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![Western Blot: Patched 1 Overexpression Lysate (Adult Normal) [NBL1-14924] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Patched 1.](http://images.novusbio.com/fullsize/Patched-1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14924-img0002.jpg "Western Blot: Patched 1 Overexpression Lysate (Adult Normal) [NBL1-14924] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Patched 1.")
![Western Blot: Patched 1/PTCH Antibody (PSH03-53) [NBP3-32718] - Western blot analysis of Patched 1/PTCH on different lysates with Rabbit anti-Patched 1/PTCH antibody (NBP3-32718) at 1/10,000 dilution. Lane 1: 293T-NT cell lysate Lane 2: 293T-OE-PTCH1 cell lysate Lysates/proteins at 1 ug/Lane. Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32718) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32718_rabbit-patched-1-ptch-mab-psh03-53-278202415295590.jpg "Western Blot: Patched 1/PTCH Antibody (PSH03-53) [NBP3-32718] - Western blot analysis of Patched 1/PTCH on different lysates with Rabbit anti-Patched 1/PTCH antibody (NBP3-32718) at 1/10,000 dilution. Lane 1: 293T-NT cell lysate Lane 2: 293T-OE-PTCH1 cell lysate Lysates/proteins at 1 ug/Lane. Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32718) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")