetric-2110202415292786.png "ELISA: Human PTEN - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39323] - Standard Curve Reference")
![ELISA: Human PTEN ELISA Kit (Colorimetric) [NBP3-39322] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39322_human-pten-elisa-kit-colorimetric-2110202415302916.png "ELISA: Human PTEN ELISA Kit (Colorimetric) [NBP3-39322] - Standard Curve Reference")