| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26153-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26152-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21558-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26413-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21559-50ul | |||
![Immunoprecipitation: MEK1 Antibody (9B6) [NBP3-26153] - Immunoprecipitating MEK1 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26153 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26153 (2ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (10ug).](http://images.novusbio.com/fullsize/nbp3-26153_rabbit-mek1-mab-9b6-2622024132378.jpg "Immunoprecipitation: MEK1 Antibody (9B6) [NBP3-26153] - Immunoprecipitating MEK1 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26153 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26153 (2ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (10ug).")
![Immunohistochemistry: MEK1 Antibody (9B6) [NBP3-26153] - Image of MEK1 Antibody (9B6) diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26153_rabbit-mek1-mab-9b6-2722024857336.jpg)
![Western Blot: MEK1 Antibody (9B6) [NBP3-26153] - Positive Western Blot detected in: Hela whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, 293T whole cell lysate, A549 whole cell lysate, U251 whole cell lysate, Rat brain tissue. All lanes: MEK1 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 44, 41 kDa Observed band size: 44 kDa](http://images.novusbio.com/fullsize/nbp3-26153_rabbit-mek1-mab-9b6-28220241147210.jpg)
![Immunohistochemistry: MEK1/2 [p Ser221, p Ser217] Antibody - BSA Free [NBP3-20686] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK1/2 (p Ser221, p Ser217).](http://images.novusbio.com/fullsize/nbp3-20686_rabbit-mek1-2-p-ser217-p-ser221-pab-2711202419553094.jpg "Immunohistochemistry: MEK1/2 [p Ser221, p Ser217] Antibody - BSA Free [NBP3-20686] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK1/2 (p Ser221, p Ser217).")
![Western Blot: MEK1/2 [p Ser221, p Ser217] Antibody - BSA Free [NBP3-20686] - Western blot analysis of lysed extracts from HeLa cell untreated or treated with UV MEK1/2 (p Ser221, p Ser217)](http://images.novusbio.com/fullsize/nbp3-20686_rabbit-mek1-2-p-ser217-p-ser221-pab-271120242074623.jpg)
![Immunoprecipitation: MEK1 Antibody (3F10) [NBP3-26152] - Immunoprecipitating MEK1 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26152 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26152 (2ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (10ug).](http://images.novusbio.com/fullsize/nbp3-26152_rabbit-mek1-mab-3f10-2622024132373.jpg "Immunoprecipitation: MEK1 Antibody (3F10) [NBP3-26152] - Immunoprecipitating MEK1 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26152 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26152 (2ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (10ug).")
![Immunohistochemistry: MEK1 Antibody (3F10) [NBP3-26152] - Image of MEK1 Antibody (3F10) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26152_rabbit-mek1-mab-3f10-27220248573314.jpg)
![Western Blot: MEK1 Antibody (3F10) [NBP3-26152] - Positive Western Blot detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, U87 whole cell lysate. All lanes: MEK1 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 44, 41 kDa Observed band size: 44 kDa](http://images.novusbio.com/fullsize/nbp3-26152_rabbit-mek1-mab-3f10-2822024114090.jpg)
![Western Blot: MEK1 [p Ser 298] Antibody (SR1774) [NBP3-21558] - Western blot analysis using NBP3-21558 in (1) HeLa cell treated with LP lysate; (2) HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-21558_rabbit-mek1-p-ser-298-mab-sr1774-57202317161879.jpg "Western Blot: MEK1 [p Ser 298] Antibody (SR1774) [NBP3-21558] - Western blot analysis using NBP3-21558 in (1) HeLa cell treated with LP lysate; (2) HeLa cell lysate.")
![Western Blot: MEK1 [p Ser218, p Ser222] Antibody [NB500-139] - Western Blot of NIH 3T3 cell lysates showing specific immunolabeling of the ~45kDa MEK 1/2 protein phosphorylated at Ser218 and Ser222. The cells were either serum starved (Control) or incubated in the presence of serum (Serum). Immunolabeling of an additional band at ~95kDa was also observed.](http://images.novusbio.com/fullsize/MEK1-[p-Ser218-p-Ser222]-Antibody-Western-Blot-NB500-139-img0001.jpg "Western Blot: MEK1 [p Ser218, p Ser222] Antibody [NB500-139] - Western Blot of NIH 3T3 cell lysates showing specific immunolabeling of the ~45kDa MEK 1/2 protein phosphorylated at Ser218 and Ser222. The cells were either serum starved (Control) or incubated in the presence of serum (Serum). Immunolabeling of an additional band at ~95kDa was also observed.")
![Immunoprecipitation: MEK1 Antibody [NBP3-14688] - : Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from HeLa cells preparedusing NETN lysis buffer. Antibodies: Rabbit anti-MEK1recombinant monoclonal antibody used for IP at 20 ul/mg lysate. MEK1 was alsoimmunoprecipitated by rabbit anti-MEK1 recombinantmonoclonal antibody. For blottingimmunoprecipitated MEK1, was used at 1:1000.Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/MEK1-Antibody-Immunoprecipitation-NBP3-14688-img0001.jpg "Immunoprecipitation: MEK1 Antibody [NBP3-14688] - : Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from HeLa cells preparedusing NETN lysis buffer. Antibodies: Rabbit anti-MEK1recombinant monoclonal antibody used for IP at 20 ul/mg lysate. MEK1 was alsoimmunoprecipitated by rabbit anti-MEK1 recombinantmonoclonal antibody. For blottingimmunoprecipitated MEK1, was used at 1:1000.Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: MEK1/2 Antibody [NBP3-05651] - Analysis of extracts of various cell lines, using MAP2K1/MAP2K2 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/MEK1-2-Antibody-Western-Blot-NBP3-05651-img0002.jpg "Western Blot: MEK1/2 Antibody [NBP3-05651] - Analysis of extracts of various cell lines, using MAP2K1/MAP2K2 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 60s.")
![Western Blot: MEK1/2 Antibody [NBP3-05651] - Analysis of extracts of PC-12 cells, using MEK1/2 antibody (NBP3-05651) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/MEK1-2-Antibody-Western-Blot-NBP3-05651-img0001.jpg)
![SDS-Page: Recombinant Human MEK1 Protein [H00005604-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00005604-Q01-1.jpg "SDS-Page: Recombinant Human MEK1 Protein [H00005604-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: MEK1 Overexpression Lysate (Adult Normal) [NBL1-12845] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MEK1.](http://images.novusbio.com/fullsize/MEK1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12845-img0002.jpg "Western Blot: MEK1 Overexpression Lysate (Adult Normal) [NBL1-12845] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MEK1.")
![Western Blot: MEK1 [p Thr292] Antibody (4E7) [NBP3-26413] - Positive Western Blot detected in Hela whole cell lysate. All lanes: MEK1 [p Thr292] Antibody at 1.645ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 45 KDa Observed band size: 45 KDa](http://images.novusbio.com/fullsize/nbp3-26413_rabbit-mek1-p-thr-292-mab-4e7-282202411442628.jpg "Western Blot: MEK1 [p Thr292] Antibody (4E7) [NBP3-26413] - Positive Western Blot detected in Hela whole cell lysate. All lanes: MEK1 [p Thr292] Antibody at 1.645ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 45 KDa Observed band size: 45 KDa")
![Western Blot: MEK1 [p Thr 292] Antibody (SR1773) [NBP3-21559] - Western blot analysis using NBP3-21559 in (1) HeLa cell lysate; (2) HeLa cell treated with Nocodazole.](http://images.novusbio.com/fullsize/nbp3-21559_rabbit-mek1-p-thr-292-mab-sr1773-57202317215122.jpg "Western Blot: MEK1 [p Thr 292] Antibody (SR1773) [NBP3-21559] - Western blot analysis using NBP3-21559 in (1) HeLa cell lysate; (2) HeLa cell treated with Nocodazole.")